公开(公告)号：US20150079652A1

公开(公告)日：2015-03-19

申请号：US14486377

申请日：2014-09-15

Applicant: SHELL OIL COMPANY

Inventor： Annette Victoria Angeles ALCASABAS ,  Nadim ASHRAF ,  George Robert LEE ,  David Alexander PARKER

IPC: C12P7/06 ,  C12N1/16

CPC classification number: C12P7/06 ,  C12N1/16 ,  C12N1/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/93 ,  C12P7/10 ,  C12Y101/01001 ,  C12Y102/0101 ,  C12Y602/01001 ,  Y02E50/16 ,  Y02E50/17

Abstract: Sacchromyces cerevisiae strains modified to express an adhE (alcohol/aldehyde dehydrogenase) enzyme having the amino acid sequence SEQ ID NO:1, or a functional variant thereof capable of catalysing the conversion of acetyl CoA to ethanol, and/or to overexpress an ACS2 (acetyl-CoA synthetase) enzyme having the amino acid sequence SEQ ID NO:2, or a functional variant thereof capable of catalysing the conversion of acetate to acetyl CoA. Such cells have an improved ability to grow on or in acetate-containing media compared to cells not according to the invention.

Abstract translation: 酿酒酵母菌株被修饰以表达具有氨基酸序列SEQ ID NO：1的adhE（醇/醛脱氢酶）或其能够催化乙酰CoA转化为乙醇的功能变体，和/或过表达ACS2（ 具有氨基酸序列SEQ ID NO：2的乙酰辅酶A合成酶）或其能够催化乙酸转化为乙酰辅酶A的功能变体。 与不是本发明的细胞相比，这样的细胞具有改善的在含乙酸酯培养基上或含乙酸酯培养基中生长的能力。

公开(公告)号：US20140193873A1

公开(公告)日：2014-07-10

申请号：US14208857

申请日：2014-03-13

Applicant: SHELL OIL COMPANY

Inventor： Thomas Paul HOWARD ,  Sabine MIDDELHAUFE ,  Dagmara KOLAK ,  Stephen J. AVES ,  John LOVE ,  David PARKER ,  George Robert LEE

IPC: C12P5/02

CPC classification number: C12P5/02 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12P5/026 ,  C12P7/24 ,  C12Y102/0105 ,  C12Y203/01 ,  C12Y301/02014 ,  C12Y401/99005 ,  C12Y602/01019

Abstract: A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-ACP, or a fatty acyl-CoA or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme.

Abstract translation: 一种制备烃的方法，包括使脂肪酸底物与至少一种脂肪酸还原酶和至少一种脂肪醛合成酶和至少一种脂肪酰基转移酶接触，其中所述脂肪酸底物是脂肪酸，脂肪酰基-ACP， 或脂肪酰基辅酶A或它们的混合物，得到脂肪醛; 并使脂肪醛与至少一种醛脱羰酶接触。

公开(公告)号：US20140059923A1

公开(公告)日：2014-03-06

申请号：US14018766

申请日：2013-09-05

Applicant: SHELL OIL COMPANY

Inventor： Aldo CAIAZZO ,  Lionel CLARKE ,  Tor Kit GOH ,  George Robert LEE ,  David Alexander PARKER ,  Richard John PRICE

IPC: C10L1/185

CPC classification number: C10L1/1852 ,  C10L1/02 ,  C10L1/026 ,  C10L10/12 ,  C10L2200/04 ,  C10L2200/0446 ,  C10L2200/0476 ,  Y02E50/13

Abstract: A fuel composition comprising: a diesel base fuel; from 1 to 10% v/v of a fatty acid alkyl ester; and more than 10% v/v of an ether component, the ether component comprising one or more ether compounds having in the range of from 8 to 12 carbon atoms and selected from compounds of formula I R1—O—R2  (I) wherein R1 and R2 are independently primary or secondary alkyl.

Abstract translation: 一种燃料组合物，包括：柴油基础燃料; 1至10％v / v的脂肪酸烷基酯; 和超过10％v / v的醚组分，所述醚组分包含一种或多种在8至12个碳原子范围内的醚化合物，并且选自式I的化合物R 1 -O-R 2（I）其中R 1 且R2独立地为伯或仲烷基。

公开(公告)号：US20140024090A1

公开(公告)日：2014-01-23

申请号：US13715502

申请日：2012-12-14

Applicant: SHELL OIL COMPANY

Inventor： Steven Daniel DOIG ,  Steven Richard BROWN ,  George Robert LEE ,  David Alexander PARKER

IPC: C12P7/00 ,  C12M1/00 ,  C12P5/00

CPC classification number: C12P7/00 ,  B01F3/04531 ,  B01F5/10 ,  B01F5/106 ,  B01F2003/04673 ,  C10G3/50 ,  C12M21/00 ,  C12M21/12 ,  C12M29/18 ,  C12M29/26 ,  C12P5/00 ,  Y02E50/13 ,  Y02P30/20 ,  Y10T29/49826

Abstract: Method for converting a carbon source such as a plant-derived material into a hydrocarbon or hydrocarbon precursor, via a microorganism-catalysed aerobic reaction. The microorganism is cultivated in the presence of the carbon source and of oxygen, in a reaction vessel having a capacity of about 2000 litres or greater which comprises an aeration system for supplying oxygen to the microorganism. Also provided is an apparatus for use in the method, comprising a reaction vessel of capacity 2000 litres or greater, and an aeration system.

Abstract translation: 通过微生物催化的需氧反应将诸如植物衍生物质的碳源转化成烃或烃前体的方法。 在碳源和氧的存在下，在具有约2000升或更高的容量的反应容器中培养微生物，其包含用于向微生物供氧的曝气系统。 还提供了一种用于该方法的装置，包括容积为2000升或更大的反应容器和曝气系统。

公开(公告)号：US20130224818A1

公开(公告)日：2013-08-29

申请号：US13774647

申请日：2013-02-22

Applicant: Shell Oil Company

Inventor： Thomas Paul HOWARD ,  Sabine MIDDELHAUFE ,  Dagmara KOLAK ,  Stephen J. AVES ,  John LOVE ,  David PARKER ,  George Robert LEE

IPC: C12P5/02

CPC classification number: C12P5/02 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12P5/026 ,  C12P7/24 ,  C12Y102/0105 ,  C12Y203/01 ,  C12Y301/02014 ,  C12Y401/99005 ,  C12Y602/01019

Abstract: A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-ACP, or a fatty acyl-CoA or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme.

Abstract translation: 一种制备烃的方法，包括使脂肪酸底物与至少一种脂肪酸还原酶和至少一种脂肪醛合成酶和至少一种脂肪酰基转移酶接触，其中所述脂肪酸底物是脂肪酸，脂肪酰基-ACP， 或脂肪酰基辅酶A或它们的混合物，得到脂肪醛; 并使脂肪醛与至少一种醛脱羰酶接触。