公开(公告)号：US20150079652A1

公开(公告)日：2015-03-19

申请号：US14486377

申请日：2014-09-15

Applicant: SHELL OIL COMPANY

Inventor： Annette Victoria Angeles ALCASABAS ,  Nadim ASHRAF ,  George Robert LEE ,  David Alexander PARKER

IPC: C12P7/06 ,  C12N1/16

CPC classification number: C12P7/06 ,  C12N1/16 ,  C12N1/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/93 ,  C12P7/10 ,  C12Y101/01001 ,  C12Y102/0101 ,  C12Y602/01001 ,  Y02E50/16 ,  Y02E50/17

Abstract: Sacchromyces cerevisiae strains modified to express an adhE (alcohol/aldehyde dehydrogenase) enzyme having the amino acid sequence SEQ ID NO:1, or a functional variant thereof capable of catalysing the conversion of acetyl CoA to ethanol, and/or to overexpress an ACS2 (acetyl-CoA synthetase) enzyme having the amino acid sequence SEQ ID NO:2, or a functional variant thereof capable of catalysing the conversion of acetate to acetyl CoA. Such cells have an improved ability to grow on or in acetate-containing media compared to cells not according to the invention.

Abstract translation: 酿酒酵母菌株被修饰以表达具有氨基酸序列SEQ ID NO：1的adhE（醇/醛脱氢酶）或其能够催化乙酰CoA转化为乙醇的功能变体，和/或过表达ACS2（ 具有氨基酸序列SEQ ID NO：2的乙酰辅酶A合成酶）或其能够催化乙酸转化为乙酰辅酶A的功能变体。 与不是本发明的细胞相比，这样的细胞具有改善的在含乙酸酯培养基上或含乙酸酯培养基中生长的能力。