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1.发明申请公开(公告)号：US20170220000A1
公开(公告)日：2017-08-03
申请号：US15500880
申请日：2015-07-31
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Alon Greenbaum , Yibo Zhang , Alborz Feizi , Wei Luo
CPC classification number: G03H1/0005 , G01N15/1429 , G01N15/1434 , G01N15/1475 , G01N2015/1006 , G01N2015/144 , G01N2015/1445 , G01N2015/1454 , G03H1/0443 , G03H1/0866 , G03H1/0891 , G03H2001/005 , G03H2001/0447 , G03H2001/0454 , G03H2001/046 , G03H2001/0816 , G03H2001/0883 , G03H2001/2655 , G03H2210/42 , G03H2226/02 , G03H2227/03 , H04N5/2256 , H04N5/23238
Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.
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公开(公告)号：US20170160197A1
公开(公告)日：2017-06-08
申请号：US15368420
申请日：2016-12-02
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Qingshan Wei , Wei Luo
CPC classification number: G06K9/00134 , G01N21/6456 , G01N21/6458 , G06K9/0014 , G06T7/60 , G06T2207/10024 , G06T2207/10064 , G06T2207/30072
Abstract: A device and method for imaging fluorescently labeled molecules (e.g., nucleic acids) includes securing a modular attachment device to the mobile phone with a sample containing stretched, fluorescently labeled nucleic acid molecules and illuminating the sample with excitation light to cause the fluorescently labeled nucleic acid molecules to emit fluorescent light. Images of the nucleic acids are captured using a camera of the mobile phone. The images from the mobile phone are transferred to a remote computer for image processing and analysis. The images are processed by the remote computer to generate analysis data of sample, wherein the analysis data includes the length of nucleic acid molecules contained in the sample or the length of molecular sub-region(s). The mobile phone or another computing device receives from the remote computer the analysis data and displays at least some of the analysis data thereon.
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3.发明授权公开(公告)号：US11422503B2
公开(公告)日：2022-08-23
申请号：US16952492
申请日：2020-11-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Alon Grinbaum , Yibo Zhang , Alborz Feizi , Wei Luo
Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.
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Patent Agency Ranking
公开(公告)号：US10248838B2
公开(公告)日：2019-04-02
申请号：US15368420
申请日：2016-12-02
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Qingshan Wei , Wei Luo
Abstract: A device and method for imaging fluorescently labeled molecules (e.g., nucleic acids) includes securing a modular attachment device to the mobile phone with a sample containing stretched, fluorescently labeled nucleic acid molecules and illuminating the sample with excitation light to cause the fluorescently labeled nucleic acid molecules to emit fluorescent light. Images of the nucleic acids are captured using a camera of the mobile phone. The images from the mobile phone are transferred to a remote computer for image processing and analysis. The images are processed by the remote computer to generate analysis data of sample, wherein the analysis data includes the length of nucleic acid molecules contained in the sample or the length of molecular sub-region(s). The mobile phone or another computing device receives from the remote computer the analysis data and displays at least some of the analysis data thereon.
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公开(公告)号：US10795315B2
公开(公告)日：2020-10-06
申请号：US16300546
申请日：2017-05-10
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Yichen Wu , Yibo Zhang , Wei Luo
IPC: G06K9/76 , G03H1/04 , H04N9/04 , G03H1/00 , G03H1/22 , G06T3/40 , H04N5/232 , H04N9/68 , H04N9/73
Abstract: A method of generating a color image of a sample includes obtaining a plurality of low resolution holographic images of the sample using a color image sensor, the sample illuminated simultaneously by light from three or more distinct colors, wherein the illuminated sample casts sample holograms on the image sensor and wherein the plurality of low resolution holographic images are obtained by relative x, y, and z directional shifts between sample holograms and the image sensor. Pixel super-resolved holograms of the sample are generated at each of the three or more distinct colors. De-multiplexed holograms are generated from the pixel super-resolved holograms. Phase information is retrieved from the de-multiplexed holograms using a phase retrieval algorithm to obtain complex holograms. The complex hologram for the three or more distinct colors is digitally combined and back-propagated to a sample plane to generate the color image.
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6.发明申请公开(公告)号：US20210181673A1
公开(公告)日：2021-06-17
申请号：US16952492
申请日：2020-11-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Alon Grinbaum , Yibo Zhang , Alborz Feizi , Wei Luo
Abstract: A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.
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公开(公告)号：US20190286053A1
公开(公告)日：2019-09-19
申请号：US16300546
申请日：2017-05-10
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Yichen Wu , Yibo Zhang , Wei Luo
Abstract: A method of generating a color image of a sample includes obtaining a plurality of low resolution holographic images of the sample using a color image sensor, the sample illuminated simultaneously by light from three or more distinct colors, wherein the illuminated sample casts sample holograms on the image sensor and wherein the plurality of low resolution holographic images are obtained by relative x, y, and z directional shifts between sample holograms and the image sensor. Pixel super-resolved holograms of the sample are generated at each of the three or more distinct colors. De-multiplexed holograms are generated from the pixel super-resolved holograms. Phase information is retrieved from the de-multiplexed holograms using a phase retrieval algorithm to obtain complex holograms. The complex hologram for the three or more distinct colors is digitally combined and back-propagated to a sample plane to generate the color image.
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公开(公告)号：US20170168285A1
公开(公告)日：2017-06-15
申请号：US15379143
申请日：2016-12-14
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Aydogan Ozcan , Wei Luo
CPC classification number: G02B21/367 , G02B21/0008 , G02B21/0056 , G02B21/365 , G03H1/0443 , G03H1/0866 , G03H2001/0447 , G03H2001/046 , G03H2222/34 , H04N5/2256
Abstract: A method for obtaining a high resolution image of objects contained within a sample is disclosed that combines pixel super-resolution and phase retrieval techniques into a unified algorithmic framework that enables new holographic image reconstruction methods with significantly improved data efficiency, i.e., using much less number of raw measurements to obtain high-resolution and wide-field reconstructions of the sample. Using the unified algorithmic framework, twin image noise and spatial aliasing signals, along with other digital holographic artifacts, can be interpreted as noise terms modulated by digital phasors, which are all analytical functions of the imaging parameters including e.g., the lateral displacement between the hologram and the sensor array planes (x, y shifts), sample-to-image sensor distance (z), illumination wavelength (λ), and the angle of incidence (θ,φ).
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