公开(公告)号：US20190170649A1

公开(公告)日：2019-06-06

申请号：US16210311

申请日：2018-12-05

Applicant: THE UNIVERSITY OF TOKYO

Inventor： Yuichi WAKAMOTO ,  Koseki KOBAYASHI

IPC: G01N21/65 ,  G01J3/44 ,  G01L1/24 ,  C12Q1/6816

CPC classification number: G01N21/65 ,  C12Q1/6816 ,  G01J3/44 ,  G01L1/242 ,  G01N2021/0143 ,  G01N2201/067

Abstract: An objective cell is irradiated with laser beam of a predetermined wavelength. Only Stokes light is selected out of detected light including reflected light and scattered light of the laser beam, and a Raman scattering spectrum is obtained by dispersion of the selected Stokes light. A transcriptome of the objective cells is estimated, based on the Raman scattering spectrum. It is preferable to estimate the transcriptome of the objective cells, based on N-dimensional Raman data obtained by dimensional reduction of the Raman scattering spectrum. This configuration only needs to irradiate the objective cell with the laser beam and does not require to destroy the objective cell. As a result, this enables the transcriptome of the cell to be estimated in a short time period without destroying the cell.