公开(公告)号：US20250164470A1

公开(公告)日：2025-05-22

申请号：US19041116

申请日：2025-01-30

Applicant: TOPPAN HOLDINGS INC.

Inventor： Yuji KUBO ,  Yoichi MAKINO

IPC: G01N33/53 ,  C12Q1/6804

Abstract: A method of evaluating whether a protein of interest binds to a target protein includes bringing a protein of interest, a target protein, a first specific binding substance which is for the protein of interest and is labeled with a first single-stranded nucleic acid fragment, and a second specific binding substance which is for the target protein and is labeled with a second single-stranded nucleic acid fragment, into contact with each other in a container, and forming as a result a complex including the protein of interest, target protein, and first and second specific binding substances when the protein of interest binds to the target protein, with a double-stranded nucleic acid being formed by hybridization of the first fragment with the second fragment; and detecting formation of the double-stranded nucleic acid. Detection of formation of the double-stranded nucleic acid indicates binding of the protein of interest to the target protein.

公开(公告)号：US20250123207A1

公开(公告)日：2025-04-17

申请号：US19002825

申请日：2024-12-27

Applicant: TOPPAN HOLDINGS INC.

Inventor： Yuta SUZUKI ,  Yoichi MAKINO

IPC: G01N21/64

Abstract: A detection device includes a temperature adjustment unit that adjusts a temperature of a fluidic device, an imaging unit that irradiates the fluidic device with excitation light and captures an image of fluorescence emitted from the fluidic device, and a conveying unit that conveys the fluidic device to the temperature adjustment unit and the imaging unit. The fluidic device houses a mixed aqueous solution of a liquid sample that contains a target substance and a detection reagent that reacts with the target substance to generate a product that emits the fluorescence, and the temperature adjustment unit includes a heating unit that heats the fluidic device and a cooling unit that cools the fluidic device heated.

公开(公告)号：US20240280592A1

公开(公告)日：2024-08-22

申请号：US18651906

申请日：2024-05-01

Applicant: TOPPAN Holdings Inc. ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY

Inventor： Yoichi MAKINO ,  Takumi HIRASE ,  Kohei YUYAMA

IPC: G01N33/68 ,  G01N33/543

CPC classification number: G01N33/6896 ,  G01N33/54326 ,  G01N2333/28 ,  G01N2333/4709 ,  G01N2458/10

Abstract: There are provided a method for detecting amyloid-β bound to a brain-neuronal-cell-derived exosome in blood, the method including: a step of binding amyloid-β bound to the brain-neuronal-cell-derived exosome in blood to an amyloid-β-specific binding substance that specifically binds to amyloid-β, labeled with a nucleic acid fragment; and a step of detecting the amyloid-β-specific binding substance, and a method for evaluating an accumulation level of amyloid-β in a brain, the method including: a step of detecting amyloid-β bound to the brain-neuronal-cell-derived exosome in blood; and a step of evaluating an accumulation level of amyloid-β in a brain based on the detected amount of the amyloid-β.

公开(公告)号：US20240279717A1

公开(公告)日：2024-08-22

申请号：US18652258

申请日：2024-05-01

Applicant: TOPPAN HOLDINGS INC.

Inventor： Yuji KUBO ,  Yoichi MAKINO

IPC: C12Q1/6818 ,  G01N33/577 ,  G01N33/68

CPC classification number: C12Q1/6818 ,  G01N33/577 ,  G01N33/6854

Abstract: A method of detecting a target substance in a sample includes introducing into wells the sample, a first specific binding substance which is for the target substance and labeled with a first single-stranded nucleic acid fragment, and a second specific binding substance which is for the target substance and labeled with a second single-stranded nucleic acid fragment, forming as a result a complex containing the target substance, the first specific binding substance, and the second specific binding substance if the target substance is present in the sample, and forming a double-stranded nucleic acid by hybridization of at least part of the first single-stranded nucleic acid fragment with at least part of the second single-stranded nucleic acid fragment; and detecting formation of the double-stranded nucleic acid, wherein detection of formation of the double-stranded nucleic acid indicates presence of the target substance.

公开(公告)号：US20250115949A1

公开(公告)日：2025-04-10

申请号：US18986953

申请日：2024-12-19

Applicant: TOPPAN HOLDINGS INC.

Inventor： Masayuki OGINO ,  Misaki NAGAHARA ,  Yoichi MAKINO ,  Yosuke HORIUCHI ,  Yuta SUZUKI

IPC: C12Q1/6818 ,  G01N21/64

Abstract: A single-stranded oligonucleotide labeled with a fluorescent substance and a quenching substance. In the single-stranded oligonucleotide, a hairpin structure is formed by self-hybridization on the 5′ side, and when a single-stranded oligonucleotide having a complementary base sequence on the 3′ side hybridizes, a triplex structure is formed on the 5′ end portion, and cleaved by a flap endonuclease that recognizes the triplex structure, whereby the fluorescent substance is released from the quenching substance, the fluorescent substance emitting fluorescence when irradiated with excitation light, and the single-stranded nucleotide is a single-stranded oligonucleotide including a non-complementary base pair within the hairpin structure, a single-stranded oligonucleotide in which a nucleotide residue labeled with the fluorescent substance and a nucleotide residue labeled with the quenching substance are separated from each other by 4 or more residues, or a single-stranded oligonucleotide in which the fluorescent substance is bound to a portion other than the base.

公开(公告)号：US20250084460A1

公开(公告)日：2025-03-13

申请号：US18960717

申请日：2024-11-26

Applicant: TOPPAN HOLDINGS INC.

Inventor： Masayuki OGINO ,  Yoichi MAKINO

IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6837 ,  C12Q1/6876

Abstract: A method of detecting a nucleic acid including mixing a fluid including a target nucleic acid with a detection reagent including a first enzyme for cleaving a first nucleic acid having a first flap and a second enzyme for cleaving a second nucleic acid such that the target nucleic acid, the first nucleic acid and the second nucleic acid form a complex as a first invasive structure, conducting a first reaction which causes the first enzyme to cleave the first flap of the first invasive structure and produces a third nucleic acid that forms a complex, as a second invasive structure, with a fourth nucleic acid having a second flap, and conducting a second reaction which causes the second enzyme to cleave the second flap of the second invasive structure and produces a cleaved product.