公开(公告)号：US20250164470A1

公开(公告)日：2025-05-22

申请号：US19041116

申请日：2025-01-30

Applicant: TOPPAN HOLDINGS INC.

Inventor： Yuji KUBO ,  Yoichi MAKINO

IPC: G01N33/53 ,  C12Q1/6804

Abstract: A method of evaluating whether a protein of interest binds to a target protein includes bringing a protein of interest, a target protein, a first specific binding substance which is for the protein of interest and is labeled with a first single-stranded nucleic acid fragment, and a second specific binding substance which is for the target protein and is labeled with a second single-stranded nucleic acid fragment, into contact with each other in a container, and forming as a result a complex including the protein of interest, target protein, and first and second specific binding substances when the protein of interest binds to the target protein, with a double-stranded nucleic acid being formed by hybridization of the first fragment with the second fragment; and detecting formation of the double-stranded nucleic acid. Detection of formation of the double-stranded nucleic acid indicates binding of the protein of interest to the target protein.

公开(公告)号：US20240279717A1

公开(公告)日：2024-08-22

申请号：US18652258

申请日：2024-05-01

Applicant: TOPPAN HOLDINGS INC.

Inventor： Yuji KUBO ,  Yoichi MAKINO

IPC: C12Q1/6818 ,  G01N33/577 ,  G01N33/68

CPC classification number: C12Q1/6818 ,  G01N33/577 ,  G01N33/6854

Abstract: A method of detecting a target substance in a sample includes introducing into wells the sample, a first specific binding substance which is for the target substance and labeled with a first single-stranded nucleic acid fragment, and a second specific binding substance which is for the target substance and labeled with a second single-stranded nucleic acid fragment, forming as a result a complex containing the target substance, the first specific binding substance, and the second specific binding substance if the target substance is present in the sample, and forming a double-stranded nucleic acid by hybridization of at least part of the first single-stranded nucleic acid fragment with at least part of the second single-stranded nucleic acid fragment; and detecting formation of the double-stranded nucleic acid, wherein detection of formation of the double-stranded nucleic acid indicates presence of the target substance.