公开(公告)号：US20220002645A1

公开(公告)日：2022-01-06

申请号：US17481354

申请日：2021-09-22

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Tomoko KUNITOMI ,  Yoichi MAKINO

IPC: C12M1/34 ,  C12M1/00 ,  G01N21/64 ,  B01L3/00 ,  C12Q1/6825

Abstract: A method for filling an analytical device includes providing a plate having a detection space or spaces and ports communicating with the space(s), fitting, into one of the ports, an adapter having a reagent fixed on an inner wall of the adapter such that a first end of the adapter is fitted into the port, and delivering a sample solution into the detection space(s) through the adapter such that the sample solution and reagent fill the detection space(s). The plate includes a first substrate, a second substrate, and a side wall such that the plate has the detection space(s) between the substrates and that the first substrate has the ports, the first and/or second substrates is formed of transparent material such that the detection space is observable from outside the plate, and the ports include a first port for delivering liquid-containing substance, and a second port for discharging the substance.

公开(公告)号：US20190060897A1

公开(公告)日：2019-02-28

申请号：US16173102

申请日：2018-10-29

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi MAKINO ,  Akihiro HOSHINO ,  Tomoko KUNITOMI ,  Keisuke GOTO ,  Yosuke HORIUCHI

IPC: B01L3/00 ,  G01N21/64

Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.

公开(公告)号：US20220290222A1

公开(公告)日：2022-09-15

申请号：US17751031

申请日：2022-05-23

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Yoichi MAKINO ,  Tomoko KUNITOMI

IPC: C12Q1/6858 ,  B01L3/00 ,  G01N21/64 ,  C12Q1/686

Abstract: A biomolecule analysis method may include preparing a reaction container comprising an array of a plurality of micropores in which a wash buffer is filled or preparing the reaction container and feeding the wash buffer into the reaction container including the array of the plurality of micropores. The method may include feeding a reaction reagent to detect a sample, into the plurality of micropores, the sample including a biomolecule. The method may include feeding a sealing solution to seal the plurality of micropores so that the plurality of micropores become a plurality of independent reaction chambers sealed from each other. The method may include causing an isothermal detection reaction in each of the plurality of the independent reaction containers. The method may include detecting a signal amplified by the isothermal detection reaction.

公开(公告)号：US20200003691A1

公开(公告)日：2020-01-02

申请号：US16570628

申请日：2019-09-13

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Misaki NAGAHARA ,  Yoichi MAKINO ,  Akihiro HOSHINO ,  Takumi HIRASE ,  Tomoko KUNITOMI ,  Keisuke GOTO ,  Yusuke HORIUCHI

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/76 ,  C12Q1/6825

Abstract: A device including a well array including wells in which a target substance is detected based on a biochemical reaction thereof. Each of the wells has an aspect ratio of 1 or more as calculated by Formula (1) such that the biochemical reaction of the target substance is detected, Aspect ratio=Depth of each well/Diameter of largest circle among circles included in a region enclosed by a rim of each well  Formula (1).

公开(公告)号：US20180119078A1

公开(公告)日：2018-05-03

申请号：US15854355

申请日：2017-12-26

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Tomoko KUNITOMI ,  Yoichi MAKINO

IPC: C12M1/34 ,  G01N21/64 ,  C12Q1/6825

CPC classification number: C12M1/34 ,  B01L3/50851 ,  B01L3/563 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/0654 ,  B01L2300/069 ,  B01L2300/0819 ,  C12M1/00 ,  C12Q1/6825 ,  G01N21/64 ,  G01N21/6452

Abstract: An analytical device includes a plate including a first substrate having a front surface and a back surface and a second substrate facing the back surface of the first substrate such that at least one detection space observable from outside is formed between the second substrate and the back surface of the first substrate, and an adapter. The first substrate has a first port having a through hole formed through the front surface and communicated with the detection space for delivering a liquid-containing substance into the detection space, the first substrate has a second port having a through hole formed through the front surface and communicated with the detection space for discharging a liquid-containing substance or a gas from the detection space, and the adapter is attachable to and detachable from the first port and the second port, and the front surface of the first substrate without the adapter is flat.