公开(公告)号：US20200270674A1

公开(公告)日：2020-08-27

申请号：US16872855

申请日：2020-05-12

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Takumi HIRASE ,  Yoichi MAKINO ,  Yosuke HORIUCHI

IPC: C12Q1/6806 ,  C12Q1/686 ,  G01N33/536 ,  B01L3/00

Abstract: A method for detecting a target molecule, including supplying a liquid dispersion of a structural substance including a target molecule into a well array such that the structural substance is introduced into wells of the well array, supplying a sealing solution into the well array such that a layer of the sealing solution is formed on the liquid dispersion in the wells, and that the liquid dispersion is sealed in the wells, extracting the target molecule from the structural substance, and detecting the target molecule.

公开(公告)号：US20240175074A1

公开(公告)日：2024-05-30

申请号：US18402807

申请日：2024-01-03

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Takumi HIRASE ,  Yoichi MAKINO ,  Yosuke HORIUCHI

IPC: C12Q1/6806 ,  B01L3/00 ,  C12Q1/686 ,  G01N33/536

CPC classification number: C12Q1/6806 ,  B01L3/5027 ,  C12Q1/686 ,  G01N33/536 ,  B01L2200/0689 ,  B01L2300/0829

Abstract: A method for detecting a target molecule, including supplying a liquid dispersion of a structural substance including a target molecule into a well array such that the structural substance is introduced into wells of the well array, supplying a sealing solution into the well array such that a layer of the sealing solution is formed on the liquid dispersion in the wells, and that the liquid dispersion is sealed in the wells, extracting the target molecule from the structural substance, and detecting the target molecule.

公开(公告)号：US20150125941A1

公开(公告)日：2015-05-07

申请号：US14589492

申请日：2015-01-05

Applicant: TOPPAN PRINTING CO., LTD. ,  TOKYO INSTITUTE OF TECHNOLOGY

Inventor： Takumi HIRASE ,  Masato Nakayama ,  Hiroshi Handa ,  Satoshi Sakamoto ,  Yasuyuki Naito

IPC: G01N33/543 ,  G01N21/64

CPC classification number: G01N33/54326 ,  G01N21/6428 ,  G01N21/645

Abstract: A detection system of a test substance, includes: a magnetic body that is modified by a first substance capable of being bound to the test substance at a first binding site of the test substance; a marker that is modified by a second substance capable of being bound to the test substance at a second binding site of the test substance, the second coupling site being a different site from the first binding site; a development medium that has a flow passage into which liquid, in which the magnetic body and the marker is dispersed, flows due to a capillary phenomenon, the development medium having a detection position for detecting the marker in a portion of the flow passage, the development medium having light transparency at least in the detection position; and a magnetic force generating portion that is equipped in the development medium.

Abstract translation: 测试物质的检测系统包括：被测物质的第一结合位点被能够与被检物质结合的第一物质改性的磁性体; 能够在被检物质的第二结合位点与被检物质结合的第二物质修饰的标记，第二偶联位点是与第一结合位点不同的位点; 具有流路的开发介质，由于毛细管现象，磁性体和标记物分散在其中的液体流动，显影介质具有用于检测流路的一部分中的标记的检测位置， 至少在检测位置具有透光性的显影介质; 以及设置在显影介质中的磁力产生部。

公开(公告)号：US20200003691A1

公开(公告)日：2020-01-02

申请号：US16570628

申请日：2019-09-13

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Misaki NAGAHARA ,  Yoichi MAKINO ,  Akihiro HOSHINO ,  Takumi HIRASE ,  Tomoko KUNITOMI ,  Keisuke GOTO ,  Yusuke HORIUCHI

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/76 ,  C12Q1/6825

Abstract: A device including a well array including wells in which a target substance is detected based on a biochemical reaction thereof. Each of the wells has an aspect ratio of 1 or more as calculated by Formula (1) such that the biochemical reaction of the target substance is detected, Aspect ratio=Depth of each well/Diameter of largest circle among circles included in a region enclosed by a rim of each well  Formula (1).

公开(公告)号：US20170128904A1

公开(公告)日：2017-05-11

申请号：US15412908

申请日：2017-01-23

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Takumi HIRASE ,  Rii Morimura ,  Masato Nakayama

IPC: B01J13/20 ,  G01N21/64 ,  C09K11/06 ,  G01N33/92 ,  G01N33/58

Abstract: In methods of separating, moving, and detecting a vesicle of the present invention, either a vesicular-shaped lipid membrane structure containing a membrane-fusogenic lipid capable of being fused with a vesicle having a lipid bilayer membrane, or a lipid-membrane-structure-immobilization carrier, in which a lipid membrane structure containing a membrane-fusogenic lipid capable of being fused with a vesicle having a lipid bilayer membrane is immobilized on a carrier, is brought into contact with a sample containing the vesicle such that membrane fusion occurs between the lipid membrane structure and the vesicle.