-
公开(公告)号:US20200003691A1
公开(公告)日:2020-01-02
申请号:US16570628
申请日:2019-09-13
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Misaki NAGAHARA , Yoichi MAKINO , Akihiro HOSHINO , Takumi HIRASE , Tomoko KUNITOMI , Keisuke GOTO , Yusuke HORIUCHI
IPC: G01N21/64 , B01L3/00 , G01N21/76 , C12Q1/6825
Abstract: A device including a well array including wells in which a target substance is detected based on a biochemical reaction thereof. Each of the wells has an aspect ratio of 1 or more as calculated by Formula (1) such that the biochemical reaction of the target substance is detected, Aspect ratio=Depth of each well/Diameter of largest circle among circles included in a region enclosed by a rim of each well Formula (1).
-
公开(公告)号:US20180119078A1
公开(公告)日:2018-05-03
申请号:US15854355
申请日:2017-12-26
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Tomoko KUNITOMI , Yoichi MAKINO
IPC: C12M1/34 , G01N21/64 , C12Q1/6825
CPC classification number: C12M1/34 , B01L3/50851 , B01L3/563 , B01L7/52 , B01L2200/026 , B01L2300/0654 , B01L2300/069 , B01L2300/0819 , C12M1/00 , C12Q1/6825 , G01N21/64 , G01N21/6452
Abstract: An analytical device includes a plate including a first substrate having a front surface and a back surface and a second substrate facing the back surface of the first substrate such that at least one detection space observable from outside is formed between the second substrate and the back surface of the first substrate, and an adapter. The first substrate has a first port having a through hole formed through the front surface and communicated with the detection space for delivering a liquid-containing substance into the detection space, the first substrate has a second port having a through hole formed through the front surface and communicated with the detection space for discharging a liquid-containing substance or a gas from the detection space, and the adapter is attachable to and detachable from the first port and the second port, and the front surface of the first substrate without the adapter is flat.
-
3.
公开(公告)号:US20160333400A1
公开(公告)日:2016-11-17
申请号:US15221143
申请日:2016-07-27
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Yoichi MAKINO , Tomoko Kunitomi
Abstract: A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.
Abstract translation: 生物分子分析试剂盒包括配置成进行酶反应的反应容器,反应容器包括具有容器形状部分和至少设置在容器形状部分的内表面上的低吸附结构部分的基部 低吸附结构部分的吸附率低于在酶反应中成为分析对象的样品中的至少一种和酶反应试剂中的至少一种的吸附速率,其中产生的信号 在反应容器中进行酶反应时,酶反应被配置为检测。
-
公开(公告)号:US20240175074A1
公开(公告)日:2024-05-30
申请号:US18402807
申请日:2024-01-03
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Takumi HIRASE , Yoichi MAKINO , Yosuke HORIUCHI
IPC: C12Q1/6806 , B01L3/00 , C12Q1/686 , G01N33/536
CPC classification number: C12Q1/6806 , B01L3/5027 , C12Q1/686 , G01N33/536 , B01L2200/0689 , B01L2300/0829
Abstract: A method for detecting a target molecule, including supplying a liquid dispersion of a structural substance including a target molecule into a well array such that the structural substance is introduced into wells of the well array, supplying a sealing solution into the well array such that a layer of the sealing solution is formed on the liquid dispersion in the wells, and that the liquid dispersion is sealed in the wells, extracting the target molecule from the structural substance, and detecting the target molecule.
-
公开(公告)号:US20220002645A1
公开(公告)日:2022-01-06
申请号:US17481354
申请日:2021-09-22
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Tomoko KUNITOMI , Yoichi MAKINO
IPC: C12M1/34 , C12M1/00 , G01N21/64 , B01L3/00 , C12Q1/6825
Abstract: A method for filling an analytical device includes providing a plate having a detection space or spaces and ports communicating with the space(s), fitting, into one of the ports, an adapter having a reagent fixed on an inner wall of the adapter such that a first end of the adapter is fitted into the port, and delivering a sample solution into the detection space(s) through the adapter such that the sample solution and reagent fill the detection space(s). The plate includes a first substrate, a second substrate, and a side wall such that the plate has the detection space(s) between the substrates and that the first substrate has the ports, the first and/or second substrates is formed of transparent material such that the detection space is observable from outside the plate, and the ports include a first port for delivering liquid-containing substance, and a second port for discharging the substance.
-
公开(公告)号:US20210394185A1
公开(公告)日:2021-12-23
申请号:US17464114
申请日:2021-09-01
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Keisuke GOTO , Yoichi MAKINO , Yuta SUZUKI
IPC: B01L3/00
Abstract: A microfluidic device including a microwell array having microwells, and a cover member facing the microwell array with a gap between the cover member and the microwell array, and having a flow path formed in the gap. The cover member has a surface facing the microwell array, and the surface has an arithmetic average roughness Ra of 70 nm or less.
-
公开(公告)号:US20190060897A1
公开(公告)日:2019-02-28
申请号:US16173102
申请日:2018-10-29
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Yoichi MAKINO , Akihiro HOSHINO , Tomoko KUNITOMI , Keisuke GOTO , Yosuke HORIUCHI
Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.
-
公开(公告)号:US20210387194A1
公开(公告)日:2021-12-16
申请号:US17458642
申请日:2021-08-27
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Keisuke GOTO , Yoichi MAKINO
Abstract: A microfluidic device including a substrate having at least one droplet holder formed thereon, and a cover member facing the substrate with a space between the cover member and the substrate, and having a flow path formed in the space and connected to the droplet holder. The flow path has a height of more than 0 μm and 30 μm or less.
-
公开(公告)号:US20190062821A1
公开(公告)日:2019-02-28
申请号:US16171630
申请日:2018-10-26
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Masahiro MATSUKAWA , Yoichi MAKINO , Akihiro HOSHINO
IPC: C12Q1/6844 , C12M1/34 , G01N21/64 , G01N33/68 , B01L3/00
Abstract: A reaction container including a transparent base having a first surface having at least one region where recessed portions are formed and recessed from the first surface, and a cover member positioned such that the cover member forms a gap from the first surface inside the region and is welded to the transparent base outside the region. The cover member absorbs infrared light and transmits light having a wavelength within a range of visible light.
-
公开(公告)号:US20220290222A1
公开(公告)日:2022-09-15
申请号:US17751031
申请日:2022-05-23
Applicant: TOPPAN PRINTING CO., LTD.
Inventor: Yoichi MAKINO , Tomoko KUNITOMI
IPC: C12Q1/6858 , B01L3/00 , G01N21/64 , C12Q1/686
Abstract: A biomolecule analysis method may include preparing a reaction container comprising an array of a plurality of micropores in which a wash buffer is filled or preparing the reaction container and feeding the wash buffer into the reaction container including the array of the plurality of micropores. The method may include feeding a reaction reagent to detect a sample, into the plurality of micropores, the sample including a biomolecule. The method may include feeding a sealing solution to seal the plurality of micropores so that the plurality of micropores become a plurality of independent reaction chambers sealed from each other. The method may include causing an isothermal detection reaction in each of the plurality of the independent reaction containers. The method may include detecting a signal amplified by the isothermal detection reaction.
-
-
-
-
-
-
-
-
-