公开(公告)号：US09869852B2

公开(公告)日：2018-01-16

申请号：US14994835

申请日：2016-01-13

Applicant: Thorlabs, Inc.

Inventor： Alex Cable ,  Jörn Wollenzin ,  Ross Johnstone ,  Kirk Gossage ,  Jeffrey S. Brooker ,  Jason Mills ,  James Jiang ,  Dierck Hillmann

IPC: G02B21/24 ,  G02B21/26 ,  G02B5/12 ,  G02B21/08 ,  G01B9/02 ,  G02B7/28 ,  G02B21/00

CPC classification number: G02B21/245 ,  G01B9/02059 ,  G01B9/02063 ,  G01B9/02077 ,  G01B9/0209 ,  G02B7/28 ,  G02B21/0004 ,  G02B21/26

Abstract: Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest in combination with TDI (time delay integration) imaging, confocal and two-photon fluorescence microscopy.

公开(公告)号：US09420207B2

公开(公告)日：2016-08-16

申请号：US14517303

申请日：2014-10-17

Applicant: Thorlabs, Inc.

Inventor： Kirk Gossage ,  Jeffrey Erickson

IPC: H04N5/372 ,  H04N5/353 ,  G02B21/36

CPC classification number: H04N5/37206 ,  G02B21/365 ,  H04N5/353

Abstract: A method for correcting images acquired via an asynchronous triggered acquisition, wherein image acquisition is triggered by a trigger signal based on the motion and/or position of the sample to be imaged by an image sensor, the method including: measurement of the motion and/or position of the sample; determination of an exposure time based on the aforementioned measurement; and correction of an acquired image based on the determined exposure time and a desired exposure time.

Abstract translation: 一种用于校正经由异步触发获取获取的图像的方法，其中基于由图像传感器成像的样本的运动和/或位置由触发信号触发图像采集，所述方法包括：测量运动和/ 或样品的位置; 基于上述测量确定曝光时间; 以及基于所确定的曝光时间和期望的曝光时间来校正所获取的图像。

公开(公告)号：US09989749B2

公开(公告)日：2018-06-05

申请号：US15680962

申请日：2017-08-18

Applicant: Thorlabs, Inc.

Inventor： Alex Cable ,  Jörn Wollenzin ,  Ross Johnstone ,  Kirk Gossage ,  Jeffrey S. Brooker ,  Jason Mills ,  James Jiang ,  Dierck Hillmann

IPC: G02B21/24 ,  G02B7/28 ,  G01B9/02 ,  G02B21/00 ,  G02B21/26

CPC classification number: G02B21/245 ,  G01B9/02059 ,  G01B9/02063 ,  G01B9/02077 ,  G01B9/0209 ,  G02B7/28 ,  G02B21/0004 ,  G02B21/26

Abstract: Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest in combination with TDI (time delay integration) imaging, confocal and two-photon fluorescence microscopy.

公开(公告)号：US20150124119A1

公开(公告)日：2015-05-07

申请号：US14517303

申请日：2014-10-17

Applicant: THORLABS, INC.

Inventor： Kirk Gossage ,  Jeffrey Erickson

IPC: H04N5/353 ,  H04N5/374 ,  H04N5/372

CPC classification number: H04N5/37206 ,  G02B21/365 ,  H04N5/353

Abstract: A method for correcting images acquired via an asynchronous triggered acquisition, wherein image acquisition is triggered by a trigger signal based on the motion and/or position of the sample to be imaged by an image sensor, the method including: measurement of the motion and/or position of the sample; determination of an exposure time based on the aforementioned measurement; and correction of an acquired image based on the determined exposure time and a desired exposure time.

Abstract translation: 一种用于校正经由异步触发获取获取的图像的方法，其中基于由图像传感器成像的样本的运动和/或位置由触发信号触发图像采集，所述方法包括：测量运动和/ 或样品的位置; 基于上述测量确定曝光时间; 以及基于所确定的曝光时间和期望的曝光时间来校正所获取的图像。

公开(公告)号：US20170343787A1

公开(公告)日：2017-11-30

申请号：US15680962

申请日：2017-08-18

Applicant: Thorlabs, Inc.

Inventor： Alex CABLE ,  Jörn Wollenzin ,  Ross Johnstone ,  Kirk Gossage ,  Jeffrey S. Brooker ,  Jason Mills ,  James Jiang ,  Dierck Hillmann

IPC: G02B21/24 ,  G02B7/28 ,  G01B9/02 ,  G02B21/00 ,  G02B21/26

CPC classification number: G02B21/245 ,  G01B9/02059 ,  G01B9/02063 ,  G01B9/02077 ,  G01B9/0209 ,  G02B7/28 ,  G02B21/0004 ,  G02B21/26

Abstract: Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest in combination with TDI (time delay integration) imaging, confocal and two-photon fluorescence microscopy.

公开(公告)号：US09380241B2

公开(公告)日：2016-06-28

申请号：US14520984

申请日：2014-10-22

Applicant: Thorlabs, Inc.

Inventor： Kirk Gossage

IPC: H04N5/374 ,  H04N5/378 ,  H04N5/365 ,  H04N5/372 ,  H04N5/3745

CPC classification number: H04N5/37455 ,  H04N5/3653 ,  H04N5/37213 ,  H04N5/378

Abstract: A method for matching one or more channels to a reference channel in a multiple-channel detector having a digital output, the method including: receiving an analog-to-digital unit (ADU) input value from the multiple-channel detector for each channel; and replacing the ADU input value by an output value corresponding to each channel from a look up table (LUT); wherein the LUT is created by taking a relative intensity ratio of signals from each channel compared to the reference channel over a range of voltage values; calculating a signal value on the detector that generates a specific ADU value across the entire ADU range of the detector; interpolating the relative intensity ratios to obtain ratios corresponding to the calculated signal values for each channel; and dividing the each of the specific ADU value by the corresponding interpolated ratio to obtain the corresponding output value for each channel.

Abstract translation: 一种用于在具有数字输出的多通道检测器中将一个或多个通道匹配到参考通道的方法，所述方法包括：从每个通道的多通道检测器接收模数转换单元（ADU）输入值; 并且从查询表（LUT）将ADU输入值替换为对应于每个通道的输出值; 其中所述LUT是通过在一定范围的电压值下相对于所述参考通道获取来自每个通道的信号的相对强度比而产生的; 计算在检测器上产生在检测器的整个ADU范围内的特定ADU值的信号值; 内插相对强度比以获得对应于每个信道的计算信号值的比率; 并且将每个特定ADU值除以相应的内插比，以获得每个通道的相应输出值。

公开(公告)号：US20150116567A1

公开(公告)日：2015-04-30

申请号：US14520984

申请日：2014-10-22

Applicant: Thorlabs, Inc.

Inventor： Kirk Gossage

IPC: H04N5/372 ,  H04N5/3745

CPC classification number: H04N5/37455 ,  H04N5/3653 ,  H04N5/37213 ,  H04N5/378

Abstract: A method for matching one or more channels to a reference channel in a multiple-channel detector having a digital output, the method including: receiving an analog-to-digital unit (ADU) input value from the multiple-channel detector for each channel; and replacing the ADU input value by an output value corresponding to each channel from a look up table (LUT); wherein the LUT is created by taking a relative intensity ratio of signals from each channel compared to the reference channel over a range of voltage values; calculating a signal value on the detector that generates a specific ADU value across the entire ADU range of the detector; interpolating the relative intensity ratios to obtain ratios corresponding to the calculated signal values for each channel; and dividing the each of the specific ADU value by the corresponding interpolated ratio to obtain the corresponding output value for each channel.

Abstract translation: 一种用于在具有数字输出的多通道检测器中将一个或多个通道匹配到参考通道的方法，所述方法包括：从每个通道的多通道检测器接收模数数字单元（ADU）输入值; 并且从查询表（LUT）将ADU输入值替换为对应于每个通道的输出值; 其中所述LUT是通过在一定范围的电压值下相对于所述参考通道获取来自每个通道的信号的相对强度比而产生的; 计算在检测器上产生在检测器的整个ADU范围内的特定ADU值的信号值; 内插相对强度比以获得对应于每个信道的计算信号值的比率; 并且将每个特定ADU值除以相应的内插比，以获得每个通道的相应输出值。

公开(公告)号：US20160216501A1

公开(公告)日：2016-07-28

申请号：US14994835

申请日：2016-01-13

Applicant: Thorlabs, Inc.

Inventor： Alex CABLE ,  Jörn Wollenzin ,  Ross Johnstone ,  Kirk Gossage ,  Jeffrey S. Brooker ,  Jason Mills ,  James Jiang ,  Dierck Hillmann

IPC: G02B21/24 ,  G02B21/26 ,  G02B5/12 ,  G02B21/08

CPC classification number: G02B21/245 ,  G01B9/02059 ,  G01B9/02063 ,  G01B9/02077 ,  G01B9/0209 ,  G02B7/28 ,  G02B21/0004 ,  G02B21/26

Abstract: Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest in combination with TDI (time delay integration) imaging, confocal and two-photon fluorescence microscopy.

Abstract translation: 公开了使用低相干干涉测量技术来创建用于光学显微镜的自动聚焦装置的几种技术方法。 这些方法允许自动聚焦在紧邻反射表面并在折射材料之后的薄结构，如覆盖滑动，以及将聚焦位置自动调节到样品区域中，而不受邻近表面反射的干扰。 补偿由覆盖样品的材料折射引起的测量偏移。 提出的是一种仪器的技术，允许在低相干干涉测量自动对焦系统中自动交换成像目标，这与TDI（时间延迟积分）成像，共聚焦和双光子荧光显微镜相结合是主要感兴趣的。