公开(公告)号：US20210198633A1

公开(公告)日：2021-07-01

申请号：US17057130

申请日：2019-05-30

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： Maria Cristina NOSTRO ,  Farida SARANGI ,  Roman KORYTNIKOV ,  Emily MCGAUGH ,  Frankie Hoi Chun POON

IPC: C12N5/071

Abstract: Methods and compositions are provided for producing PDX1+/NKX6-1+ pancreatic progenitor cells from a PDX1+ endodermal cell population. The method comprises contacting the endodermal cell population with an EGF component and tankyrase inhibitor that binds to an adenosine subsite of a tankyrase enzyme, to induce the differentiation of at least a portion of the PDX1+ endodermal cell population into PDX1+NKX6-1+ pancreatic progenitor cells.

公开(公告)号：US20190128885A1

公开(公告)日：2019-05-02

申请号：US16043782

申请日：2018-07-24

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： Maria Cristina NOSTRO ,  Thomas KISLINGER ,  Kathryn COGGER ,  Ankit SINHA ,  Farida SARANGI

IPC: G01N33/566 ,  C12N5/071

Abstract: There is described herein a method for enriching/purifying a population of cells for pancreatic progenitor cells, the method comprising: a) providing the population cells, the population comprising pancreatic progenitor cells; and b) performing at least one of steps (i)-(v): (i) selecting for cells from the population that express at least one protein listed in cluster 2; (ii) selecting for cells from the population that express at least one protein listed in cluster 5; (iii) deselecting for cells from the population that express at least one protein listed in cluster 1; (iv) deselecting for cells from the population that express at least one protein listed in cluster 3; and (v) deselecting for cells from the population that express at least one protein listed in cluster 6.