公开(公告)号：US20250136942A1

公开(公告)日：2025-05-01

申请号：US18932104

申请日：2024-10-30

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Zhipeng LU ,  Minjie ZHANG ,  Kongpan LI ,  Jianhui BAI

IPC: C12N5/077 ,  A61K35/34 ,  C12N9/22 ,  C12N15/113

Abstract: The disclosure provides methods of promoting stem cell differentiation into mesoderm and endoderm cell lineages comprising, for example, contacting a stem cell with an agent that reduces or eliminates gene activity of one or more endogenous small nuclear ribonucleic acid (snoRNA) molecules, wherein the snoRNA molecules comprise SNORD97 and/or SNORD133. Also provided are isolated cardiomyocytes and compositions of the same, wherein the cardiomyocytes are produced using a method described herein.