公开(公告)号：US12203921B2

公开(公告)日：2025-01-21

申请号：US18528948

申请日：2023-12-05

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  B01L3/00 ,  C12Q1/6869 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US11474094B2

公开(公告)日：2022-10-18

申请号：US16215139

申请日：2018-12-10

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N21/65 ,  B01L3/00 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US20220119867A1

公开(公告)日：2022-04-21

申请号：US17563718

申请日：2021-12-28

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Jeremy Scott Edwards

IPC: C12Q1/689 ,  C12Q1/6853

Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, the method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

公开(公告)号：US11248271B2

公开(公告)日：2022-02-15

申请号：US16369372

申请日：2019-03-29

Applicant: UNM Rainforest Innovations

Inventor： Jeremy Scott Edwards

IPC: C12Q1/689 ,  C12Q1/6853

Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, the method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

公开(公告)号：US20210180137A1

公开(公告)日：2021-06-17

申请号：US17263757

申请日：2019-05-23

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Jeremy Scott Edwards ,  Paul Szauter ,  Robert B. Sinclair ,  Kirsten Dimmler

IPC: C12Q1/6883

Abstract: This disclosure describes detecting four genetically distinct kinds of inherited myopathy in horses, referred to as Polysaccharide Storage Myopathy type 2 (PSSM2), or in some cases as Myofibrillar Myopathy (MFM).