公开(公告)号：US10976299B1

公开(公告)日：2021-04-13

申请号：US15944226

申请日：2018-04-03

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Steven R. J. Brueck ,  Yuliya Kuznetsova ,  Alexander Neumann

IPC: B01D69/04 ,  B81C1/00 ,  G01N33/487 ,  G03F7/20 ,  C12Q1/6869 ,  G01N27/447

Abstract: In accordance with the disclosure, a method of forming a nanochannel is provided. The method includes depositing a photosensitive film stack over a substrate; forming a pattern on the film stack using interferometric lithography; depositing a plurality of silica nanoparticles to form a structure over the pattern; removing the pattern while retaining the structure formed by the plurality of silica nanoparticles, wherein the structure comprises one or more enclosed nanochannels, wherein each of the one or more nanochannels comprise one or more sidewalls and a roof; and partially sealing the roof of one or more nanochannels, wherein the roof comprises no more than one unsealed nanochannel per squared micron.

公开(公告)号：US12203921B2

公开(公告)日：2025-01-21

申请号：US18528948

申请日：2023-12-05

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  B01L3/00 ,  C12Q1/6869 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US11474094B2

公开(公告)日：2022-10-18

申请号：US16215139

申请日：2018-12-10

Applicant: UNM RAINFOREST INNOVATIONS

Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N21/65 ,  B01L3/00 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.