公开(公告)号：WO2015176026A1

公开(公告)日：2015-11-19

申请号：PCT/US2015/031227

申请日：2015-05-15

Applicant: INVISTA NORTH AMERICA S.Á.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P13/04 ,  C12N15/52

CPC classification number: C12P13/001 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P13/005 ,  C12P13/04 ,  C12Y102/99006 ,  C12Y103/01031 ,  C12Y402/01 ,  C12Y403/01

Abstract: This document describes biochemical pathways for producing 2- aminopimelate from 2,6-diaminopimelate, and methods for converting 2- aminopimelate to one or more of adipic acid, adipate semialdehyde, caprolactam, 6- aminohexanoic acid, 6-hexanoic acid, hexamethylenediamine, or 1,6-hexanediol by decarboxylating 2-aminopimelate into a six carbon chain aliphatic backbone and enzymatically forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in the backbone.

Abstract translation: 本文件描述了从2,6-二氨基庚二酸酯生产2-氨基庚二酸的生物化学途径，以及将2-氨基庚二酸转化成己二酸，己二酸半醛，己内酰胺，6-氨基己酸，6-己酸，六亚甲基二胺或 通过将2-氨基庚二酸脱羧成六碳链脂肪族主链并在骨架中酶促形成由羧基，胺或羟基组成的一个或两个末端官能团。

公开(公告)号：WO2018005770A8

公开(公告)日：2018-01-04

申请号：PCT/US2017/039954

申请日：2017-06-29

Applicant: INVISTA NORTH AMERICA S.Á.R.L.

Inventor： CHOKKATHUKALAM, Achuthanunni ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane ,  SURAE, Satnam ,  TIBBLES, Katherine Louise

IPC: C12N15/52 ,  C12N9/04

Abstract: The present disclosure relates to methods for more efficiently recycling reduced electron carriers in a hydrogen-oxidizing microorganism with an operable Calvin-Benson cycle; synthetic carbon fixation pathways that recycle reduced electron carriers more efficiently than the Calvin-Benson cycle, such as methods for enzymatically converting carbon dioxide to formate and assimilating the resulting formate into central carbon metabolism; methods for producing biochemical products; and recombinant hosts utilizing one or more synthetic carbon fixation pathways.

公开(公告)号：WO2018005770A3

公开(公告)日：2018-01-04

申请号：PCT/US2017/039954

申请日：2017-06-29

Applicant: INVISTA NORTH AMERICA S.Á.R.L.

Inventor： CHOKKATHUKALAM, Achuthanunni ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane ,  SURAE, Satnam ,  TIBBLES, Katherine Louise

IPC: C12N1/13 ,  C12N1/21 ,  C12N5/10 ,  C12N9/88 ,  C12N15/63 ,  C12P7/16

Abstract: The present disclosure relates to methods for more efficiently recycling reduced electron carriers in a hydrogen-oxidizing microorganism with an operable Calvin-Benson cycle; synthetic carbon fixation pathways that recycle reduced electron carriers more efficiently than the Calvin-Benson cycle, such as methods for enzymatically converting carbon dioxide to formate and assimilating the resulting formate into central carbon metabolism; methods for producing biochemical products; and recombinant hosts utilizing one or more synthetic carbon fixation pathways.

公开(公告)号：WO2016175901A2

公开(公告)日：2016-11-03

申请号：PCT/US2015/067099

申请日：2015-12-21

Applicant: INVISTA NORTH AMERICA S.Á.R.L. ,  INVISTA TECHNOLOGIES S.A.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P7/26

CPC classification number: C12P19/32 ,  C07H1/00 ,  C07H19/207 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/24 ,  C12P7/26 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y203/01

Abstract: This document describes materials and methods for, for example, producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a β-ketothiolase or synthase and an alcohol O-acetyltransferase to form a 6-acetyloxy-3-oxohexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

Abstract translation: 该文献描述了例如使用β-酮硫解酶或合酶和醇O-乙酰转移酶产生6-羟基己酸以形成6-乙酰氧基-3-氧代己酰-CoA中间体的材料和方法。 本文件描述了使用β-酮硫解酶或合酶和醇O-乙酰转移酶生成6-羟基己酸的生物化学途径以形成6-乙酰氧基-3-氧代己酰-CoA中间体。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文献还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。

公开(公告)号：WO2016085811A1

公开(公告)日：2016-06-02

申请号：PCT/US2015/061861

申请日：2015-11-20

Applicant: INVISTA NORTH AMERICA S.Á.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane

IPC: C12P7/42 ,  C12P7/64 ,  C12P13/00 ,  C12N9/02 ,  C12N9/18

CPC classification number: C12P7/6409 ,  C07C47/12 ,  C07C47/19 ,  C07C55/16 ,  C07C59/01 ,  C07C225/06 ,  C07C229/08 ,  C07C233/31 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/16 ,  C12N9/18 ,  C12P7/42 ,  C12P13/001 ,  C12P13/005 ,  C12Y114/14001 ,  C12Y206/01 ,  C12Y301/01003

Abstract: This document describes biochemical pathways for producing 7-hydroxyheptanoic acid using a polypeptide having monooxygenase activity to form a 8-hydroxynonanoate intermediate, which can be converted to 7-hydroxyheptanoate using a polypeptide having monooxygenase activity, a polypeptide having secondary alcohol dehydrogenase activity, and a polypeptide having esterase activity. 7-hydroxyheptanoic acid can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7 heptanediol. This document also describes recombinant hosts producing 7-hydroxyheptanoic acid as well as pimelic acid, 7-aminoheptanoic acid, heptamethylenediamine and 1,7 heptanediol.

Abstract translation: 本文件描述了使用具有单加氧酶活性的多肽形成8-羟基壬酸酯中间体以产生7-羟基庚酸的生物化学途径，其可以使用具有单加氧酶活性的多肽，具有仲醇脱氢酶活性的多肽转化为7-羟基庚酸酯， 具有酯酶活性的多肽。 7-羟基庚酸可以酶促转化成庚二酸，7-氨基庚酸，七亚甲基二胺或1,7庚二醇。 该文献还描述了产生7-羟基庚酸以及庚二酸，7-氨基庚酸，七亚甲基二胺和1,7庚二醇的重组宿主。

公开(公告)号：WO2016077800A1

公开(公告)日：2016-05-19

申请号：PCT/US2015/060747

申请日：2015-11-13

Applicant: INVISTA NORTH AMERICA S.Á.R.L. ,  INVISTA TECHNOLOGIES S.A R.L.

Inventor： BOTES, Adriana, Leonora ,  CONRADIE, Alex Van Eck ,  KADI, Nadia

IPC: C12P7/42 ,  C12P13/00 ,  C12P7/18 ,  C12P7/44 ,  C12P17/10 ,  C12N9/10

CPC classification number: C12P19/32 ,  C07C47/12 ,  C07C47/19 ,  C07C225/06 ,  C07C229/08 ,  C07C233/31 ,  C07F9/65616 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y101/01036 ,  C12Y101/011 ,  C12Y101/01157 ,  C12Y102/99006 ,  C12Y103/01008 ,  C12Y103/01038 ,  C12Y103/01044 ,  C12Y203/01016 ,  C12Y203/01174 ,  C12Y206/01 ,  C12Y402/01017 ,  C12Y402/01119

Abstract: This document describes biochemical pathways for producing 6-hydroxyhexanoic acid using a polypeptide having ß-ketothiolase activity to form a 3-oxo-6-hydroxyhexanoyl-CoA intermediate. 6-hydroxyhexanoic acid can be enzymatically converted to adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol. This document also describes recombinant hosts producing 6-hydroxyhexanoic acid as well as adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine and 1,6-hexanediol.

Abstract translation: 本文件描述了使用具有β-酮硫羰酸酶活性的多肽形成3-氧代-6-羟基己酰-CoA中间体的生产6-羟基己酸的生化途径。 6-羟基己酸可以酶促转化为己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺或1,6-己二醇。 该文献还描述了生产6-羟基己酸以及己二酸，己内酰胺，6-氨基己酸，六亚甲基二胺和1,6-己二醇的重组宿主。

公开(公告)号：WO2018005770A2

公开(公告)日：2018-01-04

申请号：PCT/US2017/039954

申请日：2017-06-29

Applicant: INVISTA NORTH AMERICA S.Á.R.L.

Inventor： CHOKKATHUKALAM, Achuthanunni ,  CONRADIE, Alex Van Eck ,  HADDOUCHE, Ramdane ,  SURAE, Satnam ,  TIBBLES, Katherine Louise

IPC: C12N15/52 ,  C12N9/04

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/40 ,  C12Y101/01027 ,  C12Y101/99007 ,  C12Y102/01002 ,  C12Y102/0101 ,  C12Y102/01043 ,  C12Y103/01084 ,  C12Y203/01054 ,  C12Y203/03005 ,  C12Y208/03 ,  C12Y401/01031 ,  C12Y403/01006 ,  C12Y403/01019 ,  C12Y404/01001 ,  C12Y503/01027 ,  C12Y602/01036 ,  Y02P20/134

Abstract: The present disclosure relates to methods for more efficiently recycling reduced electron carriers in a hydrogen-oxidizing microorganism with an operable Calvin-Benson cycle; synthetic carbon fixation pathways that recycle reduced electron carriers more efficiently than the Calvin-Benson cycle, such as methods for enzymatically converting carbon dioxide to formate and assimilating the resulting formate into central carbon metabolism; methods for producing biochemical products; and recombinant hosts utilizing one or more synthetic carbon fixation pathways.

Abstract translation: 本公开涉及用可操作的卡尔文 - 本森循环更有效地再循环氢氧化微生物中的还原电子载体的方法; 比Calvin-Benson循环更有效地循环还原电子载体的合成碳固定途径，例如将二氧化碳酶促转化为甲酸并将所得甲酸同化为中心碳代谢的方法; 生产生化产品的方法; 和利用一种或多种合成碳固定途径的重组宿主。

公开(公告)号：WO2017111904A2

公开(公告)日：2017-06-29

申请号：PCT/US2015/067090

申请日：2015-12-21

Applicant: INVISTA NORTH AMERICA S.Á.R.L. ,  INVISTA TECHNOLOGIES S.Á.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

CPC classification number: C08L77/12 ,  C07B2200/07 ,  C07C209/68 ,  C07C213/02 ,  C07C221/00 ,  C07C227/18 ,  C07C227/32 ,  C08G69/08 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42 ,  C12P13/001 ,  C12Y101/01061 ,  C12Y102/99006 ,  C12Y206/01018 ,  C12Y401/01015 ,  C12Y401/01071 ,  C07C229/24 ,  C07C229/12 ,  C07C223/02 ,  C07C215/08 ,  C07C211/09

Abstract: This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.

Abstract translation:

本文描述了在C5骨架通过形成一个或两个的末端官能团产生4-羟基丁酸酯，4-氨基丁酸酯，腐胺或1,4-丁二醇的生化途径，由胺或羟基基团的， 底物如2-酮戊二酸或L-谷氨酸。

公开(公告)号：WO2015175698A1

公开(公告)日：2015-11-19

申请号：PCT/US2015/030627

申请日：2015-05-13

Applicant: INVISTA NORTH AMERICA S.Á.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P7/40 ,  C12N15/63 ,  C12P7/44 ,  C12P7/62 ,  C12P17/10

CPC classification number: C12P13/001 ,  C08G83/00 ,  C12N15/52 ,  C12P7/04 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/625 ,  C12P17/10 ,  C12Y102/99006 ,  C12Y206/01

Abstract: This document describes biochemical pathways for producing adipyl-[acp] and either hexanoic acid or acetic acid from a long chain acyl-[acp] such as dodecanoyl-[acp] or octanoyl-[acp] using a polypeptide having pimeloyl-[acp] synthase activity and biochemical pathways for converting adipyl-[acp] and/or hexanoic acid to one of more of adipic acid, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine, caprolactam, and 1,6-hexanediol.

Abstract translation: 该文献描述了使用具有双甲酰基 - [acp]的多肽从长链酰基 - [acp]如十二酰基 - [acp]或辛酰基 - [acp]生产己二酰 - [acp]和己酸或乙酸的生物化学途径 将己二酰 - [acp]和/或己酸转化为己二酸，6-氨基己酸，6-羟基己酸，六亚甲基二胺，己内酰胺和1,6-己二醇中的一种以上的合成酶活性和生化途径。

公开(公告)号：WO2017111904A3

公开(公告)日：2017-06-29

申请号：PCT/US2015/067090

申请日：2015-12-21

Applicant: INVISTA NORTH AMERICA S.Á.R.L. ,  INVISTA TECHNOLOGIES S.Á.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck

IPC: C12P7/18 ,  C12P7/42 ,  C12P13/00 ,  C12N15/52

Abstract: This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.