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公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract translation: 提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。 免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。 酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。
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公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract translation: 提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。 免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。 一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。
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3.发明公开동물의 면역능을 증강시키는 노란 민들레 열탕 추출물, 그제법 및 용도 无效用于改善动物免疫力的UM UM AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME
公开(公告)号：KR1020090021611A
公开(公告)日：2009-03-04
申请号：KR1020070086251
申请日：2007-08-27
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/288 , A61P37/02
Abstract: Provided is an immunostimulant using dandelion extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunostimulant using dandelion extract comprises dandelion extract obtained by a hot water process as an active ingredient. A method for preparing the immunostimulant comprises a steps of extracting the dandelion extract at a temperature of 90~100°C by hot distilled water. The dandelion extract can be used independently or with additives which are generally used.
Abstract translation: 提供使用蒲公英提取物的免疫刺激剂，其改善对病原体的非特异性防御的功能并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。 使用蒲公英提取物的免疫刺激剂包含通过热水法获得的蒲公英提取物作为活性成分。 制备免疫刺激剂的方法包括以下步骤：通过热蒸馏水在90〜100℃的温度下提取蒲公英提取物。 蒲公英提取物可以单独使用或与通常使用的添加剂一起使用。
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公开(公告)号：KR100951055B1
公开(公告)日：2010-04-06
申请号：KR1020070093644
申请日：2007-09-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/344 , A61P37/02
Abstract: 본 발명은 동물의 면역능을 증강시키는 더덕 추출물 및 그 제조방법과 용도에 관한 것이다. 보다 상세하게는 더덕줄기 및 잎을 음지에서 건조시켜 마쇄하여 열탕용 봉지에 넣고 증류수 중에 가열하여 1차 추출한 후, 추출물은 별도로 분리하고 잔류물에 재차 증류수를 넣고 열탕하여 2차 추출하여 추출물을 얻는 단계와, 1차 및 2차 추출물을 혼합하여 추출물에 2배량의 에탄올을 가하여 4∼6℃ 냉장실에서 24시간 정치시켜 침전된 다당체를 원심분리하는 단계와, 분리된 다당체를 순수 에탄올로 원심 세척한 후 35∼38℃ 항온실에서 건조하여 증류수로 1mg/ml 농도로 용해시켜 세균여과지로 여과하여 원액을 얻는 단계로 구성된다.
본 발명의 더덕추출물은 동물의 주요 면역세포인 호중구의 각종 활성과 싸이토카인 생성을 증강시키고 병원성세균의 공격접종에 대한 비특이적인 방어능을 증진시키는 효과가 있으므로 동물용 의약품으로써 면역증강제. 백신보좌제 첨가물 그리고 보조치료제 등으로 광범위하게 활용될 수 있을 것이다.-
公开(公告)号：KR100918098B1
公开(公告)日：2009-09-24
申请号：KR1020070077854
申请日：2007-08-02
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/02 , A61P37/02 , A61K39/085
Abstract: 본 발명은 스트렙토마이세스 알부스 용균 추출물을 포함하는 백신 보좌제에 관한 것으로, 보다 자세히는 스트렙토마이세스 알부스(Streptomyces albus) 용균 추출물을 이용하여 동물의 면역 능력을 향상시키는 백신 보좌제에 관한 것으로, 동물의 주요 면역세포인 호중구 및 림프구 등의 활성을 증가시켜 병원성 세균의 공격에 대하여 면역 기능을 증진시키는 스트렙토마이세스 알부스(Streptomyces Albus) 용균 추출물을 포함하는 백신 보좌제를 제공하는 것을 목적으로 한다.
본 발명은 이와 같은 스트렙토마이세스 알부스로부터 추출한 용균 추출물을 이용하여 질병 방어에 주요한 역할을 담당하고 있는 각종 면역세포들의 기능을 증강시켜 질병 감염에 대한 특이 및 비특이적 저항성을 높여서, 질병으로부터 동물을 보호할 수 있는 효과를 제공한다.
스트렙토마이세스 알부스, 용균 추출물, 면역-
Patent Agency Ranking
公开(公告)号：KR1020090028213A
公开(公告)日：2009-03-18
申请号：KR1020070093644
申请日：2007-09-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/344 , A61P37/02
Abstract: A manufacturing method of extract of codonopsis lanceolata is provided to increase immunomodulation enhancement of pathogenic bacteria, to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of extract of codonopsis lanceolata comprises steps of: obtaining the first extracts and residue by heating powder of leaf and stem of the dried Condonopsis lanceolata Trautv into distilled water, separating the first extracts separately, putting it into residue, heating it obtaining the second extraction extracts; mixing the first extracts of the obtained Condonopsis lanceolata Trautv stem and leaf and the second extracts of Condonopsis lanceolata Trautv stem and leaf, putting ethanol into the mixing extract, obtaining precipitated polysaccharide by stationing the obtained solution and centrifuging the precipitated polysaccharide; and center-rinsing the separated polysaccharide to the ethanol, drying the washed material, dissolving dried material with distilled water, filtering the obtained solution and obtaining undiluted solution.
Abstract translation: 提供密码子提取物的制造方法，以增加病原菌的免疫调节增强作用，有效地用于预防和治疗动物有效疾病的免疫刺激剂，疫苗的辅助改善效果和辅助治疗，增强治疗功效。 密码子提取物的制造方法包括以下步骤：通过将干燥的马铃薯干燥杆菌的叶和茎的粉末加热到蒸馏水中获得第一提取物和残留物，分离第一提取物，将其放入残渣中，加热得到 第二提取提取物; 混合所得到的Condonopsis lanceolata Trautv茎和叶的第一提取物和Condonopsis lanceolata Trautv茎和叶的第二提取物，将乙醇加入到混合提取物中，通过沉淀所得溶液并离心沉淀的多糖得到沉淀的多糖; 将分离的多糖中心漂洗至乙醇，干燥洗涤过的物质，用蒸馏水溶解干燥物，过滤得到的溶液，得到未稀释溶液。
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公开(公告)号：KR1020090028212A
公开(公告)日：2009-03-18
申请号：KR1020070093643
申请日：2007-09-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K35/648
CPC classification number: A61K35/648
Abstract: A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal is provided to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal comprises steps of: put the Scolopendra subspinipes powder into distilled water; obtaining extract by heating obtained solution, filtering obtained extract using filter paper firstly, filtering filtered material using filter paper secondly and sterilizing filtered material.
Abstract translation: 提供了提供动物免疫原性的Scolopendra subspinipes提取物的制造方法，可用作有效预防和治疗动物疾病的免疫刺激剂，疫苗的辅助改善效果和辅助治疗，增强治疗功效。 Scolopendra subspinipes提取物制备动物免疫原性的方法包括以下步骤：将Scolopendra subspinipes粉末放入蒸馏水中; 通过加热获得的溶液获得提取物，首先使用滤纸过滤获得的提取物，然后用滤纸过滤过滤材料并对过滤材料进行灭菌。
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公开(公告)号：KR1020090013600A
公开(公告)日：2009-02-05
申请号：KR1020070077855
申请日：2007-08-02
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immunostimulant containing the lystic extract of Candida sake is provided to increase activity of neutrophil and lymphocyte as major immune cells of animals, thereby protecting animals from diseases. The immunostimulant contains the lystic extract of Candida sake which is prepared by cultivating Candida sake in yeast malt extract agar broth at 30 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Candida sake suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
Abstract translation: 提供含有念珠菌精液提取物的免疫刺激剂，以增加嗜中性粒细胞和淋巴细胞作为动物主要免疫细胞的活性，从而保护动物免受疾病。 免疫刺激剂含有通过在30℃下在酵母麦芽提取物琼脂培养液中培育念珠菌精油而制备的念珠菌清酒。 培养72小时，将培养液以3000Xg离心30分钟并收集，用pH7.2的磷酸盐缓冲盐水（PBS）冲洗收集的沉淀物，并将漂洗的沉淀悬浮于PBS中，加入5％含有50mM Tris -HCl，0.15M NaCl和4％Triton x-114作为增溶剂加入到念珠菌悬浮液中，在室温下搅拌混合物2-3天以溶解链霉菌，并将溶液的pH调节至6.5-7.2 ，并干燥恒温室中的溶液。
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公开(公告)号：KR1020090012575A
公开(公告)日：2009-02-04
申请号：KR1020070076507
申请日：2007-07-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K31/70 , A61K36/48 , A61K36/185 , A61P37/02
CPC classification number: A61K38/168 , Y10S514/885
Abstract: Glycine max (L.) Merrill lectin is provided to reinforce immunogenicity of animals, to improve effect of an immunoadjuvant and a vaccine preventing and treating disease of the animals efficiently and to increase defence ability aspecific to attack inoculation of pathogenic bacteria. An immunoadjuvant comprises Glycine max (L.) Merrill lectin as an active ingredient. Additionally, the Glycine max (L.) Merrill lectin is used for a vaccine throne and an adjuvant treatment as the active ingredient.
Abstract translation: 提供大豆（L.）美林凝集素以增强动物的免疫原性，提高免疫佐剂的效果和预防和治疗动物疾病的疫苗，并提高对病原菌接种的防御能力。 免疫佐剂包含甘氨酸（L.）美林凝集素作为活性成分。 另外，甘氨酸（L.）美林凝集素用作疫苗王位和辅助治疗作为活性成分。
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公开(公告)号：KR1020080032809A
公开(公告)日：2008-04-16
申请号：KR1020060098780
申请日：2006-10-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: Y02A50/473 , A61K35/74 , A61K36/06 , C12N1/16 , C12N1/20
Abstract: A method for preparing a cell lysis composition of bacterial and yeast cell wall is provided to improve yield of cell wall, and selectively employ antigen substances and immune-enhancing substances in the cell wall by using biochemical and chemical treatment, so that the composition is useful as vaccine antigen, heterologous delivery system, immune enhancer, vaccine assistant and complex feed additive. A method for preparing a cell lysis composition of bacterial and yeast cell wall comprises the steps of: culturing gram positive bacteria, gram negative bacteria and yeast; treating the cultured gram positive bacteria, gram negative bacteria and yeast biochemically and chemically by using transparent outer membrane extraction, TNT(Tris-HCl, sodium chloride, Triton X-114) method, NaOH-EDTA(sodium hydroxide-(ethylene dinitrilo) tetraacetic acid), TMC(Tris-HCl, magnesium chloride, calcium chloride) method or TET(Tris-HCl, EDTA, Triton X-100) method; and extracting useful ingredients of cell wall from the treated gram positive bacteria, gram negative bacteria and yeast, wherein the gram negative bacteria include Escherichia coli 055 and Bordetella pertussis; the gram positive bacteria include Staphylococcus aureus Cowan 1 and Erysipelothrix rhusiopathiae T2; and the yeast is Saccharomyces cerevisiae or Candida albicans. Further, the useful ingredients of cell wall are selected from a group consisting of a transparent cell outer membrane, a cell wall and bacteriolysant.
Abstract translation: 提供细菌和酵母细胞壁细胞裂解组合物的制备方法，以提高细胞壁的产量，并通过生物化学和化学处理选择性地在细胞壁中使用抗原物质和免疫增强物质，使组合物有用 作为疫苗抗原，异源递送系统，免疫增强剂，疫苗助剂和复合饲料添加剂。 制备细菌和酵母细胞壁的细胞裂解组合物的方法包括以下步骤：培养革兰氏阳性菌，革兰氏阴性细菌和酵母; 通过使用透明的外膜提取，TNT（Tris-HCl，氯化钠，Triton X-114）方法，NaOH-EDTA（氢氧化钠 - （亚乙基二硝基）四乙酸）处理培养的革兰氏阳性细菌，革兰氏阴性细菌和酵母生物化学和化学 酸），TMC（Tris-HCl，氯化镁，氯化钙）法或TET（Tris-HCl，EDTA，Triton X-100）法; 并从经处理的革兰氏阳性细菌，革兰氏阴性细菌和酵母提取细胞壁的有用成分，其中革兰氏阴性细菌包括大肠杆菌055和百日咳博德特氏菌; 革兰氏阳性细菌包括金黄色葡萄球菌Cowan 1和Erysipelothrix rhusiopathiae T2; 酵母是酿酒酵母或白色念珠菌。 此外，细胞壁的有用成分选自透明细胞外膜，细胞壁和细菌悬液。
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