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公开(公告)号：WO2011016632A2
公开(公告)日：2011-02-10
申请号：PCT/KR2010/004754
申请日：2010-07-21
IPC: C12N15/29 , C07K14/415 , C12N15/82 , A01H1/00
CPC classification number: C07K14/415 , C12N15/8294
Abstract: 본 발명은 벼 유래의 식물의 세포막 수소펌프 활성화에 관여하는 옥신 수용체 단백질, 상기 단백질을 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 숙주세포, 상기 재조합 식물 발현 벡터로 식물체를 형질전환시켜 식물체의 형질을 개선하는 방법, 상기 재조합 식물 발현 벡터로 형질전환되어 형질이 개선된 식물체 및 이의 종자, 및 상기 유전자를 포함하는 식물체의 형질을 개선하기 위한 조성물에 관한 것이다.
Abstract translation: 本发明涉及：参与水稻植物的细胞膜氢泵活化的生长素受体蛋白; 编码蛋白质的基因; 包含该基因的重组载体; 使用重组载体遗传转化的宿主细胞; 使用重组植物表达载体通过转基因植物来改善植物群的性状的方法; 通过使用重组植物表达载体的遗传转化，其性状已经改善的植物群及其种子; 以及用于改善包含该基因的植物菌群性状的组合物。
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公开(公告)号：KR1020140147519A
公开(公告)日：2014-12-30
申请号：KR1020130070929
申请日：2013-06-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/689 , C12N15/11 , C12Q1/6844 , C12Q1/6895
Abstract: The present invention relates to a Rhizoctonia solani AROM (RsAROM) gene-specific primer and to a method for detecting sheath blight by using the same, which can be used as a molecular biological method for diagnosing sheath blight by using a primer that can specifically detect RsAROM gene existing in a sheath blight genome and a probe to objectively quantify the development of sheath blight.
Abstract translation: 本发明涉及丝核菌属AROM（RsAROM）基因特异性引物及其用于检测皮肤枯萎病的方法，其可以用作通过使用可特异性检测的引物来诊断纹枯病的分子生物学方法 存在于纹枯病基因组中的RsAROM基因，以及客观量化纹枯病发展的探针。
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公开(公告)号：KR1020130070297A
公开(公告)日：2013-06-27
申请号：KR1020110137541
申请日：2011-12-19
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A promoter and a transformant which is prepared using a recombinant containing the same is provided to be expressed in a scutellum or scutellum-derived meristem of Oryza sativa seeds, and to regulate seed-specific material production, quiescence, germination, and growth. CONSTITUTION: A promoter for target protein-specific expression in a scutellum or a scutellum-derived meristem of Oryza sative seeds contains a base sequence in sequence number 1. A recombinant expression vector contains the promoter and a structural gene which encodes a target protein and is operatively linked. A transformant is prepared by transformation by the vector and is Agrobacterium tumefaciens. A transgenic plant is transformed by the vector or the transformant. The transgenic plant is Oryza sativa.
Abstract translation: 目的：提供使用含有该重组体的重组体制备的启动子和转化体，以在水稻种子的盾片或盾片衍生的分生组织中表达，并调节种子特异性材料产生，静止，发芽和生长。 构成：在水稻或盾片衍生的水稻种子分生组织中靶蛋白特异性表达的启动子含有序列号1中的碱基序列。重组表达载体含有启动子和编码靶蛋白的结构基因， 可操作地连接。 通过载体转化并根癌土壤杆菌制备转化体。 通过载体或转化体转化转基因植物。 转基因植物是水稻。
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公开(公告)号：KR1020120069375A
公开(公告)日：2012-06-28
申请号：KR1020100130894
申请日：2010-12-20
Applicant: 대한민국(농촌진흥청장)
IPC: A01H5/00 , C07K14/415 , C12N15/29 , C12N15/113
CPC classification number: C07K14/415 , C12N15/8205 , C12N15/8216 , C12N15/8225 , C12N15/8261
Abstract: PURPOSE: A method for controlling growth or shape of a plant using a gene isolated from Oriza sativa is provided to express a transformant of OsTCP6 gene in the plant and to enhance crop productivity. CONSTITUTION: A protein which regulates growth or shape of a plant has an amino acid sequence of sequence number 2. A gene encoding the protein has a base sequence of sequence number 1. The growth or shape is regulated by controlling intracellular protein level. The intracellular protein level is controlled by introducing the gene into plant cells or transforming the plant with the gene. The gene is PCNA, cyclinD3, cyclinB1, cdc2b or expansin. A composition for controlling growth or shape of the plant contains the protein or transformant.
Abstract translation: 目的：提供使用从东方gene分离的基因来控制植物生长或形状的方法，以表达植物中OsTCP6基因的转化体并提高作物生产力。 构成：调节植物生长或形状的蛋白质具有序列号2的氨基酸序列。编码蛋白质的基因具有序列号1的碱基序列。通过控制细胞内蛋白质水平调节生长或形状。 通过将基因导入植物细胞或用该基因转化植物来控制细胞内蛋白质水平。 该基因是PCNA，cyclinD3，cyclinB1，cdc2b或扩展蛋白。 用于控制植物生长或形状的组合物含有蛋白质或转化体。
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公开(公告)号：KR1020110013871A
公开(公告)日：2011-02-10
申请号：KR1020090071553
申请日：2009-08-04
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29 , C07K14/415 , C12N15/82 , A01H1/00
CPC classification number: C07K14/415 , C12N15/8294
Abstract: PURPOSE: Auxin receptor gene ABP57 relating to the cell membrane hydrogen pump activation of rice-derived plant is provided to develop novel crops and a crop protective agent. CONSTITUTION: Auxin receptor protein ABP57 relating to the cell membrane hydrogen pump activation of rice-derived plant has an amino acid sequence selected from sequence numbers 1-5. A gene encoding the ABP57 has an amino acid sequence of sequence numbers 6-10. Plant characteristics are improved by transforming a host cell with a recombinant protein. A composition for improving plant characteristics contains the gene encoding ABP57.
Abstract translation: 目的：提供与水稻植物细胞膜氢泵活化有关的生物素受体基因ABP57，用于开发新型作物和作物保护剂。 构成：涉及水稻源植物的细胞膜氢泵活化的生物素受体蛋白质ABP57具有选自序列号1-5的氨基酸序列。 编码ABP57的基因具有序列号6-10的氨基酸序列。 通过用重组蛋白转化宿主细胞来改善植物特征。 用于改善植物特性的组合物包含编码ABP57的基因。
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Patent Agency Ranking6.发明授权내재 라이보좀 진입 부위 배열을 포함하는 베타카로틴생합성 유도 다중 발현 재조합 유전자 피아이씨, 이를포함하는 발현 벡터 및 그 형질전환 세포 有权重组PIC基因，包括用于β-胡萝卜素生物合成的十字花科感染烟草花叶病毒的内部核糖体进入位点序列，包含其的表达载体和转化体细胞
公开(公告)号：KR100987013B1
公开(公告)日：2010-10-11
申请号：KR1020080010138
申请日：2008-01-31
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 내재 라이보좀 진입 부위(internal ribosome entry site) 배열을 이용하여 식물 대사공학에 유용한 유전자 다중발현 기술을 확립하고 그 기술을 이용한 베타카로틴 생합성 다중 발현 재조합 유전자, 그 발현 벡터 및 그 형질전환 벼 식물체의 개발에 관한 것이다.
본 발명에 의하면 베타카로틴(프로비타민 A)이 축적되는 형질전환 쌀을 얻을 수 있다.
내재 라이보좀 진입 부위, 베타카로틴, 다중 발현, 발현 벡터, 형질전환-
公开(公告)号：KR1020150001083A
公开(公告)日：2015-01-06
申请号：KR1020130073737
申请日：2013-06-26
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/04 , C12Q1/6848 , C12Q1/689
Abstract: 본 발명은 검은무늬병을 유발하는 검은무늬병원균
Alternaria
brassicicola 의 병원성 관련 유전자인 AbSCD1의 특이적이고 정량적인 검출을 위한 프라이머 및 프로브에 관한 것으로, 더욱 구체적으로는 상기 프라이머 및 프로브를 사용한 실시간 PCR 방법은 검은무늬병원균을 특이적 및 정량적으로 검출 가능하므로, 검은무늬병의 진단 및 병의 진전에 대한 객관적 정량화에 이용될 수 있다.Abstract translation: 本发明涉及用于特异性检测与引起黑点的链格孢（Alternaria brassicicola）致病基因相关的定量AbSCD1的引物和探针。 更具体地，使用引物和探针的实时聚合酶链反应（PCR）方法以特异性和定量的方式检测链格孢（Alternaria brassicicola），因此引物和探针可用于客观定量诊断和 进展黑点。
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公开(公告)号：KR1020130005986A
公开(公告)日：2013-01-16
申请号：KR1020110067744
申请日：2011-07-08
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A rice blast resistant OsABP57 genes is provided to develop rice blast resistant breed, and to improve crop productivity. CONSTITUTION: A rice blast resistant protein has amino acid of sequence number 2. A gene encoding the protein has a base of sequence number 1. A transformant with improved rice blast resistance is prepared by transforming with a recombinant expression vector containing the gene of sequence number 1. The rice blast resistance is controlled by regulating protein level in a cell. A method for over-expressing the protein comprises: a step of preparing the recombinant expression vector and transforming a plant with the recombinant expression vector or a step of transducing the gene encoding the protein into plant cells. The plant is Oryza sativa, barley, wheat, rye, oat, grass, hog millet, corn, onion, leek, garlic, Anemarrhenae Rhizoma, Polygonatum falcatum, Veratri Rhizoma, bamboo, Cymbidium goeringii, Cymbidium ssp., Dendrobium phalaenopsis, Neofinetia falcate, Aerides japonicum, Cattleya ssp., Oncidium ssp., Brassia ssp., or Cymbidium Kanran.
Abstract translation: 目的：提供抗稻瘟病OsABP57基因，开发抗稻瘟病品种，提高作物生产力。 构成：抗稻瘟病蛋白具有序列号2的氨基酸。编码蛋白质的基因具有序列号1的碱基。通过用含有序列号基因的重组表达载体转化来制备具有改善的稻瘟病抗性的转化体 通过调节细胞中的蛋白质水平来控制稻瘟病抗性。 用于过表达蛋白质的方法包括：制备重组表达载体并用重组表达载体转化植物的步骤或将编码蛋白质的基因转导到植物细胞中的步骤。 该植物是水稻，大麦，小麦，黑麦，燕麦，禾谷，粟，玉米，洋葱，韭菜，大蒜，Anemarrhenae Rhizoma，茯苓，藜，竹， ，日本Aer，属，Cattleya ssp。，Oncidium ssp。，Brassia ssp。，或Cymbidium Kanran。
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公开(公告)号：KR1020120128878A
公开(公告)日：2012-11-28
申请号：KR1020110046796
申请日：2011-05-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8205 , A01H4/008 , C12N5/0025 , C12N15/8209 , C12N15/8216
Abstract: PURPOSE: A method for transforming a plant using Agrobacteria is provided to remarkably improve transformation efficiency. CONSTITUTION: A method for trasforming a plant body using Agrobacteria comprises a step of removing albumen from the plant body and co-culturing the plant body with the Agrobacteria containing a recombinant expression vector for 7 or more days. The plant is cultured is cultured in an N6 meidum containing 1-3 mg/L of 2,4-dichlorophenoxyacetic acid at 25-32 Deg. C. for 4-7 days before removing albumen. The plant body is a callus of Oryza sativa. The Agrobacteria is Agrobacterium thumefacience strain LBA4404.
Abstract translation: 目的：提供使用农杆菌转化植物的方法，以显着提高转化效率。 构成：使用农杆菌转化植物体的方法包括从植物体中除去白蛋白并与含有重组表达载体的农杆菌共培养植物体7天或更多天的步骤。 将培养的植物在含有1-3mg / L 2,4-二氯苯氧基乙酸的N6薄荷中以25-32度进行培养。 在清除白蛋白前4-7天。 植物体是水稻的愈伤组织。 农杆菌是土壤杆菌耐药菌株LBA4404。
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公开(公告)号：KR1020120064521A
公开(公告)日：2012-06-19
申请号：KR1020100125797
申请日：2010-12-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8225 , C07K14/415 , C12N15/8205 , C12N15/8251 , C12N15/8273
Abstract: PURPOSE: A promoter which is expressed in guard cells and a recombinant expression vector containing the same are provided to enhance various environmental stress resistance. CONSTITUTION: A promoter for target protein-specific expression in plant leaves has a base of sequence number 1. The part of the plant leaves is guard cells. A recombinant expression vector contains a promoter for target protein-specific expression and a structural gene encoding the target protein. A transformant prepared by the expression vector is E.coli, Bacillus subtilis, Streptomyces, Pseudomonas, Proteus mirabilis, Staphylococcus, or Agrobacterium tumefaciens.
Abstract translation: 目的：提供在保卫细胞中表达的启动子和含有其的重组表达载体以增强各种耐环境胁迫性。 构成：植物叶中靶蛋白特异性表达的启动子具有序列号1的基础。植物叶的部分是保卫细胞。 重组表达载体含有靶蛋白特异性表达的启动子和编码靶蛋白的结构基因。 由表达载体制备的转化体是大肠杆菌，枯草芽孢杆菌，链霉菌，假单胞菌，奇异变形杆菌，葡萄球菌或根癌土壤杆菌。
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