公开(公告)号：KR1020040051385A

公开(公告)日：2004-06-18

申请号：KR1020020079311

申请日：2002-12-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 홍연규 ,  정명근 ,  송석보 ,  이봉춘 ,  이동창 ,  김순철

IPC: A01N63/02

Abstract: PURPOSE: Provided is an isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens), which is released from culture extract of isolate BWC98-105(Sclerotinia sp.), thereby withering the subterranean stem and stem of the white clover continuously, and inhibiting the generation thereof consequently to reduce the consumption of agricultural chemicals. CONSTITUTION: An isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens) by using BWC98-105(Sclerotinia sp.) comprises the steps of: culturing BWC98-105 strain in PD broth medium for 7 days, followed by systematic extraction with an organic solvent; fractioning the extraction with butanol and ethylacetate; separating the butanol and ethylacetate fractions using the column filled with reverse phase silica gel(C18); and analyzing the separated fractions by HPLC, wherein a second metabolic product having herbicidal effect on the white clover(Trifolium repens) is produced at retention time intervals of 4.13 minute.

Abstract translation: 目的：提供从分离株BWC98-105（核盘菌属）的培养提取物中释放的白三叶草（Trifolium repens）上具有除草效果的次生代谢产物的分离方法，从而使地下茎和茎枯萎 白三叶草不断，抑制其产生，从而减少农药的消耗。 构成：使用BWC98-105（核盘菌属）对白三叶草（Trifolium repens）具有除草效果的次生代谢产物的分离方法包括以下步骤：在PD肉汤培养基中培养BWC98-105菌株7天，随后 通过有机溶剂的系统萃取; 用丁醇和乙酸乙酯分级萃取; 使用填充有反相硅胶（C18）的柱分离丁醇和乙酸乙酯级分; 并通过HPLC分析分离的级分，其中在4.13分钟的保留时间间隔产生对白三叶草（Trifolium repens）具有除草效果的第二代谢产物。

公开(公告)号：KR20180028632A

公开(公告)日：2018-03-19

申请号：KR20160116175

申请日：2016-09-09

Applicant: 강원대학교산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 정명근 ,  황영선 ,  김선태 ,  윤원병 ,  한원영 ,  강항원

IPC: G01N33/00

CPC classification number: G01N33/0098 ,  G01N2333/415

Abstract: 본발명은대사체기술을이용한콩의퇴화종실판별방법에관한것으로, 본발명의식물의퇴화종실을판별하는방법을통해, 식물의퇴화종실을발아전에판별하고제거하여경제적인손실을예방할수 있으며, 발아율이매우높은고품질의종실만을선별함으로써종실품질향상에의한종실의부가가치를높일수 있다.

Abstract translation: 本发明能够防止经济损失通过该方法涉及一种方法，变性种子使用的代谢物的技术，以确定本发明的植物的变性种子确定大豆，确定发芽前植物的变性种子和去除，并且 通过仅选择具有非常高的发芽率的高质量苗，可以通过改善苗的质量来增加苗的附加值。

公开(公告)号：KR1020110088304A

公开(公告)日：2011-08-03

申请号：KR1020100008156

申请日：2010-01-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 한상익 ,  하태정 ,  장기창 ,  서우덕 ,  이제봉 ,  고종민 ,  오성환 ,  김상열 ,  박동수 ,  조준현 ,  강항원 ,  정명근

IPC: G01N33/10 ,  G01N33/02 ,  G01N31/00 ,  G01N21/359

Abstract: PURPOSE: A non-destructive analysis method is provided to effectively analyze ingredient consisting of fatty acid of a grain. CONSTITUTION: A non-destructive analysis method for a fatty acid composition of a single granule comprises: a step of randomly selecting a plurality of prediction model development group and prediction model evaluation group; a step of measuring near infrared absorption spectrum of two groups; a step of randomly selecting grain granule sample among the prediction model development groups and measuring near infrared ray absorption spectrum; a step of pulverizing the granule sample and performing chemical analysis of fatty acid ingredients; and a step of applying primary and secondary derivatives to the absorption spectrum.

Abstract translation: 目的：提供一种非破坏性分析方法，有效分析由谷物脂肪酸组成的成分。 构成：单粒子脂肪酸组成的无损分析方法包括：随机选择多个预测模型开发组和预测模型评价组的步骤; 测量两组近红外吸收光谱的步骤; 在预测模型开发组中随机选择颗粒样品并测量近红外线吸收光谱的步骤; 粉碎颗粒样品并进行脂肪酸成分的化学分析的步骤; 以及将一次和二次衍生物应用于吸收光谱的步骤。

公开(公告)号：KR101885652B1

公开(公告)日：2018-08-06

申请号：KR1020160116175

申请日：2016-09-09

Applicant: 강원대학교산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 정명근 ,  황영선 ,  김선태 ,  윤원병 ,  한원영 ,  강항원

IPC: G01N33/00

Abstract: 본발명은대사체기술을이용한콩의퇴화종실판별방법에관한것으로, 본발명의식물의퇴화종실을판별하는방법을통해, 식물의퇴화종실을발아전에판별하고제거하여경제적인손실을예방할수 있으며, 발아율이매우높은고품질의종실만을선별함으로써종실품질향상에의한종실의부가가치를높일수 있다.

公开(公告)号：KR1020030010025A

公开(公告)日：2003-02-05

申请号：KR1020010044760

申请日：2001-07-25

Applicant: 대한민국(농촌진흥청장)

Inventor： 정명근 ,  백인열 ,  강성택 ,  한원영 ,  신두철 ,  곽용호 ,  문헌팔

IPC: G01N21/359

Abstract: PURPOSE: A method for non-destructive analysis of one seed grain by near infrared ray absorption spectrum is provided to precisely analyze the components and characteristics of one seed grain in a short time without using any additional chemical analysis equipments. CONSTITUTION: A method for non-destructive analysis of one seed grain by near infrared ray absorption spectrum includes the steps of measuring near infrared ray absorption spectrums(400-2500mm) of 30-100 seeds of selected grain samples by a horizontal manual module, correcting absorbancy per wavelength measured by 30-100 grains respectively with a single average absorption spectrum per wavelength, randomly distinguishing 150 averaged absorption spectrum into about 100 estimation model developing models and about 50 estimation model testing models, correcting the uniformity of the samples by using a sieve of 60 mesh after preparing powdered samples, testing the moisture content of the powdered samples by drying for 2 hours in a drier of 105°C, precisely carrying out chemical analysis of contents of components such as protein and fat contained in the powdered samples twice or more, removing samples of which a difference in the chemical analysis is beyond a range of 0.2-0.4% from the models, inserting real component contents of which moisture contents are corrected into the measured average absorption spectrum, carrying out the statistic analysis for setting optimum estimation models for the respective components, and selecting the optimum estimation models by discriminating the precision of the estimation models.

Abstract translation: 目的：提供一种通过近红外线吸收光谱对一颗种子进行无损分析的方法，在不使用任何其他化学分析设备的情况下，在短时间内精确分析一种种籽的成分和特征。 构成：通过近红外线吸收光谱对一种种子颗粒进行非破坏性分析的方法包括以下步骤：通过水平手动模块测量所选粒度样品的30-100粒种子的近红外线吸收光谱（400-2500mm），校正 每个波长的吸光度分别用30-100粒度测量，每个波长具有单个平均吸收光谱，将150个平均吸收光谱随机区分为约100个估计模型开发模型和约50个估计模型测试模型，通过使用筛子校正样品的均匀性 在制备粉末样品后为60目，通过在105℃的干燥器中干燥2小时来测试粉末样品的水分含量，精确地对粉末样品中包含的蛋白质和脂肪等组分的内容物进行化学分析两次或 更多的是，去除化学分析差异的样品超出了mod的0.2-0.4％的范围 将水分含量被校正的实部内容插入到测量的平均吸收光谱中，进行统计分析以设定各组分的最佳估计模型，并通过区分估计模型的精度来选择最佳估计模型。

公开(公告)号：KR100514430B1

公开(公告)日：2005-09-14

申请号：KR1020020079311

申请日：2002-12-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 홍연규 ,  정명근 ,  송석보 ,  이봉춘 ,  이동창 ,  김순철

IPC: A01N63/02

Abstract: 본 발명은 클로버에 대하여 기주특이적으로 기생하고 제초활성을 갖는 스클레로티니아 트리폴리오럼 BWC98-105 균주를 이용하여 이 균주가 분비하는 클로버 제초활성 2차 대사산물(secondary product)을 분리하는 방법에 관한 것으로, 스클레로티니아 트리폴리오럼 BWC98-105 균주(KACC93004)를 PD Broth 배지에서 7일 동안 액체 배양하는 단계; 상기 배양액을 여과하는 단계; 상기 여과된 배양액을 헥산, 클로로포름, 에틸아세테이트 및 부탄올 순으로 액체분배추출하고, 에틸아세테이트 및 부탄올 각각의 분획물을 혼합하는 단계; 상기 분획 혼합물을 역상 실리카겔(C
18 )로 충진된 컬럼으로 분리하는 단계; 및 상기에서 분리된 분리물을 고속액체색층분석기(HPLC)를 이용하여 4.13분의 머무름 시간에 추출되는 화합물을 수득하는 단계;를 포함하여 이루어진다.
본 발명의 분리방법으로 얻어지는 대사물질은 신속히 클로버 지하경 및 줄기 등 지상부 식물체를 고사시키고, 다음해의 잔디 포장을 오염시키는 클로버의 발생을 억제시키는 효과가 있다.

公开(公告)号：KR101947238B1

公开(公告)日：2019-02-12

申请号：KR1020160145507

申请日：2016-11-03

Applicant: 강원대학교산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 윤원병 ,  박현우 ,  한원영 ,  강항원 ,  정명근 ,  김선태

IPC: G01N33/02 ,  G06T7/00

公开(公告)号：KR100433263B1

公开(公告)日：2004-05-31

申请号：KR1020010044760

申请日：2001-07-25

Applicant: 대한민국(농촌진흥청장)

Inventor： 정명근 ,  백인열 ,  강성택 ,  한원영 ,  신두철 ,  곽용호 ,  문헌팔

IPC: G01N21/359

Abstract: PURPOSE: A method for non-destructive analysis of one seed grain by near infrared ray absorption spectrum is provided to precisely analyze the components and characteristics of one seed grain in a short time without using any additional chemical analysis equipments. CONSTITUTION: A method for non-destructive analysis of one seed grain by near infrared ray absorption spectrum includes the steps of measuring near infrared ray absorption spectrums(400-2500mm) of 30-100 seeds of selected grain samples by a horizontal manual module, correcting absorbancy per wavelength measured by 30-100 grains respectively with a single average absorption spectrum per wavelength, randomly distinguishing 150 averaged absorption spectrum into about 100 estimation model developing models and about 50 estimation model testing models, correcting the uniformity of the samples by using a sieve of 60 mesh after preparing powdered samples, testing the moisture content of the powdered samples by drying for 2 hours in a drier of 105°C, precisely carrying out chemical analysis of contents of components such as protein and fat contained in the powdered samples twice or more, removing samples of which a difference in the chemical analysis is beyond a range of 0.2-0.4% from the models, inserting real component contents of which moisture contents are corrected into the measured average absorption spectrum, carrying out the statistic analysis for setting optimum estimation models for the respective components, and selecting the optimum estimation models by discriminating the precision of the estimation models.

Abstract translation: 目的：提供一种通过近红外线吸收光谱对一粒种子进行无损分析的方法，可在短时间内精确分析一粒种子的成分和特性，而无需使用任何额外的化学分析设备。 本发明涉及一种用近红外线吸收光谱对一粒种子进行无损分析的方法，它包括以下步骤：通过水平手动模块测量30-100粒种子的选定颗粒样品的近红外线吸收光谱（400-2500mm），校正 每个波长的吸光度分别用每个波长的单个平均吸收光谱测量30-100个颗粒，随机区分150个平均吸收光谱为约100个估计模型开发模型和约50个估计模型测试模型，使用筛子校正样品的均匀性 制备粉末样品后，通过在105℃干燥器中干燥2小时测试粉末样品的含水量，精确地对粉末样品中含有的蛋白质和脂肪等组分的含量进行化学分析 两次或更多次，从化学分析的差异超过0.2-0.4％的范围内移除样品 将其中含水量被修正的实际成分含量插入到测量的平均吸收光谱中，进行统计分析以设置各个成分的最佳估计模型，并通过区分估计模型的精度来选择最佳估计模型。