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公开(公告)号：KR1020140058737A
公开(公告)日：2014-05-15
申请号：KR1020120124210
申请日：2012-11-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/825 , C12N9/0073 , C12N9/90 , C12N15/8205 , C12N15/8257 , C12Y114/13129 , C12Y503/99008
Abstract: The present invention relates to transgenic rice producing capsanthin, which is obtained by breeding rice transformed with a plant transformation expression vector containing a capsanthin-capsorubin synthase gene and zeaxanthin-producing rice transformed with a plant transformation expression vector containing a β-carotene hydroxylase gene. The transgenic rice according to the present invention has a high content of zeaxanthin and exhibits red color while producing capsanthin, a red pigment of red pepper, and thus can be used as functional special crops.
Abstract translation: 本发明涉及转基因水稻生产辣椒红的方法，所述转基因水稻生产辣椒红是通过用含有辣椒红 - 油蛋白合成酶基因的转基因植物转化表达载体和用含有β-胡萝卜素羟化酶基因的植物转化表达载体转化的玉米黄质生产水稻而转化的水稻获得的。 根据本发明的转基因水稻具有高含量的玉米黄质并显示红色，同时产生辣椒红，红辣椒红色素，因此可用作功能性特殊作物。
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公开(公告)号：KR101303802B1
公开(公告)日：2013-09-09
申请号：KR1020110097012
申请日：2011-09-26
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 지아산틴 생성능력이 우수한 재조합 벡터 및 상기 재조합 벡터로 형질전환된 형질전환 세포 및 식물에 관한 것이다.
본 발명에 따른 재조합 벡터 및 형질전환 세포를 이용하면 카로티노이드 물질 중 특히 지아산틴 함량이 높은 형질전환 식물을 제조할 수 있는 바, 시력개선 효과 등을 기대할 수 있는 기능성 특수 작물을 제공할 수 있다. 본 발명에 따른 형질전환 식물은 지아산틴 함량이 증가되어 황금색을 나타내므로, 이를 이용하여 항산화성 천연색소 생산을 통한 새로운 컬러작물 개발 기반을 확립하고, 새로운 기능성 컬러 GM 작물 소재 개발을 위한 고유 유전자 및 운반체 IP를 확보할 수 있다. 또한 자연계에 없는 새로운 특수 작물 개발로 장기적으로 기능성 작물의 다양성 확대 및 소비 촉진 기대되며 지아산틴 생산작물의 사료화 등을 통한 농업의 신규한 산업 소재 개발에 도움이 될 수 있다.-
公开(公告)号：KR101229885B1
公开(公告)日：2013-02-08
申请号：KR1020110097049
申请日：2011-09-26
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/825 , A61K8/97 , A61Q19/02 , C12N15/8205 , C12N15/8257
Abstract: PURPOSE: A novel polynucleotide which generates keto-carotenoid such as astaxanthin is provided to produce a transgenic plant which generates two kinds of keto-carotenoid(astaxanthin and canthaxanthin). CONSTITUTION: A novel polynucleotide which generates keto-carotenoid such as astaxanthin contains a nucleic acid of sequence number 1 or 2. A recombinant vector containing the polynucleotide of sequence number 1 or 2 additionally contains a polynucleotide with a nucleic acid of sequence number 3 or 4. The recombinant vector is pGlb:BAC, pGlb:stBAC, pGlb:BAC-PAC, or pGlb:stBAC-PAC vector. A cell which is transformed with the recombinant vector is an Agrobacterium sp. microorganism or a plant cell. A transgenic plant containing the recombinant vector is Oryza sativa, corn, or barley. [Reference numerals] (AA) Normal rice; (BB) Beta-carotene rice; (CC) Astaxanthin rice; (DD) Recombinant gene name; (EE) Patent no. 10-0905219; (FF) Reference
Abstract translation: 目的：提供产生酮类胡萝卜素（如虾青素）的新型多核苷酸以产生产生两种类型的类胡萝卜素（虾青素和角黄素）的转基因植物。 构成：产生酮类胡萝卜素如虾青素的新型多核苷酸含有序列号1或2的核酸。含有序列号1或2的多核苷酸的重组载体另外含有具有序列号3或4的核酸的多核苷酸 重组载体是pGlb：BAC，pGlb：stBAC，pGlb：BAC-PAC或pGlb：stBAC-PAC载体。 用重组载体转化的细胞是农杆菌属。 微生物或植物细胞。 含有重组载体的转基因植物是水稻，玉米或大麦。 （附图标记）（AA）正常米; （BB）β-胡萝卜素水稻; （CC）虾青素米; （DD）重组基因名称; （EE）专利号 10-0905219; （FF）参考
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公开(公告)号：KR1020110127982A
公开(公告)日：2011-11-28
申请号：KR1020100047543
申请日：2010-05-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/823 , C07K14/415 , C12N15/8205 , C12N15/8206
Abstract: PURPOSE: A promoter which petal-specifically induces expression of a foreign gene in a plant is provided to be used for transformation of various crops in a genetic engineering method. CONSTITUTION: A petal-specific promoter is a promoter of Nicotiana tabacum ANS(anthocyanidin synthase) gene of sequence number 1 or 2. The promoter induces the expression of petal-specific gene. The promoter has a base sequence of sequence number 3. A primer set for amplifying the promoter contains a primer of sequence number 4 and a primer of sequence number 5. A pBGWFS7-PNtANS expression vector contains the promoter. A method for petal-specifically expressing a foreign gene comprises: a step of preparing an expression vector containing the promoter and the foreign gene; and a step of transducing the expression vector to a plant.
Abstract translation: 目的：提供花瓣特异性诱导外源基因在植物中表达的启动子，用于基因工程方法转化各种作物。 构成：花瓣特异性启动子是序列号1或2的烟草烟草（Citotiana tabacum）ANS（花青素合酶）基因的启动子。启动子诱导花瓣特异性基因的表达。 启动子具有序列号3的碱基序列。用于扩增启动子的引物组含有序列号4的引物和序列号5的引物.pBGWFS7-PNtANS表达载体含有启动子。 花瓣特异性表达外源基因的方法包括：制备含有启动子和外源基因的表达载体的步骤; 以及将表达载体转导到植物的步骤。
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公开(公告)号：KR1020100049335A
公开(公告)日：2010-05-12
申请号：KR1020080108451
申请日：2008-11-03
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8216 , C12N9/20
Abstract: PURPOSE: A novel plant constitutive promoter is provided to express a foreign gene regardless of expression place and to develop transgenic plant. CONSTITUTION: A plant constitutive promoter contains partial or whole base of sequence number 1. The constitutive promoter is derived from Arabidopsis thaliana. A plant constitutive promoter, Lip2 is obtained by performing PCR of oligonucleotide of sequence numbers 6 and 7. A recombinant expression vector pBGWFS7-PAtLip3(deposit number: KACC 95086P) contains the plant constitutive promoter. The plant expression vector which is able to express a target gene is prepared using the constitutive promoter.
Abstract translation: 目的：提供一种新颖的植物组成型启动子来表达外源基因，不论其表达位置如何，并开发转基因植物。 构成：植物组成型启动子含有序列号1的部分或全部碱基。组成型启动子来源于拟南芥。 通过进行序列号6和7的寡核苷酸的PCR获得植物组成型启动子Lip2。重组表达载体pBGWFS7-PAtLip3（保藏号：KACC 95086P）含有植物组成型启动子。 使用组成型启动子制备能够表达靶基因的植物表达载体。
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Patent Agency Ranking
公开(公告)号：KR100953763B1
公开(公告)日：2010-04-21
申请号：KR1020080023031
申请日：2008-03-12
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물체 내에 외래 유용유전자의 발현 장소를 뿌리 부분으로 제한하여 발현시키는 뿌리 특이 발현 프로모터에 관한 것으로서, 더욱 상세하게는 서열번호:1로 구성되는 뿌리 특이 발현 프로모터, 서열번호:2로 구성되는 올리고 누클레오티드 및 서열번호:3으로 구성되는 올리고누클레오티드로 이루어지는 상기 뿌리 특이 프로모터를 증폭하기 위한 프라이머, 상기 서열번호:1의 프로모터 서열을 포함하는 것을 특징으로 하는 발현벡터 pBGWFS7-PAtM19PK 및 상기 발현벡터 pBGWFS7-PAtM19PK으로 형질전환된 식물세포에 관한 것이다.
뿌리특이, 프로모터, 애기장대, 조직특이, 형질전환체-
7.发明授权
公开(公告)号：KR100905219B1
公开(公告)日：2009-07-01
申请号：KR1020070087608
申请日：2007-08-30
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 자가 절단 2A배열을 포함하는 베타카로틴 생합성용 융합 폴리뉴클레오티드 및 이를 이용한 형질전환 식물체에 관한 것으로서 보다 상세하게는 파이토엔 합성 효소, FMDV 유래 2A 서열 및 박테리아 카로틴 불포화 효소를 암호화하는 융합 폴리뉴클레오티드 및 이를 이용한 형질전환 식물체에 관한 것이다. 본 발명의 융합 폴리뉴클레오티드 및 이를 이용한 재조합 벡터는 형질전환된 세포 내에서 안정적으로 파이토엔 합성효소 및 카로틴 불포화 효소를 발현할 수 있는 효과를 갖는다. 따라서 본 발명의 융합 폴리뉴클레오티드는 베타카로틴 생합성을 위하여 식물체의 대사과정의 조절의 목적으로 사용할 수 있을 뿐만 아니라, 이로 인해 유용한 대사산물인 베타카로틴 함량의 증진의 목적으로 사용할 수 있다.
유전자 다중 발현, 2A 배열, 파이토엔 합성 효소, 카로틴 불포화 효소, 베타카로틴, 벼, 쌀-
8.发明公开
公开(公告)号：KR1020090022328A
公开(公告)日：2009-03-04
申请号：KR1020070087608
申请日：2007-08-30
Applicant: 대한민국(농촌진흥청장)
Abstract: A fusion polynucleotide with self-cleaving 2A arrangement is provided to control a metabolism process of a plant for beta-carotene biosynthesis, thereby increasing the contents of beta-carotene. A fusion polynucleotide with self-cleaving 2A arrangement encodes hot pepper phytoene synthase consisting of base sequences indicated as a sequence number 1, rice plant opimization FMDV-derived 2A sequence, and bacteria carotene desaturase. A recombinant vector for transformation comprises a promoter and the fusion polynucleotide operably connected to the promoter. The recombinant vector is a pGlb-PAC vector.
Abstract translation: 提供具有自我裂解2A排列的融合多核苷酸以控制植物的β-胡萝卜素生物合成的代谢过程，从而增加β-胡萝卜素的含量。 具有自切割2A排列的融合多核苷酸编码由序列号1表示的碱基序列，水稻植物优化的FMDV衍生的2A序列和细菌胡萝卜素去饱和酶组成的辣椒八味子合成酶。 用于转化的重组载体包含与启动子可操作地连接的启动子和融合多核苷酸。 重组载体是pGlb-PAC载体。
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9.发明公开
公开(公告)号：KR1020080075421A
公开(公告)日：2008-08-18
申请号：KR1020070014589
申请日：2007-02-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A fusion protein of Escherichia coli enterotoxin protein and a neuraminidase epitope of Newcastle disease virus is provided to increase immunity against Newcastle disease virus in poultry, so that the protein is useful for prevention of Newcastle disease virus. A fusion protein of E. coli heat-labile enterotoxin subunit B and a neuraminidase epitope of Newcastle disease virus has the amino acid sequence of SEQ ID NO:1. A polynucleotide encoding the fusion protein has the nucleotide sequence of SEQ ID NO:2. An expression vector pLD-CtV-HN contains the polynucleotide of SEQ ID NO:2. A vaccine composition for preventing Newcastle disease virus contains the fusion protein as an effective ingredient. The immunity against Newcastle disease virus in poultry is increased by administering the fusion protein or a transgenic plant containing the expression vector pLD-CtV-HN into poultry.
Abstract translation: 提供大肠杆菌肠毒素蛋白的融合蛋白和新城疫病毒的神经氨酸酶表位，以增加对家禽新城疫病毒的免疫力，使得该蛋白质可用于预防新城疫病毒。 大肠杆菌热不稳定肠毒素亚基B和新城疫病毒的神经氨酸酶表位的融合蛋白具有SEQ ID NO：1的氨基酸序列。 编码融合蛋白的多核苷酸具有SEQ ID NO：2的核苷酸序列。 表达载体pLD-CtV-HN含有SEQ ID NO：2的多核苷酸。 用于预防新城疫病毒的疫苗组合物含有融合蛋白作为有效成分。 通过将融合蛋白或含有表达载体pLD-CtV-HN的转基因植物施用于家禽来增加家禽中新城疫病毒的免疫力。
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公开(公告)号：KR1020130055708A
公开(公告)日：2013-05-29
申请号：KR1020110097012
申请日：2011-09-26
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/825 , C12N9/001 , C12N9/1085 , C12N15/8205 , C12N15/8257 , C12Y103/99026 , C12Y205/01032
Abstract: PURPOSE: A recombinant vector and a transformed cell are provided to obtain a transgenic plant with high zeaxanthin content, to improve visual acuity, and to obtain a gene and carrier IP for developing a novel functional color GM(genetic modified) crop material. CONSTITUTION: A polynucleotide is denoted by sequence number 3 or 4. A recombinant vector containing the polynucleotide is pGlb:Bch or pGlb:stBch. A transformed cell is prepared by transforming with the vector and includes Agrobacterium sp. microorganism or a plant cell. A transgenic plant is prepared by introducing the vector. A transgenic rice plant contains the vector. [Reference numerals] (AA) Normal rice; (BB) Beta carotene rice; (CC) Zeaxanthin rice; (DD) Recombination gene name; (EE) Patent No. 10-0905219; (FF) Reference
Abstract translation: 目的：提供重组载体和转化细胞以获得具有高玉米黄质含量的转基因植物，以改善视力，并获得用于开发新型功能性颜色GM（遗传修饰）作物材料的基因和载体IP。 构成：多核苷酸由序列号3或4表示。含有多核苷酸的重组载体是pGlb：Bch或pGlb：stBch。 通过用载体转化制备转化细胞，并且包括农杆菌属 微生物或植物细胞。 通过引入载体制备转基因植物。 转基因水稻植物含有载体。 （附图标记）（AA）正常米; （BB）β-胡萝卜素米; （CC）玉米黄质米; （DD）重组基因名称; （EE）专利号10-0905219; （FF）参考
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