公开(公告)号：KR1020040031277A

公开(公告)日：2004-04-13

申请号：KR1020020060660

申请日：2002-10-04

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  최정환

IPC: C12N15/63

CPC classification number: C12N15/85 ,  C12Q1/34 ,  G01N33/5058 ,  G01N33/6812 ,  G01N2333/938

Abstract: PURPOSE: An aplysia two-hybrid system is provided, thereby verifying correlation between transcription factors during memory process, so that it can be useful for the examination of mind disease causing factors and screening of drugs relating to correlation between proteins. CONSTITUTION: An aplysia two-hybrid system comprises (a) a reporter vector 4xGal4-LacZ containing Gal4 binding domain and LacZ gene; (b) pNEX-Gal4DB-bait vector containing Gal4 DNA binding domain-bait polynucleotide in pNEX vector; and (c) pNEXδ-Gal4AD prey vector containing Gal4 transcription activation domain in pNEXδ vector, wherein the bait and prey polynucleotides encode transcription factors during memory process; the bait and prey polynucleotide is selected from ApCREB1a, ApCREB2 and ApC/EBP. A method for examining correlation between proteins comprises micro-injecting the aplysia two-hybrid system into an aplysia nerve cell, and analyzing beta-galactosidase from the aplysia nerve cell through X-gal dyeing.

Abstract translation: 目的：提供aplysia双杂交系统，从而验证记忆过程中转录因子之间的相关性，从而可用于检查心脑疾病引起因素和筛选与蛋白质之间的相关性相关的药物。 构成：aplysia双杂交体系包含（a）含有Gal4结合结构域和LacZ基因的报道载体4xGal4-LacZ; （b）pNEX载体中含有Gal4 DNA结合域诱饵多核苷酸的pNEX-Gal4DB-诱饵载体; 和（c）pNEXδ载体中含有Gal4转录激活结构域的pNEXδ-Gal4AD猎物载体，其中诱饵和猎物多核苷酸在记忆过程中编码转录因子; 诱饵和猎物多核苷酸选自ApCREB1a，ApCREB2和ApC / EBP。 一种检测蛋白质相关性的方法包括将aplysia双杂交系统微注射到神经细胞，并通过X-gal染色分析来自神经细胞膜神经细胞的β-半乳糖苷酶。

公开(公告)号：KR100813610B1

公开(公告)日：2008-03-13

申请号：KR1020060038615

申请日：2006-04-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  장동혁

IPC: C07K14/435 ,  C12N15/12

Abstract: 본 발명은 참군소 유래의 신규한 코필린 및 이를 이용하여 신경 퇴행성 질환에 유용한 활성물질의 스크리닝 시스템에 관한 것으로, 신경 세포 내 액틴과 결합하는 활성을 갖는 참군소 유래의 코필린을 이용하여 신경 퇴행성 질환의 예방 및 치료에 유용한 활성물질을 스크리닝 방법을 제공함으로써, 미지의 약물 후보군에서 신경 퇴행성 질환을 예방 및 치료할 수 있는 물질을 용이하게 탐색할 수 있게 된 것이다.
신경 퇴행성 질환, 참군소, 코필린, 항체, 스크리닝

公开(公告)号：KR1020040019613A

公开(公告)日：2004-03-06

申请号：KR1020020051211

申请日：2002-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  장덕진

IPC: C12N15/12

CPC classification number: C07K14/43504 ,  C12N5/0619 ,  C12N15/85 ,  C12N15/89

Abstract: PURPOSE: An octopamine receptor isolated from Aplysia kurodai and an activation method of PKA(cAMP-dependent protein kinase) pathway are provided, thereby improving short-period memory and screening a drug activating the PKA pathway to improve memory. CONSTITUTION: An octopamine receptor gene(Ap oa1) isolated from Aplysia kurodai encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 21, wherein the octopamine receptor gene(Ap oa1) has the nucleotide sequence set forth in SEQ ID NO: 20. A neuron expression system comprises (a) a vector containing a membrane receptor gene; and (b) a vector-microinjected neuron, wherein the membrane receptor gene encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 21; and the membrane receptor gene has the nucleotide sequence set forth in SEQ ID NO: 20; the vector is pNEXδ-Ap oa1 or pNEX3-Ap oa1-HA. An activation method of PKA pathway comprises (a) transduction of a vector containing the octopamine receptor gene into the neuron; (b) selecting a neuron expressing the octopamine receptor; and (c) treating the selected neuron with octopamine.

Abstract translation: 目的：提供从黑腹苷酸分离的章鱼胺受体和PKA（cAMP依赖性蛋白激酶）途径的活化方法，从而改善短期记忆和筛选激活PKA途径的药物以改善记忆。 构成：从黑腹果蝇分离的章鱼胺受体基因（Apo1）编码具有SEQ ID NO：21所示的氨基酸序列的多肽，其中所述章鱼胺受体基因（Apoa1）具有SEQ ID NO 20.神经元表达系统包含（a）含有受体基因的载体; 和（b）载体微注射神经元，其中所述膜受体基因编码具有SEQ ID NO：21所示氨基酸序列的多肽; 膜受体基因具有SEQ ID NO：20所示的核苷酸序列; 载体是pNEXδ-Apoa1或pNEX3-Apoa1-HA。 PKA途径的活化方法包括（a）将含有章鱼胺受体基因的载体转导入神经元; （b）选择表达章鱼胺受体的神经元; 和（c）用章鱼胺处理所选择的神经元。

公开(公告)号：KR100779940B1

公开(公告)日：2007-11-29

申请号：KR1020060053465

申请日：2006-06-14

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  이승희

IPC: C07K14/00 ,  A61K36/8888

CPC classification number: C07K14/47 ,  A61K38/1709

Abstract: A functional protein is provided to activate or inhibit the function in a specific brain area and convert the short term memory into the long term memory, thereby being applicable as a core element of forming a controllable artificial intelligence model. A functional protein includes an amino acid sequence described as SEQ ID : NO. 2. A gene encoding the protein is a sequence described as SEQ ID : NO. 1. A pharmaceutical composition for improving memory comprises the protein as an effective ingredient.

Abstract translation: 提供功能性蛋白质以激活或抑制特定脑区域中的功能并将短期记忆转换成长期记忆体，从而可用作形成可控人造智能模型的核心要素。 功能蛋白包括SEQ ID NO：1所示的氨基酸序列。 编码蛋白质的基因是SEQ ID NO： 1.一种用于改善记忆的药物组合物，其包含该蛋白质作为有效成分。

公开(公告)号：KR100552402B1

公开(公告)日：2006-02-20

申请号：KR1020030019712

申请日：2003-03-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  허민정

IPC: C07K14/47

Abstract: 본 발명은 시냅스 가소성(synaptic plasticity) 및 유전자 전달(gene transfer) 연구에 유용한 새로운 기능을 가지는 단백질에 관한 것으로 상기 단백질은 GEF(GDP/GTP exchange 팩터) 활성 의존적인 방법으로 신경돌기 생성과 시냅스 전달을 조절하며, 세포골격 조절을 통하여 신경돌기 생성을 유발하고, GEF 활성 의존적인 방법으로 정맥류(varicosity)를 증가시킨다.
msec7-1, GDP/GTP Exchange 팩터, ADP 리보실레이션 팩터, 신경돌기 생성, 감각-운동 공배양, 시냅스 전달

公开(公告)号：KR100471605B1

公开(公告)日：2005-03-10

申请号：KR1020020060660

申请日：2002-10-04

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  최정환

IPC: C12N15/63

Abstract: 본 발명은 군소 투-하이브리드 시스템에 관한 것이다. 특히 본 발명은 기억과정에서 전사인자들간의 상호작용을 확인할 수 있으며, 단백질간의 상호작용에 관여하는 약물을 스크리닝하는 용도로 활용할 수 있는 4xGal4-LacZ 벡터, pNEX-Gal4DB-베이트(bait) 벡터 및 pNEXδGal4AD-프레이(prey) 벡터를 포함하는 군소 투-하이브리드 시스템을 제공한다.

公开(公告)号：KR1020040073666A

公开(公告)日：2004-08-21

申请号：KR1020030009329

申请日：2003-02-14

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  이진아

IPC: C07K14/47

CPC classification number: C07K14/43504

Abstract: PURPOSE: An inhibitor of long-term synaptic facilitation is provided, thereby effectively inhibiting the long-term synaptic facilitation by controlling the expression of ApCREB2(cyclic AMP-response element binding protein 2), so that it can be useful for study on the long-term synaptic facilitation mechanism of Aplysia. CONSTITUTION: The inhibitor of long-term synaptic facilitation comprises proteins which inhibits the long-term synaptic facilitation in sense-movement synapse of Aplysia by simultaneous over-expression and inhibition of ApCREB2(cyclic AMP-response element binding protein 2), wherein the inhibitor inhibits the expression of ApCREB2 in its transcription step.

Abstract translation: 目的：提供长期突触促进抑制剂，通过控制ApCREB2（环AMP反应元件结合蛋白2）的表达，有效抑制长期突触的促进，使其可用于长时间的研究 Aplysia的突触促进机制。 构成：长期突触促进的抑制剂包括通过同时过表达和抑制ApCREB2（环AMP应答元件结合蛋白2）抑制Aplysia意义上的突触促进的长期突触促进作用的蛋白质，其中抑制剂 抑制ApCREB2在其转录步骤中的表达。

公开(公告)号：KR1020040036059A

公开(公告)日：2004-04-30

申请号：KR1020020064846

申请日：2002-10-23

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  이진아

IPC: A61K38/16

Abstract: PURPOSE: Provided is a synaptic plasticity inhibitor which has 35 glutamine repeat units. It is useful for the study of pathological mechanism of neurodegenerative diseases, such as Huntington's disease. CONSTITUTION: A synaptic plasticity inhibitor is characterized by containing huntington(htt) protein which is capable of inhibiting synaptic plasticity by forming aggregates and having 35 or more glutamine repeat units at N-terminal of the huntington protein.

Abstract translation: 目的：提供具有35个谷氨酰胺重复单位的突触可塑性抑制剂。 对于亨廷顿氏病等神经变性疾病的病理机制的研究是有用的。 构成：突触可塑性抑制剂的特征在于含有能够通过形成聚集体并在亨廷顿蛋白N末端具有35个或更多个谷氨酰胺重复单元来抑制突触可塑性的亨廷顿蛋白（htt）蛋白。

公开(公告)号：KR1020070106121A

公开(公告)日：2007-11-01

申请号：KR1020060038615

申请日：2006-04-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  장동혁

IPC: C07K14/435 ,  C12N15/12

CPC classification number: G01N33/5058 ,  C07K14/43504

Abstract: A novel protein cofilin derived from Aplysia kurodai is provided to screen an active material for reducing cofilin-actin rod formation because cofilin-actin rod formation is associated with damage for strength and plasticity of synapse, thereby easily screening an active agent for prevention and treatment of neurodegenerative disease. A novel protein cofilin derived from Aplysia kurodai with binding activity to actin in a nerve cell has the amino acid sequence of SEQ ID NO:2. A gene encoding the cofilin has the nucleotide sequence of SEQ ID NO:1. A screening system of an active agent for prevention and treatment of neurodegenerative disease comprises the steps of: inserting the cofilin gene into an expression vector having an activation tag; transforming a nerve cell of Aplysia kurodai with the recombinant vector; culturing the transformed nerve cell in medium to overexpress the gene; adding candidate materials into the cultured nerve cell; and screening a material capable of reducing the formation of cofilin-actin rod structure.

Abstract translation: 提供衍生自黑腹果蝇的新型蛋白质cofilin，以筛选用于减少cofilin-肌动蛋白棒形成的活性物质，因为cofilin-肌动蛋白棒形成与突触强度和可塑性的损伤相关，从而容易地筛选活性剂以预防和治疗 神经变性疾病 衍生自神经细胞中与肌动蛋白结合活性的白色念珠菌的新型蛋白质椰子蛋白具有SEQ ID NO：2的氨基酸序列。 编码cofilin的基因具有SEQ ID NO：1的核苷酸序列。 用于预防和治疗神经变性疾病的活性剂的筛选系统包括以下步骤：将所述cofilin基因插入具有激活标签的表达载体中; 用重组载体转化黑腹果蝇神经细胞; 在培养基中培养转化的神经细胞以过表达基因; 将候选物质添加到培养的神经细胞中; 并筛选能够减少cofilin-肌动蛋白棒结构形成的材料。

公开(公告)号：KR100552401B1

公开(公告)日：2006-02-20

申请号：KR1020030019711

申请日：2003-03-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김형

IPC: C07K14/47

Abstract: 본 발명은 핵 또는 핵소체(nucleolus)로의 타겟팅에 관여하는 핵 국소화 신호 서열을 포함하는 단백질에 관한 것으로 상기 단백질은 서열번호: 1의 염기서열로 엔코딩되고 상기 국소화 신호 서열은 제85 내지 142번째 및 제355 내지 445번째 염기서열로부터 엔코딩되는 영역에 존재한다.
LAPS18, 핵, 핵소체, 국소화 신호 서열, 핵 국소화 신호 서열