-
公开(公告)号：KR101025707B1
公开(公告)日：2011-03-30
申请号：KR1020020073333
申请日：2002-11-23
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
Abstract: 본 발명은 사람 알파1-안티트립신(Alpha1-antitrypsin)을 발현하는 유전자를 성숙한(adult) 소에서 유래한 체세포에 도입 및 적중시키는 단계; 상기의 사람 알파1-안티트립신을 발현하는 외래 유전자가 도입 및 적중된 체세포를 이용하여 복제소를 생산하는 단계를 포함하는 형질전환 복제소의 생산방법 및 이러한 방법에 의해 생산된 알파-1 안티트립신을 발현하는 형질전환(transgenic) 복제소에 관한 것이다.
또한, 본 발명은 사람 알파1-안티트립신을 발현하는 유전자를 성숙한 소의 체세포에 도입 및 적중시키고, 상기 형질전환된 체세포를 이용하여 생산된 복제소의 유선으로부터 사람 알파1-안티트립신을 생산하는 방법에 관한 것이다.
사람 알파1-안티트립신, 체세포 복제, 형질전환 복제 소-
公开(公告)号：KR1020040016426A
公开(公告)日：2004-02-21
申请号：KR1020030056629
申请日：2003-08-14
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: A01K67/0273 , A01K67/0275 , A01K2227/101 , A01K2267/01 , C12N9/6456 , C12N15/8509 , C12N15/8771 , C12N2830/008
Abstract: PURPOSE: A transgenic cloned cow producing human prourokinase and a method for producing the same are provided, thereby expressing human prourokinase from the mammary gland of the transgenic cloned cow, and easily separating it from milk. CONSTITUTION: Provided is a nucleus transplanted ovum wherein a nucleus of a somatic cell derived from a cow and containing a human prourokinase gene is fused with a nucleus removed ovum derived from cow. The nucleus transplanted ovum is SNU-B1(KCTC 10286BP). A method for producing the nucleus transplanted ovum comprises the steps of: (a) introducing a human prourokinase gene into the somatic cell derived from cow to prepare a nucleus transferring cell; (b) removing a cumulus cell and cytoplasm from a ovum of a cow to prepare a nucleus receiving ovum; and (c) transplanting the nucleus transferring cell into the nucleus receiving ovum and fusing them.
Abstract translation: 目的：提供产生人类原尿激酶的转基因克隆牛及其生产方法，从而从转基因克隆牛的乳腺中表达人类原尿激酶，并且容易地将它与牛分离。 构成：提供了一种移植卵的细胞核，其中源自牛的体细胞细胞核并含有人类原尿激酶基因与从牛获得的去除卵的细胞核融合。 移植的卵细胞为SNU-B1（KCTC 10286BP）。 用于产生移植卵的细胞的方法包括以下步骤：（a）将人类原尿激酶基因导入来自牛的体细胞，制备细胞核转移细胞; （b）从牛的卵中除去卵丘细胞和细胞质以制备接受卵的细胞核; 和（c）将细胞核转移细胞移植到接受卵子的细胞核中并融合它们。
-
公开(公告)号：KR1020040045528A
公开(公告)日：2004-06-02
申请号：KR1020020073333
申请日：2002-11-23
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/8771 , A01K67/0273 , A01K2227/101 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2510/00 , C12N2510/02 , C12N2517/04
Abstract: PURPOSE: Transgenic cloned cow producing human alpha1-antitrypsin and a method for producing the same are provided, thereby economically and efficiently producing biological medicines from the cloned animals. CONSTITUTION: The method for producing the transgenic cloned cow producing human alpha1-antitrypsin comprises the steps of: (a) preparing nucleus donor cells by transferring a gene encoding human alpha1-antitrypsin to somatic cell lines collected from cow; (b) preparing matured nucleus recipient ova collected from cow by removing oocytes from nucleus recipient ova of cow and removing cytoplasm including the first polar body; and (c) transferring the nucleus donor cells to the matured nucleus recipient ova and fusing them, wherein the somatic cells are collected from matured cow; and the nucleus transplanted ovum is SNU-B3(KCTC 10356BP).
Abstract translation: 目的：提供转基因克隆牛产生人α1-抗胰蛋白酶及其制备方法，从而经济有效地从克隆的动物产生生物药物。 构成：生产人α1-抗胰蛋白酶的转基因克隆牛的方法包括以下步骤：（a）通过将编码人α1-抗胰蛋白酶的基因转移到从母牛收集的体细胞系中来制备核供体细胞; （b）通过从牛的核受体卵中除去卵母细胞并除去包括第一极体的细胞质来制备从牛采集的成熟的细胞核受体ova; 和（c）将核供体细胞转移到成熟的细胞核受体ova并使其融合，其中从成熟的牛收集体细胞; 移植的卵细胞为SNU-B3（KCTC 10356BP）。
-
Patent Agency Ranking
公开(公告)号：KR100546819B1
公开(公告)日：2006-01-26
申请号：KR1020030056629
申请日：2003-08-14
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: A01K67/0273 , A01K67/0275 , A01K2227/101 , A01K2267/01 , C12N9/6456 , C12N15/8509 , C12N15/8771 , C12N2830/008
Abstract: 본 발명은 사람 프로유로키나제를 발현하는 유전자를 성숙한 소 유래의 체세포에 도입 및 적중시키는 단계; 및 사람 프로유로키나제를 발현하는 외래 유전자가 도입 및 적중된 체세포를 이용하여 복제 소를 생산하는 단계를 포함하는 사람 프로유로키나제를 생산하는 형질전환 복제 소의 생산 방법 및 이 방법에 의해 생산된 형질전환 복제 소에 관한 것이다.
또한, 본 발명은 유전자 도입 및 적중 기술과 체세포 복제 기술을 접목하여 사람 프로유로키나제를 용이하게 얻는 방법에 관한 것으로 더 구체적으로, 성숙한 소에서 유래한 체세포에 사람 프로유로키나제를 발현하는 유전자를 도입 및 적중시키고, 이를 체세포 복제하여 유선에서 사람 프로유로키나제를 발현하는 형질전환 복제 소를 생산함으로써 소의 우유로부터 사람 프로유로키나제를 수득할 수 있는 방법에 관한 것이다.
형질전환 복제소, 체세포 복제, 프로유로키나제,인간유용단백질-
公开(公告)号：KR1020040060348A
公开(公告)日：2004-07-06
申请号：KR1020020087129
申请日：2002-12-30
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/07
CPC classification number: C12N5/0604 , A01K67/0273 , A01K2227/101 , A01K2227/106 , C12N15/8771 , C12N15/8776 , C12N2500/44 , C12N2501/998 , C12N2517/00
Abstract: PURPOSE: A medium composition and culture method for improving preimplantation development of clone embryo are provided, thereby effectively inhibiting apoptosis of blastomere to improve preimplantation development of clone embryo, thereby improving the production yield of health clone embryo. CONSTITUTION: The medium composition for in vitro culturing the nucleus transferring somatic cell for preparing clone embryo contains 1-25 micromole of antibiotics such as beta-mercaptoethanol, and 1-10 microgram/milliliter of an oxidized nitrogen removing factor such as hemoglobin. The method for in vitro culturing the clone embryo in the medium composition comprises the steps of: (a) in vitro culturing the nucleus transferring somatic cell in the in vitro culturing medium composition containing antibiotics; (b) transplanting the nucleus of the nucleus transferring somatic cell to a nucleus receiving egg of which genetic materials are removed to prepare somatic cell clone embryo; and (c) in vitro culturing the somatic cell clone embryo in the in vitro culturing medium composition containing antibiotics and oxidized nitrogen removing factor.
Abstract translation: 目的：提供一种改善克隆胚胎植入前发育的培养基和培养方法，有效抑制卵裂球细胞凋亡，改善克隆胚胎植入前的发育，提高了克隆胚胎的产量。 构成：用于体外培养转运体细胞以制备克隆胚胎的培养基组合物含有1-25微摩尔的抗生素如β-巯基乙醇和1-10微克/毫升的氧化除氮因子如血红蛋白。 在培养基组合物中体外培养克隆胚胎的方法包括以下步骤：（a）体外培养在含有抗生素的体外培养培养基组合物中转运体细胞的细胞核; （b）将转移体细胞的细胞核移植到接受蛋的细胞核，其中遗传物质被去除以制备体细胞克隆胚胎; 和（c）在含有抗生素和氧化除氮因子的体外培养培养基组合中体外培养体细胞克隆胚胎。
-
公开(公告)号：KR1020040047313A
公开(公告)日：2004-06-05
申请号：KR1020020075471
申请日：2002-11-29
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/8771 , A01K67/0273 , A01K2227/101 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2510/00 , C12N2510/02 , C12N2517/02
Abstract: PURPOSE: Transgenic cloned cow producing human lactoferricin and a method for producing the same are provided, thereby economically and efficiently producing biological medicines from the cloned animals and easily obtaining human lactoferricin from the milk of cloned animals. CONSTITUTION: The method for producing the transgenic cloned cow producing human lactoferricin comprises the steps of: (a) preparing nucleus donor cells by transferring a gene encoding human lactoferricin to somatic cell lines collected from cow; (b) preparing matured nucleus recipient ova collected from cow by removing oocytes from nucleus recipient ova of cow and removing cytoplasm including the first polar body; and (c) transferring the nucleus donor cells to the matured nucleus recipient ova and fusing them, wherein the somatic cells are collected from matured cow; and the nucleus transplanted ovum is SNU-B2(KCTC 10355BP).
Abstract translation: 目的：提供转基因克隆牛产生人乳铁蛋白及其生产方法，从而从克隆的动物中经济有效地生产生物药物，并且容易地从克隆动物的乳中获得人乳铁蛋白。 构成：生产人乳铁蛋白的转基因克隆牛的方法包括以下步骤：（a）通过将编码人乳铁蛋白的基因转移到从母牛收集的体细胞系中来制备核供体细胞; （b）通过从牛的核受体卵中除去卵母细胞并除去包括第一极体的细胞质来制备从牛采集的成熟的细胞核受体ova; 和（c）将核供体细胞转移到成熟的细胞核受体ova并使其融合，其中从成熟的牛收集体细胞; 移植的卵细胞为SNU-B2（KCTC 10355BP）。
-
公开(公告)号：KR100975350B1
公开(公告)日：2010-08-12
申请号：KR1020020075471
申请日：2002-11-29
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
Abstract: 본 발명은 사람 락토페리신을 발현하는 유전자를 성숙한(adult) 소에서 유래한 체세포에 도입 및 적중시키는 단계; 상기의 사람 락토페리신을 발현하는 외래 유전자가 도입 및 적중된 체세포를 이용하여 복제소를 생산하는 단계를 포함하는 형질전환 복제소의 생산방법 및 이러한 방법에 의해 생산된 락토페리신을 발현하는 형질전환(transgenic) 복제소에 관한 것이다.
또한, 본 발명은 사람 락토페리신을 발현하는 유전자를 성숙한 소의 체세포에 도입 및 적중시키고, 상기 형질전환된 체세포를 이용하여 생산된 복제소의 유선으로부터 사람 락토페리신을 생산하는 방법에 관한 것이다.
사람 알파1-안티트립신, 체세포 복제, 형질전환 복제 소-
公开(公告)号：KR1020040101793A
公开(公告)日：2004-12-03
申请号：KR1020030033582
申请日：2003-05-27
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N5/16
CPC classification number: C12N15/8771 , A01K67/0273 , A01K2227/101 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2517/00
Abstract: PURPOSE: Transgenic cloned cow producing human erythropoietin and a method for producing the same transgenic cloned cow are provided, which method allows economic and effective production of various biomedicines, and facilitates production of human erythropoietin in cow's milk. CONSTITUTION: The nuclear transfer embryo produced by a fusion of a cow-derived enucleated oocyte with a nucleus of a cow-derived somatic cell transfected with a gene encoding human erythropoietin is provided, wherein the cow is an adult; the nuclear transfer embryo is SNU-B4(KCTC 10450BP). The method for producing a transgenic cloned cow expressing human erythropoietin in the mammary gland comprises the steps of: (a) introducing a gene encoding human erythropoietin into a cow-derived somatic cell line to prepare a nuclear donor cell; (b) removing cumulus cells surrounding a recipient oocyte and then the cytoplasm including the first polar body from the oocyte to prepare a cow-derived enucleated recipient oocyte; (c) transferring the transfected nuclear donor cell into the enucleated recipient oocyte and carrying out cell fusion to generate nuclear transfer embryos; and (d) transplanting the nuclear transfer embryo into a surrogate mother cow to produce live offspring.
Abstract translation: 目的：提供转基因克隆的牛产生人促红细胞生成素和生产相同转基因克隆牛的方法，该方法允许经济有效地生产各种生物医学，并促进牛奶中人促红细胞生成素的产生。 构成：提供由牛衍生的去核卵母细胞与用编码人促红细胞生成素的基因转染的牛衍生的体细胞的细胞核融合而产生的核转移胚，其中，牛是成年人; 核转移胚是SNU-B4（KCTC 10450BP）。 用于产生在乳腺中表达人促红细胞生成素的转基因克隆牛的方法包括以下步骤：（a）将编码人促红细胞生成素的基因导入牛衍生的体细胞系以制备核供体细胞; （b）去除卵母细胞周围的卵丘细胞，然后从卵母细胞中除去包含第一极体的细胞质，以制备牛衍生的去核受体卵母细胞; （c）将转染的核供体细胞转移到去核的受体卵母细胞中并进行细胞融合以产生核转移胚胎; 和（d）将核转移胚胎移植到替代母牛中以产生活的后代。
-
-
-
-
-
-
-
Search Results
8
records
(took 0.023 seconds)
