公开(公告)号：WO1995017679A1

公开(公告)日：1995-06-29

申请号：PCT/US1994014716

申请日：1994-12-19

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WONG, Martin ,  FINLEY, David, M. ,  RAMP, John, M. ,  BOLTINGHOUSE, Gary, L., Jr. ,  SHAFFAR, Mark, R. ,  STROUPE, Stephen, D. ,  BRACKETT, John, M.

IPC: G01N33/84

CPC classification number: G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

Abstract: Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

Abstract translation: 检测样品中存在的铅，包括以下步骤：（a）将铅回收剂加入到含有铅的测定溶液中; （b）向测定溶液中加入在铅存在下被抑制的二硫化物酶; 和（c）将二硫键的活性与样品中的铅的量相关联。 铅回收剂，优选铅螯合剂，提高了测定的灵敏度和准确性，使得该测定可以容易地自动化，以便使用市售的自动化系统检测全血中的铅。

公开(公告)号：WO1993007298A1

公开(公告)日：1993-04-15

申请号：PCT/US1992008035

申请日：1992-09-22

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  JOU, Yi-Her ,  STROUPE, Stephen, D. ,  ADAMCZYK, Janina ,  SAFFORD, John

IPC: C12Q01/70

CPC classification number: G01N33/538 ,  G01N33/54366 ,  G01N33/56983 ,  G01N33/57473 ,  Y10S435/969 ,  Y10S435/971 ,  Y10S436/811 ,  Y10S436/823

Abstract: The present invention includes novel rubella assays employing a Rubella virus capture reagent and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing Rubella antibody may be contacted with the capture reagent to form a capture reagent/analyte complex. The complex is then contacted to the positively charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex.

Abstract translation: 本发明包括使用风疹病毒捕获试剂的新型风疹试验和含有包含聚合物阳离子物质的反应位点的固相材料。 怀疑含有风疹抗体的测试样品可以与捕获试剂接触以形成捕获试剂/分析物复合物。 然后将络合物与带正电的固相接触以吸引，附着和固定捕获试剂/分析物复合物。

公开(公告)号：WO1992015709A1

公开(公告)日：1992-09-17

申请号：PCT/US1992001572

申请日：1992-02-28

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  STROUPE, Stephen, D.

IPC: C12Q01/68

CPC classification number: C12Q1/6825 ,  B82Y35/00 ,  G01N33/53 ,  G01N33/54373

Abstract: Methods and test kits for detecting the presence of an analyte in a test sample on a molecule-by-molecule basis by using scanning probe microscopy, in which the test sample suspected of containing the analyte of interest is exposed to a test piece to which an analyte specific substance has been attached, and the test piece is scanned by scanning probe microscopy to determine the presence or absence of the analyte.

Abstract translation: 用于通过使用扫描探针显微镜在分子基础上检测测试样品中分析物的存在的方法和测试试剂盒，其中将怀疑含有感兴趣分析物的测试样品暴露于试验片， 已经附着分析物特异性物质，并通过扫描探针显微镜扫描测试片以确定分析物的存在或不存在。

公开(公告)号：WO1992021770A1

公开(公告)日：1992-12-10

申请号：PCT/US1992002980

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  ADAMCZYK, Janina ,  BERRY, Daniel, S. ,  FICO, Rosario ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: C12Q01/00

CPC classification number: G01N33/538

Abstract: This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent.

Abstract translation: 本发明提出了使用捕获试剂的新型测定方法，其包括通常与聚合物阴离子物质结合的第一结合构件，以及含有含有至少约2％氮含量的聚合阳离子物质的反应位点的固相材料。 在一个实施方案中，怀疑含有感兴趣的分析物的测试样品可与捕获试剂接触以形成带电的捕获试剂/分析物复合物。 然后将络合物与相反电荷的固相接触以吸引，附着和固定捕获试剂/分析物复合物。 使用具有至少约2％的氮含量（不包括抗衡离子）的聚阳离子物质来制备适当带电的固相，提供可以进行更大程度操作的固相，例如多次洗涤 ，而不失去吸引和保留捕获试剂的能力。

公开(公告)号：WO1992021769A1

公开(公告)日：1992-12-10

申请号：PCT/US1992002979

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  ADAMCZYK, Janina ,  BERRY, Daniel, S. ,  FICO, Rosario ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: C12Q01/00

CPC classification number: G01N33/54393 ,  G01N33/538 ,  G01N33/54353 ,  G01N33/544

Abstract: The present invention includes novel assays and reagents using a capture reagent, involving a specific binding member attached to a polymeric anionic substance, and a solid phase material containing a capture or reaction zone including a polymeric cationic substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, sandwich, competitive and indirect assays can be performed. Preferably, the indicator reagent includes a nonspecific binding blocker to reduce the nonspecific binding between the indicator reagent and the solid phase, without interfering with the reaction between the capture reagent and the solid phase. Suitable nonspecific binding blockers include unbound or free polyanionic materials such as dextran sulfate, heparin, carboxymethyl dextran, carboxymethyl cellulose, pentosan polysulfate, inositol hexasulfate or beta -cyclodextrin sulfate.

Abstract translation: 本发明包括使用捕获试剂，包括连接到聚合阴离子物质的特异性结合成员的新型测定和试剂，以及含有包含聚合阳离子物质的捕获或反应区的固相材料。 怀疑含有感兴趣的分析物的测试样品可以与捕获试剂接触以形成带电的捕获试剂/分析物复合物。 然后将络合物与相反电荷的固相接触以吸引，附着和固定捕获试剂/分析物复合物。 使用适当的指示剂，夹心，可以进行竞争性和间接测定。 优选地，指示剂试剂包括非特异性结合阻断剂，以减少指示剂与固相之间的非特异性结合，而不干扰捕获试剂和固相之间的反应。 合适的非特异性结合阻断剂包括未结合或游离的聚阴离子材料，例如硫酸葡聚糖，肝素，羧甲基葡聚糖，羧甲基纤维素，戊聚糖多硫酸酯，六水合硫酸肌醇或硫酸β-环糊精。

公开(公告)号：WO1995024651A1

公开(公告)日：1995-09-14

申请号：PCT/US1995002897

申请日：1995-03-06

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  KIM, Young, Ran ,  STROUPE, Stephen, D.

IPC: G01N33/72

CPC classification number: G01N33/5094 ,  G01N33/721 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/25375

Abstract: A cyanide-free method and reagent for determining the concentration of total hemoglobin in a whole blood sample accurately in less than 10 seconds comprising a ligand selected from the group consisting of imidazole, imidazole derivatives, N-hydroxyacetamide, N-hydroxyl amine, pyridine, oxazole, thiazole, pyrazole, pyrimidine, purine, quinoline, and isoquinoline, and a surfactant with strong erythrolytic capability selected from the group consisting of lauryl dimethylamine oxide and octylphenoxy polyethoxyethanol. The reagent pH is adjusted to about 11 to about 14. Rapid mixing of the reagent with a blood sample leads to the formation of a stable chromogen whose absorbance can be measured between 540 and 550 nm. The cyanide-free reagent is ideal for use on an automated high through-put clinical hematology analyzer.

Abstract translation: 一种不含氰化物的方法和用于在小于10秒内精确测定全血样品中总血红蛋白浓度的试剂，其包含选自咪唑，咪唑衍生物，N-羟基乙酰胺，N-羟基胺，吡啶， 恶唑，噻唑，吡唑，嘧啶，嘌呤，喹啉和异喹啉，以及选自月桂基二甲基氧化胺和辛基苯氧基聚乙氧基乙醇的具有强的红细胞能力的表面活性剂。 将试剂pH调节至约11至约14.试剂与血液样品的快速混合导致形成稳定的色原，其吸光度可在540至550nm之间测量。 无氰试剂非常适用于自动化高通量临床血液分析仪。

公开(公告)号：WO1992021975A1

公开(公告)日：1992-12-10

申请号：PCT/US1992002997

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  KLINE, Steven ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: G01N33/536

CPC classification number: G01N33/543 ,  G01N33/532 ,  G01N33/545 ,  G01N33/56988 ,  G01N33/57473 ,  G01N33/58 ,  G01N33/743 ,  G01N33/76 ,  G01N33/9453

Abstract: The present invention includes novel digoxin assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex.

Abstract translation: 本发明包括采用捕获试剂的新型地高辛测定法，其涉及与聚合阴离子物质缀合的第一结合成员，以及含有含有至少约2％氮含量的聚合阳离子物质的反应位点的固相材料。 怀疑含有感兴趣的分析物的测试样品可以与捕获试剂接触以形成带电的捕获试剂/分析物复合物。 然后将络合物与相反电荷的固相接触以吸引，附着和固定捕获试剂/分析物复合物。

公开(公告)号：WO1992012255A1

公开(公告)日：1992-07-23

申请号：PCT/US1991009714

申请日：1991-12-23

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  KHALIL, Omar, S. ,  GENGER, Kevin, R. ,  COTTER, Stephen, M. ,  JOU, Yi-Her ,  ABUNIMEH, Noman, A. ,  HILTIBRAND, Robert, G. ,  STROUPE, Stephen, D.

IPC: C12Q01/00

CPC classification number: G01N33/582 ,  C12Q1/6816 ,  G01N33/54313 ,  G01N33/54326

Abstract: Assays for the determination of multiple analytes which may be present in a test sample by using different short-lived and long-lived chemiluminescent labels and integrating the generated chemiluminescence signal and time-discriminating the short-lived and the long-lived components of the signal generated. Also provided are assay kits which contain these short-lived and long-lived chemiluminescent compounds.

Abstract translation: 通过使用不同的短寿命和长寿命的化学发光标记并整合生成的化学发光信号和时间识别信号的短寿命和长寿命成分，来测定可能存在于测试样品中的多种分析物 产生。 还提供了含有这些短寿命和长寿命化学发光化合物的测定试剂盒。

公开(公告)号：WO1996027785A1

公开(公告)日：1996-09-12

申请号：PCT/US1996002845

申请日：1996-02-28

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WU, Xiaomao ,  JOU, Yi-Her ,  HUANG, Tung-Ming ,  STROUPE, Stephen, D. ,  LI, Conan, K., N. ,  ALCOCK, Alan, J. ,  GUTIERREZ, Edward, J.

IPC: G01N21/76

CPC classification number: G01N21/05 ,  G01N21/76 ,  G01N2021/0346

Abstract: A method and article for conducting a chemiluminescent reaction in which a reaction conduit is provided having a unique configuration for enhanced chemiluminescent signal production and detection. The conduit is preferably provided with a plurality of folds defining multiple substantially different flow paths for reactants flowing therethrough. The method and article are suitable for use in diagnostic assays employing chemiluminescent labels.

Abstract translation: 用于进行化学发光反应的方法和制品，其中提供具有用于增强的化学发光信号产生和检测的独特配置的反应导管。 导管优选地设置有多个折痕，其限定用于流过其中的反应物的多个基本不同的流动路径。 该方法和制品适用于采用化学发光标记的诊断测定。

公开(公告)号：WO1995025282A1

公开(公告)日：1995-09-21

申请号：PCT/US1995003168

申请日：1995-03-14

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HILTIBRAN, Robert, G. ,  JOU, Yi-Her ,  STROUPE, Stephen, D. ,  KLINE, Steven, J. ,  SCHULTZ, Steven, G.

IPC: G01N33/538

CPC classification number: G01N33/538

Abstract: This invention presents novel reagents, separation techniques and assay procedures which allow both the indicator and the capture reagents to be in solution to avoid problems of slowed reaction kinetics. The separation procedure involves a soluble capture reagent, comprising a specific binding member attached to a charged substance, and a insoluble solid phase that is oppositely charged with respect to the charged substance included in the capture reagent. A test sample suspected of containing the analyte of interest is mixed with the capture reagent to form a charged capture reagent/analyte complex. The reaction mixture is contacted to the oppositely charged solid phase to attract, attach, and separate the capture reagent/analyte complexes and any unreacted capture reagent from the reaction mixture. With an appropriate indicator reagent, i.e., a second specific binding member which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed.

Abstract translation: 本发明提出新型试剂，分离技术和测定方法，其允许指示剂和捕获试剂在溶液中以避免反应动力学减慢的问题。 分离方法包括可溶性捕获试剂，其包含与带电物质连接的特异性结合成员，以及相对于包含在捕获试剂中的带电物质相反带电的不溶性固相。 将怀疑含有感兴趣分析物的测试样品与捕获试剂混合以形成带电的捕获试剂/分析物复合物。 将反应混合物与相反电荷的固相接触以吸引，附着并将捕获试剂/分析物复合物和任何未反应的捕获试剂从反应混合物分离。 使用合适的指示剂，即与能够产生可检测信号的标签缀合的第二特异性结合成员，可以进行夹心和竞争性测定。