Abstract:
The invention relates to a method for the production of at least one microbial metabolic product having at least 3 C atoms or at least 2 C atoms and at least one N atom by means of sugar-based microbial fermentation. The inventive method involves the following steps: a) producing a sugar-containing liquid medium having a monosaccharide content of more than 20 weight percent from a starch source, wherein the sugar-containing liquid medium also comprises nonstarch-containing solid components of the starch source; b) fermenting the sugar-containing liquid medium for the production of the metabolic product(s) and c) separating or isolating at least one metabolic product from the fermentation broth, wherein the microorganism strain producing the desired metabolic product(s) is cultivated with the sugar-containing liquid medium, which is obtained by: a1) milling the starch source and a2) liquefying the ground material in an aqueous fluid in the presence of at least one starch liquefying enzyme and subsequently saccharifyng the resulting liquid using at least one saccharifying enzyme, wherein at least part of the ground material is liquefied into an aqueous liquid by continuous or discontinuous addition.
Abstract:
The invention relates to a method for the production of at least one microbial metabolic product having at least 3 C atoms or at least 2 C atoms and at least one N atom by means of sugar-based microbial fermentation. The inventive method involves the following steps: a) producing a sugar-containing liquid medium having a monosaccharide content of more than 20 weight percent from a starch source, wherein the sugar-containing liquid medium also comprises nonstarch-containing solid components of the starch source; b) fermenting the sugar-containing liquid medium for the production of the metabolic product(s) and c) separating or isolating at least one metabolic product from the fermentation broth, wherein the microorganism strain producing the desired metabolic product(s) is cultivated with the sugar-containing liquid medium, which is obtained by: a1) milling the starch source and a2) liquefying the ground material in an aqueous fluid in the presence of at least one starch liquefying enzyme and subsequently saccharifyng the resulting liquid using at least one saccharifying enzyme, wherein at least part of the ground material is liquefied into an aqueous liquid by continuous or discontinuous addition.
Abstract:
The invention relates to a method for the fermentative production of methionine, to a method for the insolation of the methionine thus formed, organic substances containing methionine occurring during insulation, to the use thereof in the production of animal food or animal food complements, in addition to the use of insolated methionine in the production of human or animal food or human or animal food complements.
Abstract:
The invention relates to a process for the production of at least one microbial metabolite having at least 3 carbon atoms or at least 2 carbon atoms and at least 1 nitrogen atom by means of sugar-based microbial fermentation, comprising: a) the preparation of a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight from a starch feedstock, the sugar-containing liquid medium also comprising non-starchy solid constituents of the starch feedstock; b) the fermentation of the sugar-containing liquid medium for the production of the metabolite(s); and c) depletion or isolation of at least one metabolite from the fermentation liquor, wherein a microorganism strain which produces the desired metabolite(s) is cultivated with the sugar-containing liquid medium, said liquid medium being obtained by: a1) milling the starch feedstock; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, where at least some of the millbase is liquefied by continuous or batchwise addition to the aqueous liquid.
Abstract:
The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: a1) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; a2) the grinding material is suspended in an aqueous liquid and said grinding material contained in the aqueous liquid is liquefied in the presence of at least one enzyme which liquefies starch, an aqueous substance which contains dextrin (1), which contains at least one part of the non-starch containing solid components of the starch source, is obtained; and b) the aqueous substance which contains dextrin (1) is used in fermentation for cultivating a micro-organism which is capable of over-producing the organic compounds; enzymes, which hydrolyse the dextrin into monosaccharides, are not added or are added in an amount of less than 0.001 wt. %, in relation to the total weight of the used starch source.
Abstract:
La invencion se refiere a un proceso para la produccion de al menos un metabolito microbiano con al menos 3 atomos de carbono, o al menos 2 atomos de carbono y al menos 1 atomo de nitrogeno mediante fermentacion microbiana basada en azucar, que comprende: a) la preparacion de un medio liquido que contiene azucar con un contenido de monosacarido de mas del 20% en peso proveniente de un alimento de almidon, en donde el medio liquido que contiene azucar tambien comprende constituyentes solidos distintos de almidon del alimento de almidon; b) la fermentacion del medio liquido que contiene azucar para la produccion del (os) metabolito(s); y c) la deplecion o el aislamiento de al menos un metabolito a partir del licor de fermentacion, en donde una cepa de microorganismo que produce el(os) metabolito(s) de interes se cultiva en el medio liquido que contiene azucar, en donde el medio liquido se obtiene por: a1)molienda del aliento de almidon; y a2) licuefaccion de la base de molienda en un liquido acuoso en presencia de al menos una enzima que licua el almidon, seguido de sacarificacion con al menos una enzima sacarificante, en donde al menos parte de la base de molienda se licua por adicion continua o en tandas al liquido acuoso.
Abstract:
Preparation of peptidoglycan (I) or its derivatives from gram-positive bacteria comprising isolating (I) from a biomass (A) containing the gram-positive bacteria, where the isolation is carried out in partial stages comprising incubating (A) with a solution (containing at least one detergent) at 20-90[deg]C to obtain a mixture (II) containing solvents, detergents, soluble components, (I) and optionally further solid components, is new. Independent claims are also included for: (1) (I) and its derivative obtained by the method; and (2) a copolymer comprising (I) or one of its derivatives as a monomer unit.
Abstract:
The invention relates to a process for the production of at least one microbial metabolite having at least 3 carbon atoms or at least 2 carbon atoms and at least 1 nitrogen atom by means of sugar-based microbial fermentation, comprising: a) the preparation of a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight from a starch feedstock, the sugar-containing liquid medium also comprising non-starchy solid constituents of the starch feedstock; b) the fermentation of the sugar-containing liquid medium for the production of the metabolite(s); and c) depletion or isolation of at least one metabolite from the fermentation liquor, wherein a microorganism strain which produces the desired metabolite(s) is cultivated with the sugar-containing liquid medium, said liquid medium being obtained by: a1) milling the starch feedstock; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, where at least some of the millbase is liquefied by continuous or batchwise addition to the aqueous liquid.
Abstract:
The invention relates to a process for enriching trehalose from solutions, in which the enrichment is performed using an adsorbent, in which the adsorbent is an aluminosilicate. Preferably, the aluminosilicate is a zeolite. The invention further relates to the enrichment and purification of trehalose from fermentation broths, in particular as a coupled product of production by fermentation of other products of value.