公开(公告)号：CA2745183A1

公开(公告)日：2010-06-10

申请号：CA2745183

申请日：2009-12-01

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R

IPC: C07F5/00 ,  C07F11/00 ,  C07F13/00 ,  C07F15/00

Abstract: A complex containing a transition metal ion and a plurality of donor ligands each of which is fully coordinated to the transition metal ion and is either a nitrogen donor ligand or a cyclometalated donor ligand, such that at least one of the donor ligands is a cyclometalated donor ligand bears one or more reactive groups connected to at least one of the donor ligands through a linker that includes a chain of four or more atoms. The linker offers advantages that make the complex particularly effective in labeling biomolecules and in multiplex analyses.

公开(公告)号：CA2736107A1

公开(公告)日：2010-03-11

申请号：CA2736107

申请日：2009-09-02

Applicant: BIO RAD LABORATORIES

Inventor： ROWELL CRAIG ,  PANATTONI CORY ,  BERKELMAN THOMAS R ,  CATER SEAN ,  PETERSEN SHANE ,  OLECH LEE ,  YANG XUEMEI

IPC: G01N33/558 ,  G01N33/559 ,  G01N33/561

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：CA2661663A1

公开(公告)日：2008-03-06

申请号：CA2661663

申请日：2007-08-27

Applicant: BIO RAD LABORATORIES

Inventor： VOLKOVA KATERYNA D ,  KOVALSKA VLADYSLAVA B ,  LOSYTSKYY MYKHAYLO YU ,  YARMOLUK SERGIY M ,  BERKELMAN THOMAS R

IPC: G03F9/00

Abstract: Squaraine dyes are incorporated into a separation medium in which protein or polypeptide mixtures are separated, which medium also contains a deterge nt that forms a complex with the polypeptides or proteins. The dyes, upon ex citation in the separation medium, exhibit a heretofore unrecognized selecti vity in their fluorescent emissions by emitting a signal only when the dye m olecules are associated with complexes of protein (or polypeptide) and deter gent molecules, despite the additional presence of dyes in the bulk of the s eparation medium. The dyes are thus able to indicate the presence and locati ons of proteins or polypeptides in the separation medium without the need fo r removing unassociated dyes from the medium.

公开(公告)号：CA2592159A1

公开(公告)日：2006-08-03

申请号：CA2592159

申请日：2005-12-08

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R

IPC: G01N33/00 ,  C07F9/24

Abstract: Protein dyes whose molecular structure is that of a coumarin moiety coupled to a quaternary ammonium heterocycle through a vinyl or polyvinyl linkage demonstrate the ability to associate with proteins in a non-covalent, non-specific manner at low pH, where the associated form displays a significantly higher fluorescence emission than the unassociated form. This makes the dyes useful as selective labels for proteins at the low pH and eliminates the need for the removal of extraneous components from the medium in which the proteins reside prior to detection.

公开(公告)号：CA2793179C

公开(公告)日：2015-07-14

申请号：CA2793179

申请日：2011-03-09

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R ,  MARTIN LISANDRA ,  JONES RHIANNON ,  WALKER JOHN II

IPC: C07D255/02 ,  B01D11/04 ,  B01D15/08 ,  G01N21/76 ,  G01N27/00 ,  G01N27/447 ,  G01N33/52

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

公开(公告)号：CA2592159C

公开(公告)日：2012-07-24

申请号：CA2592159

申请日：2005-12-08

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R

IPC: G01N33/00 ,  C07F9/24

Abstract: Protein dyes whose molecular structure is that of a coumarin moiety coupled to a quaternary ammonium heterocycle through a vinyl or polyvinyl linkage demonstrate the ability to associate with proteins in a non-covalent, non-specific manner at low pH, where the associated form displays a significantly higher fluorescence emission than the unassociated form. This makes the dyes useful as selective labels for proteins at the low pH and eliminates the need for the removal of extraneous components from the medium in which the proteins reside prior to detection.

公开(公告)号：AU2011227637A1

公开(公告)日：2012-10-04

申请号：AU2011227637

申请日：2011-03-09

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R ,  MARTIN LISANDRA ,  JONES RHIANNON ,  WALKER II JOHN

IPC: C07D255/02

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

公开(公告)号：DE07814469T1

公开(公告)日：2009-09-03

申请号：DE07814469

申请日：2007-08-27

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R ,  YARMOLUK SERGIY M ,  KOVALSKA VLADYSLAVA B ,  LOSYTSKYY MYKHAYLO YU ,  VOLKOVA KATERYNA D

IPC: G03F9/00

公开(公告)号：AU2011227637B2

公开(公告)日：2014-05-08

申请号：AU2011227637

申请日：2011-03-09

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R ,  MARTIN LISANDRA ,  JONES RHIANNON ,  WALKER II JOHN

IPC: C07D255/02

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

公开(公告)号：CA2793179A1

公开(公告)日：2011-09-22

申请号：CA2793179

申请日：2011-03-09

Applicant: BIO RAD LABORATORIES

Inventor： BERKELMAN THOMAS R ,  MARTIN LISANDRA ,  JONES RHIANNON ,  WALKER JOHN II

IPC: C07D255/02 ,  B01D11/04 ,  B01D15/08 ,  G01N21/76 ,  G01N27/00 ,  G01N27/447 ,  G01N33/52

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.