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    MOLECULAR CYTOMETRY
    2.
    发明专利
    MOLECULAR CYTOMETRY 未知

    公开(公告)号：CA2913806A1

    公开(公告)日：2014-12-04

    申请号：CA2913806

    申请日：2014-05-22

    Applicant: FLUIDIGM CANADA INC

    Inventor： BANDURA DMITRY ,  BARANOV VLADIMIR ,  LOBODA ALEXANDER ,  ORNATSKY OLGA ,  TANNER SCOTT D

    IPC: G01N27/62 ,  G01N33/483

    Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

    LYOPHILIZED ANTIBODY PANEL
    3.
    发明专利
    LYOPHILIZED ANTIBODY PANEL 未知

    公开(公告)号：CA3134973A1

    公开(公告)日：2020-10-01

    申请号：CA3134973

    申请日：2020-03-27

    Applicant: FLUIDIGM CANADA INC

    Inventor： LI STEPHEN K H ,  MAJONIS DANIEL ,  BARANOV VLADIMIR ,  ORNATSKY OLGA ,  ALLO BEDILU ,  BANDURA DMITRY ,  THOM COLIN ,  STELZER GREG ,  LOBODA ALEXANDER

    IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

    Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

    SYSTEMS, METHODS AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MASS SPECTROMETRY
    6.
    发明专利
    SYSTEMS, METHODS AND COMPOSITIONS FOR SIMULTANEOUS DETECTION OF RNA AND PROTEIN BY MASS SPECTROMETRY 未知

    公开(公告)号：CA3029183A1

    公开(公告)日：2017-01-05

    申请号：CA3029183

    申请日：2016-06-29

    Applicant: FLUIDIGM CANADA INC

    Inventor： ORNATSKY OLGA

    IPC: C07H19/00 ,  C07H21/00 ,  C07H21/02

    Abstract: In certain embodiments, a method of detection includes providing cells labeled with a first element tag bound to a target RNA sequence and a second element tag bound to a target protein. The method may further include ionizing a cell, or a portion thereof, to produce an ionized first and second element tag, and simultaneously detecting the ionized first and second element tag. The ionization may be performed by an inductively coupled plasma (ICP) torch. The method may further comprise nebulizing the cells in suspension prior to ionizing. In certain embodiments, the ionized first and second element tag may be detected by time-of-flight mass spectrometry. Additional methods, and systems and reagents for simultaneous detection of RNA and protein by mass spectrometry are also described.

    Análisis de expresión génica con oligonucleótidos etiquetados con elementos
    7.
    发明专利
    Análisis de expresión génica con oligonucleótidos etiquetados con elementos 未知

    公开(公告)号：ES2582169T3

    公开(公告)日：2016-09-09

    申请号：ES07710634

    申请日：2007-02-13

    Applicant: FLUIDIGM CANADA INC

    Inventor： ORNATSKY OLGA

    IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/00 ,  C40B30/04 ,  C40B70/00 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

    Abstract: Un método para el análisis celular en una célula o partícula celular, en donde dicha célula es una célula entera de origen animal, vegetal, bacteriano o fúngico, o dicha partícula celular se selecciona del grupo que consiste en un cromosoma aislado, un núcleo aislado, una mitocondria aislada, un cloroplasto aislado, y un virus aislado, comprendiendo el método: (a) fijar y permeabilizar la célula o la partícula celular; (b) incubar la célula o la partícula celular en una solución de hibridación con una sonda de ácido nucleico específica para un ácido nucleico diana, donde la sonda marcada con una etiqueta elemental única tal que un tipo de dicha sonda marcada con un tipo de dicha etiqueta es distinguible de cualquier otro tipo de dicha sonda marcada con un tipo diferente de dicha etiqueta por análisis elemental ICP-MS; (c) separar la sonda no hibridada de la sonda hibridada al ácido nucleico diana mediante estrictas condiciones de lavado; y (d) analizar la célula o partícula celular mediante ICP-MS para identificar la sonda y cuantificar la sonda unida al ácido nucleico diana, en donde cada elemento marcado comprende un resto químico que incluye un átomo elemental o una multitud de átomos elementales con uno o muchos isótopos unidos a una estructura molecular de soporte, y en donde la etiqueta elemental comprende además unos medios de unión de dicha etiqueta a un sustrato.

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