公开(公告)号：WO1982000201A1

公开(公告)日：1982-01-21

申请号：PCT/US1981000899

申请日：1981-06-30

Applicant: BECKMAN INSTRUMENTS INC

Inventor： BECKMAN INSTRUMENTS INC ,  SCHMIDT R ,  PEACOCK J

IPC: G01N01/22

CPC classification number: G01N21/62

Abstract: Un appareil ameliore permettant de melanger un gaz d'echantillon avec un gaz metastable est utilise dans un systeme d'analyse de constituants d'un echantillon par spectroscopie par emission de transfert metastable. L'appareil presente un passage non obstrue pour l'ecoulement du gaz d'echantillon de maniere a reduire au minimum le depot de l'echantillon (10) sur la paroi de l'appareil. L'appareil peut comprendre un collecteur annulaire (14) monte coaxialement autour d'une conduite (32) au travers de laquelle l'echantillon (10) s'ecoule, ou peut comprendre un collier (34) connecte de maniere detachable en ligne avec cette conduite (32).

公开(公告)号：WO1998022509A1

公开(公告)日：1998-05-28

申请号：PCT/US1997020935

申请日：1997-11-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KUUS-REICHEL, Kristine ,  LINTON, Harry, Jay ,  PAYNE, Janice, K. ,  WANG, Tang, Jang

IPC: C07K16/40

CPC classification number: C12N9/6445 ,  C07K14/8121 ,  C07K16/38 ,  C07K16/40

Abstract: Monoclonal antibodies have been developed which specifically bind the PSA-ACT complex without significant cross-reactivity with PSA, ACT or CG-ACT. The antibodies were generated using a unique immunogen comprising a PSA-ACT complex having bound to monoclonal antibodies against PSA and monoclonal antibodies against ACT. It is theorized that the antibodies against PSA and ACT block immunodominant sites on the PSA-ACT complex. The antibodies generated against the unique immunogen specifically bind the PSA-ACT complex, but have no significant cross-reactivity with either PSA or ACT. The monoclonal antibodies of the present invention can be used in immunoassays to specifically detect PSA-ACT in a sample.

Abstract translation: 已经开发了单克隆抗体，其特异性结合PSA-ACT复合物，而与PSA，ACT或CG-ACT没有显着的交叉反应性。 使用独特的免疫原产生抗体，所述免疫原包含结合针对PSA的单克隆抗体和针对ACT的单克隆抗体的PSA-ACT复合物。 理论上，PSA和ACT抗体在PSA-ACT复合物上阻断免疫显性位点。 针对独特免疫原产生的抗体特异性结合PSA-ACT复合物，但与PSA或ACT无明显交叉反应。 本发明的单克隆抗体可用于免疫测定以特异性检测样品中的PSA-ACT。

公开(公告)号：WO1998000701A1

公开(公告)日：1998-01-08

申请号：PCT/US1997006994

申请日：1997-04-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BARBER, Duane, G. ,  TU, Songtai ,  WATTS, Richard, P.

IPC: G01N21/51

CPC classification number: G01N21/532 ,  G01N21/253 ,  G01N35/025 ,  G01N35/1004 ,  G01N2035/00435 ,  G01N2035/00534 ,  G01N2035/00752 ,  G01N2035/0451 ,  G01N2035/1086 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/115831

Abstract: A nephelometer and nephelometer/turbidimeter combination of the type useful in automated chemical analyzers is provided. The combination includes a laser for generating a polarized laser beam having an S-wave component and a P-wave component. The beam is split by a beam splitter specially constructed so that a known proportion of one of the two polarized portions of the beam is directed to a reaction container. In the reaction container, a first polarized component of the laser beam is used in a nephelometric chemical analysis. The remainder of the laser beam passes through the beam splitter to a laser control light detector. Before the remainder of the laser beam reaches the laser control light detector, however, the polarized component which is not used in nephelometric chemical analysis is filtered out. The laser control detector uses the non-filtered portion of the laser beam to control the output of the laser. The combination also includes an LED which emits a beam of light at a longer wavelength than that of the laser. Such beam of light is projected through the reaction container to a turbidimeter light detector. A second beam splitter is provided between the reaction container and the turbidimeter light detector to deflect the residual portion of the laser beam away from the laser. This prevents the residual portion of the laser beam from being reflected back into the reaction container.

Abstract translation: 提供了一种可用于自动化学分析仪的浊度计和浊度计/浊度计组合。 该组合包括用于产生具有S波分量和P波分量的偏振激光束的激光器。 光束被特别构造的分束器分裂，使得梁的两个偏振部分中的一个的已知比例被引导到反应容器。 在反应容器中，激光束的第一偏振分量用于比浊法化学分析。 激光束的其余部分通过分束器通过激光控制光检测器。 然而，在激光束的其余部分到达激光控制光检测器之前，滤除在比浊法化学分析中未使用的偏振分量。 激光控制检测器使用激光束的未滤波部分来控制激光器的输出。 该组合还包括发射比激光器更长波长的光束的LED。 这样的光束通过反应容器投射到浊度计光检测器。 第二分束器设置在反应容器和浊度计光检测器之间，以将激光束的剩余部分偏离激光。 这防止激光束的剩余部分反射回到反应容器中。

公开(公告)号：WO1998000700A1

公开(公告)日：1998-01-08

申请号：PCT/US1997009152

申请日：1997-05-30

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WATTS, Richard, P. ,  MC NEAL, Jack, D.

IPC: G01N21/47

CPC classification number: G01N21/4785 ,  G01N21/278

Abstract: A non-liquid scatter standard for use in nephelometry and turbidimetry systems which comprises a clear silicon rubber gel in which an effective light scattering amount of inorganic particles are suspended. The particles have an index of refraction greater than that of the silicon rubber gel.

Abstract translation: 一种用于比浊法和比浊法系统的非液体散射标准，其包括悬浮有效光散射量的无机颗粒的透明硅橡胶凝胶。 颗粒的折射率大于硅橡胶胶的折射率。

公开(公告)号：WO1997040458A2

公开(公告)日：1997-10-30

申请号：PCT/US1997006648

申请日：1997-04-21

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, M., Parameswara ,  MICHAEL, Maged, A. ,  FAROOQUI, Firdous

IPC: G06F17/30

CPC classification number: C07H21/00 ,  Y02P20/55

Abstract: Universal solid support oligonucleotide synthesis reagents, oligonucleotide synthesis processes, and reagents for cleaving oligonucleotides from solid supports are disclosed. Oligonucleotide synthesis reagents have the following general formula: SS - R - O - R wherein SS is a solid support; R is (a), (b) or (c), where R is hydrogen or alkyl and R is a phosphate protecting group; and R is a ring moiety having vicinal groups -XR and -YR wherein each of X and Y is independently selected from the group consisting of O, S and NH and one of R and R is a blocking moiety and the other is hydrogen or a hydroxy protecting group. Oligonucleotide cleaving reagents include methylamine and/or ammonium hydroxide and trimethylamine.

Abstract translation: 公开了通用固体支持寡核苷酸合成试剂，寡核苷酸合成方法和用于从固体支持物切割寡核苷酸的试剂。 寡核苷酸合成试剂具有以下通式：SS-R 6 -O-R 3其中SS是固体支持物; R 6是（a），（b）或（c），其中R 5是氢或烷基，R 4是磷酸酯保护基; 并且R 3是具有连位-XR 1和-YR 2的环部分，其中X和Y各自独立地选自O，S和NH以及R 1和 R 2是封端部分，另一个是氢或羟基保护基团。 寡核苷酸切割试剂包括甲胺和/或氢氧化铵和三甲胺。

公开(公告)号：WO1997032212A1

公开(公告)日：1997-09-04

申请号：PCT/US1997002748

申请日：1997-02-24

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OBREMSKI, Robert ,  SILZEL, John, W.

IPC: G01N33/543

CPC classification number: G01N21/648 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/54373

Abstract: A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having thereon a plurality of probes of known recognition to the target analytes. The probes are in discrete areas on the waveguide. A sample containing target analyte is treated with a light-responsive compound such that it binds to the target analyte to form a conjugate and the conjugate is applied to the probes on the waveguide. A laser light is passed into the waveguide so that evanescent waves radiate from the waveguide. Where conjugate has attached to probe there is emission of light different from that emitted by a probe without conjugate attached thereto.

Abstract translation: 用于在潜在地含有目标分析物的样品上同时进行多个配体测定的系统用作其上具有已知识别到目标分析物的多个探针的检测器。 探针位于波导上的离散区域中。 用光响应化合物处理含有目标分析物的样品，使其与目标分析物结合形成共轭物，并将缀合物施加到波导上的探针上。 将激光传送到波导中，使得ev逝波从波导辐射。 当缀合物连接到探针上时，发射不同于由其连接的缀合物的探针发射的光。

公开(公告)号：WO1997008545A1

公开(公告)日：1997-03-06

申请号：PCT/US1996013165

申请日：1996-08-12

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PENTONEY, Stephen, L., Jr. ,  PETERSON, Brian, D. ,  OSBORNE, James, C. ,  KEENAN, Charles, A.

IPC: G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44782

Abstract: A gel pump operated by a stepper motor may be used to deliver fresh gel to one or more capillaries through one or more manifolds and valves. By controlling the valves, fresh gel delivered by the pump will replace the old gel in the capillaries for an automated gel replacement system. In a different setting of the valves, the manifolds may be purged of the old gel prior to delivery of fresh gel to the capillaries. The gel delivery system may also be combined with an electrophoresis system so that sealing connection capable of withstanding high pressure adequate for gel injection need not be frequently broken when the gel is to be replaced. Manifold/reflector assembly is advantageously used in the system that facilitates electrical circuit for electrophoresis and for gel replacement.

Abstract translation: 由步进电动机操作的凝胶泵可以用于通过一个或多个歧管和阀将新鲜的凝胶递送到一个或多个毛细管。 通过控制阀门，由泵输送的新鲜凝胶将替代毛细管中的旧凝胶，用于自动凝胶置换系统。 在阀的不同设置中，在将新鲜凝胶递送至毛细管之前，歧管可以被清除旧凝胶。 凝胶递送系统还可以与电泳系统组合，使得当要更换凝胶时，不需要频繁地破坏能够耐受用于凝胶注射的高压的密封连接。 歧管/反射器组件有利地用于便于用于电泳和凝胶置换的电路的系统中。

公开(公告)号：WO1996029571A1

公开(公告)日：1996-09-26

申请号：PCT/US1996003841

申请日：1996-03-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KAYE, Wilbur ,  PENTONEY, Stephen, L., Jr.

IPC: G01B11/27

CPC classification number: G01N27/44782 ,  G01N21/65 ,  G01N27/44704 ,  G01N27/44721 ,  G01N30/74

Abstract: A system and method are disclosed for optically aligning a capillary tube and an excitation laser beam for fluorescence detection applications by utilizing the Raman scatter signals of the capillary tube's contents. For example, Raman scatter by an electrophoretic separation matrix may be used for alignment in a capillary electrophoresis system. Fluorescent material may be present and may also be used for alignment purposes, but is not necessary. The invention employs a parabolic reflector, having apertures through which the capillary tube and the laser beam are guided so that they intersect, preferably at right angles and at the focal point of the reflector. The Raman scatter signals of the material within the capillary tube are collected via a series of filters and this information is used to reposition, if necessary, a focusing lens that directs the excitation beam into the reflector and the capillary tube, so that the Raman scatter signals are maximized. Maximal Raman scatter signals indicate proper alignment of the capillary tube and the excitation beam. Other signals, such as fluorescence emission from the sample, may then be gathered. Adjustment of the focusing lens may be automated so that alignment of the capillary tube and the beam is maintained throughout analysis of the tube's contents. Sequential alignment of an array of capillary tubes with an excitation beam is also disclosed.

Abstract translation: 公开了一种用于通过利用毛细管内容物的拉曼散射信号将毛细管和激发激光束用于荧光检测应用的系统和方法。 例如，通过电泳分离基质的拉曼散射可以用于毛细管电泳系统中的比对。 荧光材料可以存在并且也可以用于对准目的，但不是必需的。 本发明采用抛物面反射器，其具有孔，毛细管和激光束通过该孔具有孔，使得它们优选地以直角相交，并在反射器的焦点处相交。 毛细管内的材料的拉曼散射信号通过一系列滤光片收集，并且该信息用于重新定位（如果需要的话）将激发光束引导到反射器和毛细管中的聚焦透镜，使得拉曼散射 信号最大化。 最大拉曼散射信号表示毛细管和激发光束的正确对准。 然后可以收集其它信号，例如来自样品的荧光发射。 聚焦透镜的调整可以是自动化的，以便在分析管的内容物期间保持毛细管和束的对准。 还公开了具有激发束的毛细管阵列的顺序对准。

公开(公告)号：WO1996006948A1

公开(公告)日：1996-03-07

申请号：PCT/US1995010226

申请日：1995-08-10

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, M., Parameswara ,  STERNBERG, James, C.

IPC: C12Q01/68

CPC classification number: C12Q1/6825 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6834

Abstract: Double stranded nucleic acid duplexes serve as universal harvestable and cleavable link systems in a variety of different types of immnuoassays (e.g., sandwich, competitive, etc.). Depending upon the type of assay, at least one specific component involved in the assay system is attached to a first member of a pair of sequences forming a double stranded nucleic acid (i.e., two oligonucleotides comprising substantially complementary sequences). The assay is carried out in the presence of a support to which is attached an oligonucleotide which is the other member of the pair of sequences forming a double-stranded nucleic acid duplex under hybridization conditions. Upon the hybridization of the two complementary oligonucleotides to form a duplex, the component of the assay system to which the first member of the pair of oligonucleotides is attached may thereby be effectively removed from the solution phase and harvested onto the support. Oligonucleotides bound to a support are reusable in multiple successive assays. Moreover, any given support-bound oligonucleotide can be used in accordance with the present invention for the analysis of a variety of different analytes. In many cases, the assay system includes a label to facilitate quantifying the amount of analyte; in others, the amount of analyte may be determined without the use of any extraneous label.

Abstract translation: 双链核酸双链体在各种不同类型的免疫测定（例如，三明治，竞争性等）中用作通用可收获和可切割的连锁系统。 根据测定的类型，参与测定系统的至少一个特定组分连接到形成双链核酸的一对序列的第一个成员（即，包含基本上互补序列的两个寡核苷酸）。 所述测定在载体的存在下进行，其中连接寡核苷酸，其是在杂交条件下形成双链核酸双链体的一对序列的另一个成员。 当两个互补寡核苷酸杂交以形成双链体时，可以从溶液相中有效地从溶液相中除去与该对寡核苷酸的第一个成员所连接的测定系统的组分并收获到载体上。 结合支持物的寡核苷酸可在多次连续测定中重复使用。 此外，根据本发明，可以使用任何给定的支持结合寡核苷酸来分析各种不同的分析物。 在许多情况下，测定系统包括有助于量化分析物量的标签; 在其他方面，可以在不使用任何外来标签的情况下确定分析物的量。

公开(公告)号：WO1995014029A1

公开(公告)日：1995-05-26

申请号：PCT/US1994013331

申请日：1994-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, M., P. ,  FAROOQUI, Firdous

IPC: C07H19/06

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  Y02P20/55

Abstract: Disclosed herein are protecting groups for exocyclic amino groups of the base cytosine for use in the synthesis of oligonucleotides and oligonucleoside phosphorothioates, the protecting groups being represented by the formula: -CO-(CH2)0-9-CH3. In a particularly preferred embodiment, the base cytosine is protected with acetyl (-CO-CH3), and the oligonucleotide or oligonucleoside phosphorothioate incorporating the protected cytosine is subjected to a cleavage/deprotection reagent comprising methylamine and ammonia.

Abstract translation: 本文公开了用于合成寡核苷酸和寡核苷酸硫代磷酸酯的碱基胞嘧啶的环外氨基的保护基团，保护基由式-CO-（CH 2）0-9 -CH 3表示。 在一个特别优选的实施方案中，用乙酰基（-CO-CH 3）保护碱基胞嘧啶，并将含有保护的胞嘧啶的寡核苷酸或寡核苷硫代磷酸酯进行包含甲胺和氨的切割/去保护试剂。