公开(公告)号：KR1020080037297A

公开(公告)日：2008-04-30

申请号：KR1020060104180

申请日：2006-10-25

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  김민근

IPC: C12N9/24

Abstract: A method for increasing activity of an enzyme for a multi-glycosyl hydrolase single enzyme Cel44C is provided to obtain multi-functional singly enzyme mutant enzymes with significantly increased enzyme activity of cellulase, xylanase, lichenase or mannanase compared to a wild-type Cel44C by removing a C-terminal of the Cel44C molecular-biologically, thereby being very useful for a paper manufacturing industry, and an environment industry and an agricultural industry for producing organic farm. A method for increasing activity of cellulase, xylanase, lichenase, and mannanase of a wild-type Cel44C comprises the steps of: (a) inserting an artificial termination codon into a C-terminal gene sequence of a wild-type multi-functional single enzyme Cel44C(NCBI access no. DQ367923) to construct a primer; (b) performing a polymerase chain reaction using the primer and cloning it into a cloning vector to prepare a mutant; (c) transforming the mutant; (d) investigating the plant cell wall lytic enzyme activity of the transformed mutant; and (e) measuring each enzymatic activity of the transformed mutant and the wild-type Cel44C through a reducing sugar quantitation technique, wherein the primer consists of sequences described in SEQ ID : NOs. 3 to 6.

Abstract translation: 提供了一种增加多糖基水解酶单酶Cel44C酶活性的方法，以获得多功能单酶突变酶，与野生型Cel44C相比，通过除去纤维素酶，木聚糖酶，地衣酶或甘露聚糖酶具有显着增加的酶活性 Cel44C的C-末端分子生物学，因此对造纸工业，环境工业和农业生产有机农场非常有用。 增加野生型Cel44C的纤维素酶，木聚糖酶，地衣酶和甘露聚糖酶活性的方法包括以下步骤：（a）将人工终止密码子插入野生型多功能单酶的C末端基因序列 Cel44C（NCBI通路编号DQ367923）构建引物; （b）使用引物进行聚合酶链反应并将其克隆入克隆载体中以制备突变体; （c）转化突变体; （d）调查转化突变体的植物细胞壁溶解酶活性; 和（e）通过还原糖定量技术测量转化的突变体和野生型Cel44C的每种酶活性，其中引物由SEQ ID NO：中描述的序列组成。 3〜6。

公开(公告)号：KR1020170122684A

公开(公告)日：2017-11-06

申请号：KR1020170054433

申请日：2017-04-27

Applicant: 경상대학교산학협력단

Inventor： 김현준 ,  조계만 ,  리권수

IPC: A23L33/105 ,  A23L5/20 ,  A61K36/72

Abstract: 본발명은특정플라보노이드및 폴리페놀화합물함량이증진된헛개나무추출물의제조방법에관한것으로, 보다상세하게는본 발명의산화발효를포함하는제조방법으로제조된헛개나무추출물은특정플라보노이드및 폴리페놀화합물함량이다량증진되어기능성성분이강화될뿐만아니라, 헛개나무잎의비린내를제거함으로써기능성음료등에유용하게이용될수 있다.

Abstract translation: 本发明中，通过包括本发明的氧化发酵过程中产生更特别heotgae木提取物是特定的类黄酮和多酚化合物涉及一种方法，用于增强特定的类黄酮和多酚含量heotgae叶的制备 不仅功能性成分得到加强，而且枳叶的豆芽也被去除以有效地用于功能性饮料等。

公开(公告)号：KR1020170072545A

公开(公告)日：2017-06-27

申请号：KR1020150180827

申请日：2015-12-17

Applicant: 경상대학교산학협력단

Inventor： 박기훈 ,  조계만 ,  육흥주 ,  송영훈

IPC: A01G7/06 ,  A01G1/00 ,  A23L11/00 ,  A23L1/30 ,  A23F3/34 ,  A61K36/48 ,  A61K8/97 ,  A61Q19/00

Abstract: 본발명은콩잎의하이드록시프롤린(hydroxyproline) 및아르기닌(arginine)의함량을증가시키는방법에관한것으로, 파종후 40일이경과한콩잎에에틸렌을 200 ppm의농도로처리한결과, 처리후 1일부터유리아미노산의함량은현저히증가하여처리후 3 ~ 7일경에최대치가됨을확인하였고, 또한, 에틸렌을처리하지않은콩잎에비해에틸렌을처리한콩잎에서필수아미노산은약 10배및 비필수아미노산은약 7배이상증가하였으며, 특히, 대표적인기능성소재인감마-아미노부티르산(gamma-aminobutylic acid)은약 2배증가하였고, 하이드록시프롤린은약 10배이상및 아르기닌은약 30배이상증가하였으며, 상기콩잎추출물이피부주름생성과관련이있는엘라스테이즈(elastase)의활성을현저하게저해함을확인함으로써, 본발명의에세폰또는에틸렌을콩잎에처리하는방법은콩잎의하이드록시프롤린및 아르기닌의함량을증가시키는방법으로써유용하게사용될수 있고, 또한상기콩잎, 콩잎추출물, 또는이의분획물을포함하는조성물은피부미용소재및 식의약소재로써사용될수 있다.

Abstract translation: 本发明涉及提高大豆叶中羟脯氨酸和精氨酸含量的方法，当播种后40天的大豆叶用乙烯浓度为200ppm处理时， 处理后游离氨基酸含量显着增加至3〜7天左右，其中必需氨基酸含量比非乙烯处理的油菜籽高约10倍 特别地，作为典型功能材料的γ-氨基丁酸增加约2倍，羟脯氨酸增加约10倍，精氨酸增加约30倍或更多。 可以肯定，与皮肤皱纹形成有关的弹性蛋白酶的活性被显着抑制，因此，用大豆叶处理本发明的食道或乙烯的方法是治疗羟脯氨酸和 它可以是作为增加非环绕的含量，组合物还包含kongip的方法是有用的，kongip提取物，或它们的级分可以被用作表层材料，所以药物材料。

公开(公告)号：KR1020080102873A

公开(公告)日：2008-11-26

申请号：KR1020070049884

申请日：2007-05-22

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  김민근

IPC: C12P7/54

CPC classification number: A01G18/00 ,  C07D209/12 ,  C12P17/10

Abstract: A method of separating indole acetic acid from Pseudomonas strain is provided to promote rearing of mushroom, to reduce considerably a cost for producing oil and a labor cost and to be used in order to develop mushroom culture medium for the low cost and to culture other edible mushrooms besides the pine mushroom, gardening and vegetables. An indole acetic acid is separated from Pseudomonas sp. 7014 KACC 91277P strain culture fluid or an ethylacetate layer extract of the culture fluid. A method of stimulating a growth of the edible mushrooms comprises a step of mixing the indole acetic acid and an amino acid at a rate of 1:1(v/v). The ethylacetate layer extract has an activity of stimulating a growth of the Pseudomonas sp. 7014 KACC 91277P strain.

Abstract translation: 提供了从假单胞菌属菌株中分离吲哚乙酸的方法，以促进蘑菇的饲养，大大降低生产油的成本和劳动成本，并用于低成本开发蘑菇培养基并培养其他可食用 蘑菇除松蘑菇，园艺和蔬菜外。 吲哚乙酸与假单胞菌属（Pseudomonas sp。）分离。 7014 KACC 91277P菌株培养液或培养液的乙酸乙酯层提取物。 刺激可食用蘑菇生长的方法包括以1：1（v / v）的速率混合吲哚乙酸和氨基酸的步骤。 乙酸乙酯层提取物具有刺激假单胞菌属（Pseudomonas sp。）生长的活性。 7014 KACC 91277P菌株。

公开(公告)号：KR1020080070127A

公开(公告)日：2008-07-30

申请号：KR1020070007760

申请日：2007-01-25

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  홍수영 ,  조계만

IPC: A23L1/238 ,  A23L1/202 ,  A23L1/28

CPC classification number: A23L27/50 ,  A23L29/065 ,  A23L31/00 ,  C12R1/07

Abstract: A method of rapidly producing fermented soybeans and soy sauce is provided to obtain fermented soybeans and soy sauce with a high content of surfactin exhibiting cytotoxicity against breast cancer cells and intestinal cancer. Bacillus pumilus HY1 strains(KACC 91280P) having excellent surfactin producing capacity are isolated from Korean traditional soy sauce. Surfactin and 5 types of isomers are identified from the Bacillus pumilus HY1 strains. Soy sauce is rapidly prepared by using the Bacillus pumilus HY1 strains as a starter. The soy sauce using Bacillus pumilus HY1 strains as a starter is fermented about 15 days faster than untreated control group. The surfactin content of soy sauce 45 days after fermentation exhibits 0.008mg/L in case the Bacillus pumilus HY1 strains are inoculated, as compared with 0.005mg/L in case the Bacillus pumilus HY1 strains are not inoculated.

Abstract translation: 提供快速生产发酵大豆和酱油的方法，以获得具有高表达活性的含量的发酵大豆和酱油，其对乳腺癌细胞和肠癌具有细胞毒性。 从韩国传统酱油中分离出具有优异表面活性素生产能力的短小芽孢杆菌HY1菌株（KACC 91280P）。 从短小芽孢杆菌HY1菌株鉴定枯草芽孢杆菌素和5种异构体。 通过使用短小芽孢杆菌HY1菌株作为起始物快速制备酱油。 使用短小芽孢杆菌HY1菌株作为起始物的酱油比未处理的对照组发酵约15天。 在接种短小芽孢杆菌HY1菌株的情况下，发酵45天后的酱油的表面活性素含量为0.008mg / L，与未接种短小芽孢杆菌HY1菌株相比，为0.005mg / L。

公开(公告)号：KR100817321B1

公开(公告)日：2008-03-27

申请号：KR1020060101510

申请日：2006-10-18

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만

IPC: C12N15/55 ,  C12N15/10

Abstract: A sequence of an esterase coding gene(est5S) is provided to be isolated from ruminant stomach bacteria, which is not be cultured by a conventional technique. An esterase is provided to be a novel esterase not belonging to a conventional fat hydrolysis group and show significant effect on metal ions and organic solvent, thereby being useful for food industry, environment industry, agricultural industry and medicinal industry. A novel esterase gene hydrolyzing fat includes a sequence described as SEQ ID : NO. 1 derived from ruminant stomach bacteria identified from cow rumen metagenome. An esterase hydrolyzing the fat includes an amino acid sequence described as SEQ ID : NO. 2 derived from the ruminant stomach bacteria identified from cow rumen metagenome.

Abstract translation: 提供酯酶编码基因（est5S）的序列，从反刍动物胃细菌中分离出来，不用常规技术培养。 提供酯酶作为不属于常规脂肪水解基团的新型酯酶，对金属离子和有机溶剂具有显着影响，从而可用于食品工业，环境工业，农业和医药行业。 水解脂肪的新型酯酶基因包含SEQ ID NO： 1来源于反刍动物胃细菌，从牛瘤胃巨噬细胞中鉴定。 水解脂肪的酯酶包括如SEQ ID NO：1所示的氨基酸序列。 2来源于从牛瘤胃巨噬细胞中鉴定的反刍动物胃细菌。

公开(公告)号：KR101825104B1

公开(公告)日：2018-02-02

申请号：KR1020160055214

申请日：2016-05-04

Applicant: 경상대학교산학협력단

Inventor： 김현준 ,  황정은 ,  정순웅 ,  조계만 ,  박상원

IPC: A23L1/30 ,  A23L5/30 ,  A23L1/28 ,  A61K36/074

Abstract: 본발명의영지버섯() 초음파추출물은세포독성이없으므로, 안전성을확보할수 있으며, 통상적인방법으로제조한영지버섯에탄올추출물에대비하여세포내 지질축적을더 효과적으로억제하므로, 지방간질환의건강기능식품또는치료제로유용하게이용될수 있다.

Abstract translation: 根据本发明，本发明的灵芝超声提取物不具有细胞毒性，因此可以确保安全性; 并且与通过常规方法生产的灵芝的乙醇提取物相比，通过灵芝超声提取物可以更有效地抑制细胞内脂质积聚。 因此，灵芝超声提取物可以有效地用作健康功能食品或脂肪肝疾病的治疗剂。

公开(公告)号：KR1020170125497A

公开(公告)日：2017-11-15

申请号：KR1020160055214

申请日：2016-05-04

Applicant: 경상대학교산학협력단

Inventor： 김현준 ,  황정은 ,  정순웅 ,  조계만 ,  박상원

IPC: A23L1/30 ,  A23L5/30 ,  A23L1/28 ,  A61K36/074

CPC classification number: A23L33/10 ,  A23L5/32 ,  A23L31/00 ,  A23V2002/00 ,  A23V2200/3262 ,  A23V2250/208 ,  A23V2300/48 ,  A61K36/074

Abstract: 본발명의영지버섯() 초음파추출물은세포독성이없으므로, 안전성을확보할수 있으며, 통상적인방법으로제조한영지버섯에탄올추출물에대비하여세포내 지질축적을더 효과적으로억제하므로, 지방간질환의건강기능식품또는치료제로유용하게이용될수 있다.

Abstract translation: 由于没有灵芝（）本发明的超声波提取物是细胞毒性的，并且能够确保安全性，因为在以通常的方式细胞制备的灵芝乙醇提取物的情况下抑制脂肪肝疾病的脂质积聚更有效，膳食补充剂 或作为治疗剂。

公开(公告)号：KR1020090132230A

公开(公告)日：2009-12-30

申请号：KR1020080058396

申请日：2008-06-20

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  허재영 ,  이영한 ,  윤명근

IPC: C12N1/20 ,  C12N1/00

Abstract: PURPOSE: A method for producing shell powder fertilizer using Bacillus licheniformis CBFOS-03 is provided to produce environmentally-friendly shell powder and obtain GlcNAc and chitin oligosaccharide which is widely used in medicine, agriculture, cosmetic and food. CONSTITUTION: A Bacillus licheniformis CBFOS-03(deposit number: KACC 91346P) having chitinous substance lysis ability of shell contains amino acid of sequence number 1. A chi18B gene having the shell chitinous lysis ability contains sequence of sequence number 2. The Bacillus licheniformis CBFOS-03 or chi18B gene is used in producing shell powder fertilizer by biologically and physically treating shell.

Abstract translation: 目的：提供使用地衣芽孢杆菌CBFOS-03生产壳粉的方法，生产环保壳粉，获得广泛应用于医药，农业，化妆品，食品等的GlcNAc和几丁质寡糖。 构成：具有壳的壳质裂解能力的地衣芽孢杆菌CBFOS-03（保藏号：KACC 91346P）含有序列号1的氨基酸。具有壳壳层裂解能力的chi18B基因含有序列号2的序列。地衣芽孢杆菌CBFOS -03或chi18B基因用于通过生物和物理处理壳来生产壳粉末肥料。

公开(公告)号：KR100899170B1

公开(公告)日：2009-05-26

申请号：KR1020070070260

申请日：2007-07-12

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  이영한 ,  조계만

IPC: C12N1/20

Abstract: 본 발명은 퇴비화 중간산물 투입에 의한 발효기간 단축 및 고품질 유기질퇴비의 제조방법에 관한 것으로, 로울러 시스템에서 가축 분뇨와 버섯배양 폐톱밥배지로 제조된 퇴비에서 중기 고온 발효단계 이후에 절대적으로 관여하는 바실러스 휴미(
Bacillus

humi ) 유사체 균주를 제공하는 뛰어난 효과가 있다. 또한, 본 발명은 상기 바실러스 휴미(
Bacillus

humi ) 유사체 균주에 의해 발효된 중간산물 퇴비를 다시 퇴비화 처음 공정으로 재접종함으로써 발효기간을 약 4일 정도 단축할 수 있고, 암모니아 가스량을 발효 최종시기에 약 12.0mg/L로 줄일 수 있을 뿐만 아니라 1.7~0.6mm 이하 퇴비 입자의 분포가 무처리구보다 약 4% 정도 많은 고품질 유기질 비료를 생산할 수 있는 뛰어난 효과가 있다. 따라서, 본 발명의 고품질 유기질 비료는 친환경농업의 농자재로서 농업 및 친환경농자재 생산 산업에 사용할 수 있다.
퇴비화, 가축 분뇨, 버섯배양 폐톱밥배지, 유기질퇴비, 로울러 시스템, 메타게놈, 발효