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    감초, 소맥 및 대조의 혼합 생약 추출물을 유효성분으로 함유하는 뇌신경 보호 효과를 나타내는 식품첨가제
    101.
    发明公开
    감초, 소맥 및 대조의 혼합 생약 추출물을 유효성분으로 함유하는 뇌신경 보호 효과를 나타내는 식품첨가제 无效
    转基因食品添加剂组合物显示胚胎神经细胞的保护活性

    公开(公告)号：KR1020070095485A

    公开(公告)日：2007-10-01

    申请号：KR1020050085355

    申请日：2005-09-13

    Applicant: 대구한의대학교산학협력단

    Inventor： 김상찬 ,  변성희 ,  이종록 ,  양재하 ,  서정철 ,  박숙자

    IPC: A23L1/29 ,  A23L1/22 ,  A23P1/04

    CPC classification number: A23L33/105 ,  A23L27/00 ,  A23L33/40

    Abstract: A food additive composition containing a mixed herb medicine extract of licorice root, wheat, and jujube is provided to be used as a food additive by confirming an excellent cerebral nerve protecting activity which shows the shortening of escape latency through the water-maze performance in animal models having cerebral nerve damage derived by beta amyloid. A food additive composition containing a mixed herb medicine extract of licorice root, wheat, and jujube is prepared by mixing licorice root, wheat, and jujube in a dried weight ratio of 1~10:5~50:1~10; settling the herb mixture in distilled water as a solvent at 30~100°C for 0.5~10hr, wherein the volume ratio of the herb mixture and the distilled water is 1:1~30; hydrothermal-extracting it; centrifuging, filtering, and concentrating the extract, wherein the 0.01~90wt% of the extract is included in 100wt% of the composition. In addition, other herb medicine is able to be selected from the group consisting of Euphoria longana, Bambusae Caulis in Liquamen, Astragali radix, Zizyphi spinosi Semen, Amomum villosum Lour, radish, ginseng, pine needles, red ginseng, Alpinia oxyphylla miquel, Polygala tenuifolia Willdenow, or iris. The extracting solvent is one or more solvents selected from the group consisting of water comprising refined water, C1-C4 lower alcohol, or propylene glycol, ethyl acetate, butylene glycol, ether, and chloroform.

    Abstract translation: 提供含有甘草根，小麦和枣混合草本药物提取物的食品添加剂组合物，用于通过确认优异的脑神经保护活性用作食品添加剂，其通过动物的水迷宫表现显示出逃避潜伏期的缩短 具有由β淀粉样蛋白衍生的脑神经损伤的模型。 通过将甘草根，小麦和枣以1〜10：5〜50：1〜10的干重比混合，制备含有甘草根，小麦和枣混合草药提取物的食品添加剂组合物; 将草药混合物在蒸馏水中作为溶剂在30〜100℃下沉降0.5〜10小时，其中草药混合物与蒸馏水的体积比为1：1〜30; 水热提取; 离心，过滤和浓缩提取物，其中提取物的0.01〜90重量％包括在组合物的100重量％中。 另外，其他草药能够选自丽花蕙兰，百草枯黄芪，黄芪，西葫芦精液，山茱萸，萝卜，人参，松针，红参，杏仁仁，Polygala tenuifolia Willdenow或虹膜。 萃取溶剂是选自由精制水，C1-C4低级醇或丙二醇，乙酸乙酯，丁二醇，乙醚和氯仿组成的组中的一种或多种溶剂。

    황정 추출물을 유효성분으로 포함하는 면역증강용약학조성물
    102.
    发明公开
    황정 추출물을 유효성분으로 포함하는 면역증강용약학조성물 有权
    包含多糖提取物的药物组合物A.增加免疫效果的灰分

    公开(公告)号：KR1020070059395A

    公开(公告)日：2007-06-12

    申请号：KR1020050118198

    申请日：2005-12-06

    Applicant: 대구한의대학교산학협력단

    Inventor： 김영호 ,  양재하 ,  강진희 ,  전도연 ,  김수정 ,  한초롱 ,  이지영 ,  김준석

    IPC: A61K36/8969 ,  A61P37/02

    Abstract: A pharmaceutical composition for increasing immune effects comprising the extract of Polygonatum falcatum A. Gray is provided to increase production of antibodies against foreign antigens and improve a humoral immune response by promoting migration efficiency of leucocytes from peripheral blood into second immune organs. The pharmaceutical composition for increasing immune effects comprises the extract or purified fraction of Polygonatum falcatum A. Gray, wherein the extract of Polygonatum falcatum A. Gray is prepared by heating the root of Polygonatum falcatum A. Gray in water, and the purified fraction of Polygonatum falcatum A. Gray is prepared by adding acetone into the water extract of Polygonatum falcatum A. Gray to prepare 50% water and acetone mixed solvent and removing the precipitated matters from the solution so as to prepare the supernatant containing acetone-soluble components; adding acetone into the supernatant to prepare 80% water and acetone mixed solvent and recovering the precipitated matters; and dissolving the precipitated matters in distilled water and re-precipitating the solution with 80% acetone. A health food for increasing immune effects comprises the extract or purified fraction of Polygonatum falcatum A. Gray, wherein the health food is formulated as powder, granule, tablet, capsule or drink.

    Abstract translation: 提供了一种用于增加免疫效果的药物组合物，其中包括提取物黄um提取物A.用于增加针对外来抗原的抗体的产生，并通过促进白细胞从外周血向第二免疫器官的迁移效率来改善体液免疫应答。 用于增加免疫效果的药物组合物包括黄芪提取物或纯化的部分，其中通过加热水中的黄芪根和灰分的纯化部分制备黄芪提取物， 通过将丙酮加入到黄芪的水提取物中制备灰分，制备50％的水和丙酮混合溶剂，并从溶液中除去沉淀的物质，以制备含丙酮可溶组分的上清液; 向上清液中加入丙酮以制备80％的水和丙酮混合溶剂并回收沉淀的物质; 并将沉淀物溶解在蒸馏水中并用80％丙酮重新沉淀溶液。 用于增加免疫效果的保健食品包括茯苓的提取物或纯化部分，其中保健食品配制成粉末，颗粒，片剂，胶囊或饮料。

    파이로시퀀싱법을 이용한 녹용의 종간 유전자 감별 키트
    103.
    发明授权
    파이로시퀀싱법을 이용한 녹용의 종간 유전자 감별 키트 有权
    通过派生方法鉴定遗传物种遗传鉴定的工具包

    公开(公告)号：KR100673071B1

    公开(公告)日：2007-01-22

    申请号：KR1020050096242

    申请日：2005-10-12

    Applicant: 대구한의대학교산학협력단

    Inventor： 서정철 ,  임강현 ,  정주호 ,  신동훈 ,  정승기 ,  양재하 ,  김상찬

    IPC: C12Q1/68

    CPC classification number: C12Q1/6827 ,  C12Q1/6809 ,  C12Q1/6844

    Abstract: A method for discriminating genetic identification of Cervus species is provided to supply accurate Cervus species raw material by evaluating relation among species and genetic variation of the Cervus species using a species specific sequencing primer of the Cervus species. The method comprises the steps of: (a) extracting DNA from Cervus sample; (b) preparing a reaction solution of primer consisting of the extracted DNA, sense primer(CCCTAGATCA CGAGCTTAAT) and anti-sense primer(TGTCTGATAA AATTCATTAAATAGC); (c) amplifying the reaction solution through PCR; (d) analyzing the total nucleotide sequence of the amplified DNA; and (e) comparing a gene obtained by performing a pyrosequencing technique on the amplified DNA in combination with sequences: CGATTCATGGG, Cervus nippon Temminck, sequences: CAGTTCATGGG, Cervus elaphus from Newzealand, sequences: CGGTTTATGGG, Cervus canadensis, sequences: CAGTATATGGG, Rangifer tarandus so as to discriminate the Cervus nippon Temminck, Cervus elaphus, Cervus canadensis and Rangifer tarandus and comparing the gene with sequences: CGAGTTCATGGG, Cervus elaphus from Russia, and sequences: CGATCTATGGG, Cervus elaphus from China so as to discriminate the Cervus elaphus from Russia and the Cervus elaphus from China.

    Abstract translation: 提供了一种通过使用鹿类物种特异性测序引物评估鹿茸种类与遗传变异关系，提供精确的鹿茸种类原料的方法。 该方法包括以下步骤：（a）从鹿茸样品中提取DNA; （b）制备由提取的DNA，有义引物（CCCTAGATCA CGAGCTTAAT）和反义引物（TGTCTGATAA AATTCATTAAATAGC）组成的引物的反应溶液; （c）通过PCR扩增反应溶液; （d）分析扩增的DNA的总核苷酸序列; 和（e）将通过对扩增的DNA进行焦磷酸测序技术获得的基因与序列组合：CGATTCATGGG，鹿茸Nippon Temminck，序列：CAGTTCATGGG，来自Newzealand的鹿茸，序列：CGGTTTATGGG，加拿大鹿，序列：CAGTATATGGG，Rangifer tarandus 从而区分日本鹿茸Temminck，鹿茸，加拿大鹿茸和Rangifer tarandus，并将该基因与来自俄罗斯的CGAGTTCATGGG，鹿茸和序列：CGATCTATGGG，中国的鹿茸进行比较，从而将鹿茸从俄罗斯和 来自中国的鹿鹿elaphus。

    인삼의 종간 유전자 감별 키트
    105.
    发明授权
    인삼의 종간 유전자 감별 키트 有权
    用于区分人参物种遗传鉴定的试剂盒

    公开(公告)号：KR100610312B1

    公开(公告)日：2006-08-10

    申请号：KR1020040063776

    申请日：2004-08-13

    Applicant: 대구한의대학교산학협력단

    Inventor： 서정철 ,  임강현 ,  정주호 ,  신동훈 ,  최강덕 ,  양재하 ,  김상찬 ,  정승기 ,  한상원

    IPC: C12Q1/68

    Abstract: 본 발명은 파이로시퀀싱(Pyrosequencing)법에 의한 인삼의 종간 유전자를 감별하는 감별 키트에 관한 것으로써, 상세하게는 고려삼과 서양삼을 파이로시퀀싱법에 의하여 유전자형을 분석하고, 인삼의 종간 특이성 시퀀싱 프라이머를 고안하여 파낙스 종(Panax species)의 종간 연관성과 유전적 변이를 평가하기 위한 감별 키트에 관한 것이다. 본 발명의 감별 키트를 통하여 인삼의 종을 판별함으로써 정확한 인삼 원료를 공급할 수 있으며, 인삼의 품질관리 방법으로 활용 할 수 있다.
    고려삼, 서양삼, 파이로시퀀싱(Pyrosequencing)법, 감별 키트

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