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公开(公告)号：KR101034547B1
公开(公告)日：2011-05-12
申请号：KR1020080080141
申请日：2008-08-14
Abstract: 50-125MPa의 압력, 40-60℃의 온도, 12-24 시간 및 0.1-0.5중량%의 효소첨가량의 조건에서 초고압기술을 처리하여 쇠고기 분말을 제조하는 방법 및 상기 제조방법에 의한 쇠고기 분말을 제공한다. 또한, 본 발명은 쇠고기를 분쇄하는 단계; 분쇄 쇠고기를 5-20중량%로 물에 분산시키는 단계; 0.1-0.5중량%의 효소를 첨가하는 단계; 6.0-8.0의 pH로 조절하는 단계; 50-125MPa로 압력처리 하는 단계; 쇠고기 슬러리를 원심분리하여 가수분해물을 얻는 단계; 여과단계; 95-100℃의 온도에서 끓임으로써 효소를 불활성화시키는 단계; 및 쇠고기 조미소재를 수득하는 단계로 이루어진다.
초고압, 온도, 시간, 효소첨가, 조미소재, 쇠고기-
公开(公告)号：KR1020100021293A
公开(公告)日：2010-02-24
申请号：KR1020080080141
申请日：2008-08-14
CPC classification number: A23L27/10 , A22C17/0006
Abstract: PURPOSE: A seasoning material as food additives and a manufacturing method thereof are provided to hinder activity of harmful bacteria with ultra-high pressure technology, and to activate enzymatic reaction. CONSTITUTION: A manufacturing method of a seasoning material as food additives includes the following steps: pulverizing beef; scattering the pulverized beef in water of 5-20 weight%; adding enzyme of 0.1-0.5 weight%; adjusting ph of the material to 6.0-8.0; processing the material in a condition of 40-60°C and pressure of 50-125MPa; gaining hydrolysate by centrifugal of beef slurry; filtering the acquired hydrolysate; inactivating enzyme in a temperature of 95-100°C; and acquiring solid contents.
Abstract translation: 目的：提供作为食品添加剂的调味料及其制造方法，以超高压技术阻碍有害细菌的活性，并活化酶反应。 构成：作为食品添加剂的调味料的制造方法包括以下步骤：粉碎牛肉; 将粉碎的牛肉在5-20重量％的水中分散; 加入0.1-0.5重量％的酶; 调整ph值至6.0-8.0; 在40-60℃的条件下加工材料，压力为50-125MPa; 通过离心牛肉浆获得水解产物; 过滤所获得的水解产物; 在95-100℃的温度下灭活酶; 并获得固体含量。
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公开(公告)号：KR1020100008944A
公开(公告)日：2010-01-27
申请号：KR1020080069583
申请日：2008-07-17
Applicant: 한국식품연구원
Abstract: PURPOSE: A method for measuring C-reactive protein using gold nanoparticle is provided to measure ultra highly-sensitive biomarker in a real time at 100 fM level. CONSTITUTION: A ultra highly-sensitive detection of C-reactive protein comprises: a step of adding reaction buffer to a sensor chip to obtain the number of vibration(F1) at a normal status; a step of adding mixture of streptavidin-coated gold nanoparticle and test sample containing biotinylated C-reactive protein antibody and C-reactive protein on the sensor chip; a step of amplifying signal by binding streptavidin-coated gold nanoparticle to the biotinylated C-reactive protein antibody conjugated with C-reactive protein; and a step of measuring variation of the number of vibration(ΔF = F1 - F2).
Abstract translation: 目的：提供使用金纳米颗粒测量C-反应蛋白的方法，以100 fM水平实时测量超高灵敏度生物标志物。 构成：C-反应蛋白超高灵敏度检测方法包括：将反应缓冲液加入到传感器芯片中以获得正常状态下的振动次数（F1）的步骤; 在传感器芯片上加入链霉抗生物素蛋白包被金纳米颗粒和含有生物素化C反应蛋白抗体和C-反应蛋白的测试样品的混合物的步骤; 通过将链霉抗生物素蛋白包被的金纳米颗粒结合到与C反应蛋白缀合的生物素化的C反应蛋白抗体上来扩增信号的步骤; 以及测量振动次数的变化的步骤（ΔF= F1-F2）。
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公开(公告)号：KR1020090122617A
公开(公告)日：2009-12-01
申请号：KR1020080048520
申请日：2008-05-26
Applicant: 한국식품연구원
CPC classification number: A23L27/40 , A23L27/105 , A23L29/231 , A23L29/238 , A23L29/284
Abstract: PURPOSE: A nanoemulsion, and its manufacturing method are provided to allow the amount of intake of salt to be controlled more easily and to maximize the body's absorption of functional components. CONSTITUTION: A nanoemulsion comprises 0.03~0.3 wt% of a vegetable essential oil; 0.09~1.5 wt% of an emulsifier; 0.1~5.0 wt% of a water-soluble biopolymer; 1.0~30.0 wt% of salt; and the balance of water. Preferably the vegetable essential oil is at least one selected from the group consisting of a hot pepper essential oil, a garlic essential oil, a ginger essential oil and an onion essential oil. The water-soluble biopolymer comprises at least one selected from the group consisting of chitosan, alginic acid, cellulose, carrageenan, gelatin, arabic gum, pectin and guar gum.
Abstract translation: 目的：提供纳米乳液及其制造方法，以便更容易地控制盐的摄入量，并使身体对功能组分的吸收最大化。 构成：纳米乳液含有0.03〜0.3重量％的植物精油; 0.09〜1.5重量％的乳化剂; 0.1〜5.0重量％的水溶性生物聚合物; 1.0〜30.0重量％的盐; 和水的平衡。 优选地，植物精油是选自辣椒精油，大蒜精油，姜精油和洋葱精油中的至少一种。 水溶性生物聚合物包括选自壳聚糖，藻酸，纤维素，角叉菜胶，明胶，阿拉伯树胶，果胶和瓜尔胶中的至少一种。
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105.发明公开Ｃ-반응성 단백 측정용 수정진동자 면역센서 측정장치 및수정진동자 면역센서를 이용한 Ｃ-반응성 단백의 고감도측정방법 有权用于C反应蛋白检测的QUARTZ晶体微生物免疫传感器装置和使用QUARTZ晶体微生物免疫荧光剂的C反应蛋白的高灵敏度检测方法
公开(公告)号：KR1020090073669A
公开(公告)日：2009-07-03
申请号：KR1020070141680
申请日：2007-12-31
Applicant: 한국식품연구원
Abstract: A crystal oscillator immunosensor measurement apparatus for the measurement of a C-reactive protein, and its method are provided to allow C-reactive protein to be measured in real time within a short time by sing a biomarker. A crystal oscillator immunosensor measurement apparatus for the measurement of a C-reactive protein comprises a reaction buffer solution tub; a supply unit which is connected to the reaction buffer solution tub, and supplies the reaction buffer solution of the reaction buffer solution tub to the C-reactive protein fixed to the crystal oscillator sensor chip of a reactive cell; a sample inlet hole which is connected to the supply unit; a reaction cell which comprises a crystal oscillator, an O-ring, and a holder; an oscillation module which oscillates the crystal oscillator inside the reaction cell and is connected to the crystal oscillator to transmit the vibration of the crystal oscillator to a vibration sensor; a vibration sensor which measures the vibration of the crystal oscillator of the reaction by the oscillation module; a computer which is connected to the vibration sensor to show the frequency variation of the C-reactive protein fixed to the sensor chip electrode of the crystal oscillator; and a waste liquid tank.
Abstract translation: 提供用于测量C-反应蛋白的晶体振荡器免疫传感器测量装置及其方法，以通过生物标记物通过生物标记物在短时间内实时测量C反应蛋白。 用于测量C反应蛋白的晶体振荡器免疫传感器测量装置包括反应缓冲溶液槽; 供给单元，其连接到反应缓冲溶液槽，并将反应缓冲溶液槽的反应缓冲溶液供应到固定在反应池的晶体振荡器传感器芯片上的C-反应蛋白; 连接到供应单元的样品入口孔; 包括晶体振荡器，O形环和保持器的反应单元; 振荡模块，其使晶体振荡器在反应单元内部振荡并连接到晶体振荡器，以将振荡器的振动传递到振动传感器; 振动传感器，其测量所述振荡模块对所述晶体振荡器的振动的反应; 连接到所述振动传感器以显示固定到所述晶体振荡器的所述传感器芯片电极的C反应蛋白的频率变化的计算机; 和废液罐。
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Patent Agency Ranking
公开(公告)号：KR1020090057835A
公开(公告)日：2009-06-08
申请号：KR1020070124593
申请日：2007-12-03
Applicant: 한국식품연구원
IPC: C07K19/00
Abstract: A recombinant fusion protein is provided to simply fix all kinds of antibodies and give proper orientation to fixed antibody. A recombinant fusion protein is encoded by sequence number 10 or 11. A polynucleotide has a sequence of sequence number 10 or 11. A biosensor comprises the recombinant fusion protein and gold-containing solid phase. The gold-containing solid phase is selected from a gold colloidal particle, nano-gold particle, and substrate containing gold-coating particle. A method of immuno detection of target material comprises: a step of combining an antibody with the recombinant fusion protein of the biosensor; and a step of treating the antibody-specific antigen to detect the specific binding of antigen-antobody.
Abstract translation: 提供重组融合蛋白以简单地固定所有种类的抗体并给予固定抗体正确取向。 重组融合蛋白由序列号10或11编码。多核苷酸具有序列号10或11的序列。生物传感器包含重组融合蛋白和含金固相。 含金固相选自金胶体颗粒，纳米金颗粒和含有金涂层颗粒的底物。 靶物质的免疫检测方法包括：将抗体与生物传感器的重组融合蛋白结合的工序; 以及治疗抗体特异性抗原以检测抗原 - 附着物的特异性结合的步骤。
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公开(公告)号：KR1020090014947A
公开(公告)日：2009-02-11
申请号：KR1020080044128
申请日：2008-05-13
Applicant: 한국식품연구원
CPC classification number: A23L33/105 , A23L27/10 , A23L29/10 , A23L29/275 , A23V2002/00 , A23V2200/25 , A23V2200/222 , A23V2250/511
Abstract: A nanoemulsion containing a plant essential oil, a method for preparing the nanoemulsion, a nanoparticle prepared from the nanoemulsion, and a method for preparing the nanoparticle are provided to improve the stabilization and the absorption into the human body of a functional components. A nanoemulsion containing a plant essential oil comprises 0.03-0-.3 wt% of a plant essential oil; 0.09-1.5 wt% of sorbitan fatty acid ester; 0.05-1.0 wt% of a water-soluble biopolymer; and the balance of water. Preferably the plant essential oil is at least one selected from a hoe pepper essential oil, a garlic essential oil, a ginger essential oil and an onion essential oil.
Abstract translation: 提供含有植物精油的纳米乳剂，制备纳米乳液的方法，由纳米乳液制备的纳米颗粒，以及制备纳米颗粒的方法，以改善对功能组分的人体的稳定和吸收。 含有植物精油的纳米乳液含有0.03-0.3重量％的植物精油; 0.09-1.5重量％脱水山梨糖醇脂肪酸酯; 0.05-1.0重量％的水溶性生物聚合物; 和水的平衡。 优选地，植物精油是选自锄头精油，大蒜精油，姜精油和洋葱精油中的至少一种。
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公开(公告)号：KR100849156B1
公开(公告)日：2008-07-30
申请号：KR1020070006787
申请日：2007-01-23
Applicant: 한국식품연구원 , 주식회사 에스에프씨바이오
Abstract: 본 발명은 (a) 수박 시료를 야자유 지방산 에틸에스터를 용제로하여 lycopene을 추출회수 하거나, 야자유 지방산 에틸에스터를 인트레인너(entrainer)로 하여, 6,000 psi 이상 및 40~70℃의 조건에서 초임계 이산화탄소를 이용하여 라이코펜을 1차 추출하는 단계, (b) 상기 (a)단계의 라이코펜 추출물을 1600 psi의 압력과 50℃의 온도 조건에서 초임계 이산화탄소를 이용하거나, 진공증류공정을 이용하여 야자유 지방산 에틸에스터를 제거하여 라이코펜이 농축된 추출물을 제조하는 방법에 관한 것으로, 열이나 빛 등의 안정성을 꾀하는 환경 요소를 배제함으로써 효능적으로 안정되며 고수율 및 고농도의 수박 유래 라이코펜인 천연 항산화제를 얻을 수 있다.
라이코펜, 수박, 초임계 이산화탄소, 추출, 안정성-
公开(公告)号：KR1020080069284A
公开(公告)日：2008-07-28
申请号：KR1020070006787
申请日：2007-01-23
Applicant: 한국식품연구원 , 주식회사 에스에프씨바이오
Abstract: A method for preparing a lycopene concentrated extract is provided to isolate lycopene without destruction from watermelon with high concentration, high yield and stability using supercritical CO2, thereby the recovered lycopene being utilized into various kinds of food products. A method for preparing a lycopene concentrated extract comprises the steps of: (a) after crushing watermelon flesh using a chopper, a colloid mill and a homogenizer in sequence, centrifuging the crushed flesh and storing cake and serum, separately; and (b) preparing the lycopene concentrated extract from lipophilized watermelon cake or a wet-state watermelon cake using supercritical CO2. Another method for preparing a lycopene concentrated extract comprises the steps of: (a) extracting lycopene from a wet-stage watermelon sample using a fatty acid ethyl ester of coconut oil as an entraciner and using supercritical CO2; and (b) preparing the lycopene concentrated extract from the lycopene extract obtained from the step(a) under the pressure of 1,600 psi and the temperature of 50 deg.C.
Abstract translation: 提供番茄红素浓缩提取物的制备方法，使用超临界CO2从高浓度，高产率和稳定性的西瓜中分离番茄红素，从而将回收的番茄红素用于各种食品中。 制备番茄红素浓缩提取物的方法包括以下步骤：（a）使用切碎机，胶体磨和匀浆器依次粉碎西瓜肉，分离离心碎肉并分别储存蛋糕和血清; 和（b）使用超临界CO 2从脂肪化西瓜饼或湿态西瓜饼制备番茄红素浓缩提取物。 制备番茄红素浓缩提取物的另一种方法包括以下步骤：（a）使用椰子油的脂肪酸乙酯作为夹带器并使用超临界CO 2从湿阶段西瓜样品中提取番茄红素; 和（b）在1600psi的压力和50℃的温度下，从步骤（a）获得的番茄红素提取物制备番茄红素浓缩提取物。
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公开(公告)号：KR100718165B1
公开(公告)日：2007-05-15
申请号：KR1020050046413
申请日：2005-05-31
Applicant: 한국식품연구원
IPC: A23G3/34
Abstract: 본 발명은 찹쌀전분을 물에 혼합하는 단계; 및 상기 혼합물에 소정의 압력을 가하여 전분입자에 물리적인 충격을 가하여 입자의 크기를 감소시키는 단계를 포함하는 유과제조용 전분의 제조방법을 제공한다. 상기 공정에 의하면 현행 유과제조공정에 있어서 침지공정을 거칠 필요가 없고, 뿐만 아니라 제조공정의 단순화 및 청결성이 강화된 유과제조가 가능하다.
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