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    BACTERIAL HOST STRAIN COMPRISING A MUTANT SPR GENE AND HAVING REDUCED TSP ACTIVITY
    101.
    发明专利
    BACTERIAL HOST STRAIN COMPRISING A MUTANT SPR GENE AND HAVING REDUCED TSP ACTIVITY 未知

    公开(公告)号：SG182301A1

    公开(公告)日：2012-08-30

    申请号：SG2012048567

    申请日：2011-01-13

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    Abstract: The present invention provides a recombinant gram-negative bacterial cell comprising a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, 170, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and wherein the cell has reduced Tsp protein activity compared to a wild-type cell.

    FRAGMENTOS DE ANTICUERPO MODIFICADOS.
    103.
    发明专利
    FRAGMENTOS DE ANTICUERPO MODIFICADOS. 未知

    公开(公告)号：ES2374222T3

    公开(公告)日：2012-02-14

    申请号：ES06764991

    申请日：2006-07-17

    Applicant: UCB PHARMA SA

    Inventor： HUMPHREYS DAVID PAUL

    IPC: C07K16/00 ,  A61K47/48

    Abstract: Fragmento de anticuerpo de IgG al que se une una o más moléculas efectoras seleccionadas de un polímero con un peso molecular promedio en el intervalo de 500 Da a 100.000 Da caracterizado porque está ausente el enlace disulfuro entre cadenas nativas entre las regiones constantes de la cadena pesada (CH1) y ligera (CL) y las regiones constantes de la cadena pesada (CH1) y la cadena ligera (CL) están unidas mediante un enlace disulfuro entre cadenas entre un par de cisteínas, modificadas por ingeniería, una en la región constante de la cadena ligera (CL) y la otra en la región constante de la cadena pesada (CH1), en el que el último enlace disulfuro está ampliamente inaccesible para agentes reductores y moléculas efectoras, de tal manera que permanece intacto durante la unión a molécula efectora y en el que la posición del par de cisteínas, modificadas por ingeniería, se selecciona de la posición 116 de la cadena ligera y 138 de la cadena pesada, la posición 119 de la cadena ligera y 128 de la cadena pesada o la posición 210 de la cadena ligera y 129 de la cadena pesada (numeración de Kabat).

    BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES
    104.
    发明专利
    BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES 未知

    公开(公告)号：CA2774692A1

    公开(公告)日：2011-03-31

    申请号：CA2774692

    申请日：2010-09-23

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    IPC: C12N9/50 ,  C12P21/00

    Abstract: A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.

    BACTERIAL HOST STRAIN EXPRESSING RECOMBINANT DSBC
    107.
    发明专利
    BACTERIAL HOST STRAIN EXPRESSING RECOMBINANT DSBC 未知

    公开(公告)号：CA2841824C

    公开(公告)日：2021-07-13

    申请号：CA2841824

    申请日：2012-07-13

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    IPC: C07K16/28 ,  C12N15/70

    Abstract: The present invention relates to the development of a bacterial periplasmic system for expressing antibodies. Specifically there is provided a recombinant gram-negative bacterial cell comprising: a mutated Tsp gene which is a knockout mutated Tsp gene or which encodes a Tsp protein having reduced protease activity compared to a wild type Tsp gene, a mutated spr gene encoding an spr protein capable of suppressing the growth phenotype of a cell comprising a mutated Tsp phenotype, an expression vector comprising a recombinant polynucleotide encoding DsbC; and one or more polynucleotides encoding an antibody or an antigen binding fragment thereof specifically binding to CD154.

    МОДИФИЦИРОВАННЫЕ БИСПЕЦИФИЧЕСКИЕ АНТИТЕЛА IgG4 С АСИММЕТРИЧНОЙ ПОСЛЕДОВАТЕЛЬНОСТЬЮ
    108.
    发明专利
    МОДИФИЦИРОВАННЫЕ БИСПЕЦИФИЧЕСКИЕ АНТИТЕЛА IgG4 С АСИММЕТРИЧНОЙ ПОСЛЕДОВАТЕЛЬНОСТЬЮ 未知

    公开(公告)号：EA033720B1

    公开(公告)日：2019-11-20

    申请号：EA201491571

    申请日：2013-02-22

    Applicant: UCB PHARMA SA

    Inventor： HUMPHREYS DAVID PAUL ,  PETERS SHIRLEY JANE

    IPC: C07K16/00

    Abstract: Изобретениеотноситсяк асимметричномусмешанномуантителу, содержащемудветяжелыецепиилифрагменттяжелойцепи, каждаяизкоторыхсодержитпоменьшеймеревариабельнуюобласть, шарнирнуюобластьи C1-домен, гдеперваятяжелаяцепьилиеефрагментпринадлежитклассу IgG4 иимеет a) цистеинмежцепочечнойсвязив положении 127 принумерациипосистеменумерации Kabat вдомене C1, замененныйнадругуюаминокислоту; и b) необязательнооднуилинесколькоизаминокислот, расположенныхв верхнейшарнирнойобластии замененныхцистеином, игдевтораятяжелаяцепьилиеефрагментотличаетсятем, чточастьиливсяцепьотличаетсяпоаминокислотнойпоследовательностиотуказаннойпервойтяжелойцепипоменьшеймерев областивневариабельнойобласти (например, вконстантнойобласти), ксоставам, содержащимих, терапевтическомусредству, вкоторомиспользуютсяобаизуказанныхвыше, ик способамполученияантители состава.

    BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES
    109.
    发明专利
    BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES 未知

    公开(公告)号：CA2774692C

    公开(公告)日：2017-12-19

    申请号：CA2774692

    申请日：2010-09-23

    Applicant: UCB PHARMA SA

    Inventor： ELLIS MARK ,  HUMPHREYS DAVID PAUL

    IPC: C12N9/50 ,  C12P21/00

    Abstract: A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.

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