Determination of analytes in a sample matrix by solvent extraction

    公开(公告)号:US10545076B2

    公开(公告)日:2020-01-28

    申请号:US15515794

    申请日:2014-10-20

    Abstract: Determination of Analytes in a Sample Matrix by Solvent Extraction A method for the assay of one or more analytes in a sample matrix comprising the steps of: performing analyte extraction on the sample matrix, said analyte extraction comprising combining the sample matrix with a solvent for an extraction period which is less than that required for reaching equilibrium; and separating the analyte containing solvent from the sample matrix; next measuring a level of analyte present in the separated solvent; and then applying in a computer a calibration by which is established a mathematical relationship between levels of analyte extracted from each of a plurality of reference samples by means of the process employed above in the extraction for the sample matrix and a reference value of the levels of analyte for each reference sample to thereby derive a measure of the level of analyte in the sample matrix. Specifically a method to determine the amount of mycotoxins in cereal grain, especially OTA (ochratoxin A) and DON (deoxynivalenol) by mixing with a solvent comprising water alcohol mixture, with 20-40% ethanol by volume.

    Standardizing the output of an electrical property sensor

    公开(公告)号:US12055506B2

    公开(公告)日:2024-08-06

    申请号:US17632043

    申请日:2020-05-28

    CPC classification number: G01N27/06 G01N21/3577 G01N33/04

    Abstract: A method of standardizing an output of an electrical property sensor includes calculating a reference magnitude (Condr) of an electrical property of an electrically conductive liquid, based on obtaining, by a spectrometer, spectral data resulting from an interaction of light with the electrically conductive liquid, and applying, in a data processor, a mathematical model to the spectral data to calculate the reference magnitude (Condr) of the electrical property, wherein the mathematical model links the spectral data to magnitudes of the electrical property; comparing, in the data processor, a measured magnitude (Condm) of the electrical property of the electrically conductive liquid with a reference magnitude (Condr) of the electrical property, wherein the measured magnitude (Condm) of the electrical property is obtained with the electrical property sensor; determining, in the data processor, a standardization factor (F) dependent on a ratio of the reference magnitude (Condr) and the measured magnitude (Condm).

    Method of correcting for an amplitude change in a spectrometer

    公开(公告)号:US11965823B2

    公开(公告)日:2024-04-23

    申请号:US17629532

    申请日:2020-05-29

    Abstract: A method of correcting for an amplitude change in a spectrometric instrument output includes: exposing a sample in a sample holder to electromagnetic radiation at a plurality of wavenumbers; detecting electromagnetic absorption intensities in the sample at the plurality of wavenumbers; providing to a computer device the detected electromagnetic absorption intensities indexed against wavenumber as spectral data; applying in the computer device a mathematical transform (Icorr) to the spectral data to correct for an amplitude change in the spectrometric instrument's output and calculated by determining a difference (Δ(SBZ)/) between first derivatives of a logarithmic transformation of spectral data (SBZ) from the zero material sample at two different wavenumber ranges (log10(SBZ(x1))/ and log10(SBZ(x2))/); and calculating the mathematical transform (Icorr) as a function inversely dependent on the determined difference (Δ(SBZ)/).

    METHOD OF DETERMINING AN OPTICAL PATHLENGTH THROUGH A CUVETTE

    公开(公告)号:US20230011975A1

    公开(公告)日:2023-01-12

    申请号:US17784870

    申请日:2020-10-15

    Abstract: A method for determining an optical pathlength through a cuvette of a spectrophotometric apparatus includes obtaining a first single beam spectrum of a liquid zero-material at least in a first energy region in which the liquid zero-material absorbs at least a portion of incident optical radiation; obtaining a second single beam spectrum of a second liquid at least in the first energy region, the second liquid having a composition excluding the liquid zero-material and having no absorption of incident optical radiation in the first energy region; determining a dual beam spectrum of the liquid zero-material relative to the second liquid at least in the first energy region from the first and second single beam spectra; and calculating an optical pathlength through the cuvette based on processing spectral information obtained from the first energy region of the determined dual beam spectrum.

    METHOD OF CORRECTING FOR AN AMPLITUDE CHANGE IN A SPECTROMETER

    公开(公告)号:US20220252505A1

    公开(公告)日:2022-08-11

    申请号:US17629532

    申请日:2020-05-29

    Abstract: A method of correcting for an amplitude change in a spectrometric instrument output includes: exposing a sample in a sample holder to electromagnetic radiation at a plurality of wavenumbers; detecting electromagnetic absorption intensities in the sample at the plurality of wavenumbers; providing to a computer device the detected electromagnetic absorption intensities indexed against wavenumber as spectral data; applying in the computer device a mathematical transform (Icorr) to the spectral data to correct for an amplitude change in the spectrometric instrument's output and calculated by determining a difference (Δ(SBZ)/) between first derivatives of a logarithmic transformation of spectral data (SBZ) from the zero material sample at two different wavenumber ranges (log10(SBZ(x1))/ and log10(SBZ(x2))/); and calculating the mathematical transform (Icorr) as a function inversely dependent on the determined difference (Δ(SBZ)/).

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