公开(公告)号：AU2015289999B2

公开(公告)日：2020-09-10

申请号：AU2015289999

申请日：2015-07-10

Applicant: HARVARD COLLEGE

Inventor： HINOJOSA CHRISTOPHER DAVID ,  SLIZ JOSIAH ,  THOMPSON GUY ,  GEISLER HUBERT ,  FERNANDEZ-ALCON JOSE ,  INGBER DONALD E ,  LEVNER DANIEL

IPC: B82Y15/00 ,  C12M1/00 ,  C12Q1/02

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

公开(公告)号：CA2828110C

公开(公告)日：2020-03-31

申请号：CA2828110

申请日：2012-02-28

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  KIM HYUN JUNG

IPC: C12M3/02 ,  C12N1/14 ,  C12N1/20 ,  C12N5/071

Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

公开(公告)号：AU2019268194B2

公开(公告)日：2020-02-20

申请号：AU2019268194

申请日：2019-11-22

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  KIM HYUN JUNG

IPC: C12M3/02 ,  C12N1/14 ,  C12N1/20 ,  C12N5/071

Abstract: Abstract The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora. - 61- WO 2012/118799 PCT/US2012/026934 - -% 0 -- - 2 'CYD C3 (nuzjIEL; - C= SUSIUTSET(UE6

公开(公告)号：AU2018204114B2

公开(公告)日：2019-05-09

申请号：AU2018204114

申请日：2018-06-08

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  SUPER MICHAEL ,  WATTERS ALEXANDER L ,  SNELL PHILIP T

IPC: C07K14/805 ,  A61K38/00

Abstract: Described herein are heme-binding compositions and methods relating to their use, e.g. methods of treatment of, for example, sepsis and rhabdomyolysis.

公开(公告)号：HK1250055A1

公开(公告)日：2018-11-23

申请号：HK18109529

申请日：2018-07-23

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  WEITZ DAVID A ,  DIDIER JONATHAN ,  ZIERINGER MAXIMILIAN

IPC: E21B20060101

公开(公告)号：CA3053191A1

公开(公告)日：2018-08-30

申请号：CA3053191

申请日：2018-02-26

Applicant: PRESIDENT AND FELLLOWS OF HARVARD COLLEGE

Inventor： MAOZ BEN M ,  HERLAND ANNA ,  HENRY OLIVIER F ,  PARKER KEVIN K ,  INGBER DONALD E

IPC: B01L3/00 ,  C12M3/04 ,  C12N5/07 ,  G01N27/40

Abstract: An organ-on-chip device for monitoring a biological function and including a membrane layer located at an interface region between a top microchannel and the a microchannel. The membrane includes a first type of cells forming a barrier between the top microchannel and the bottom microchannel. The device further includes a top layer having a first plurality of transendothelial electrical resistance (TEER) measurement electrodes for enabling direct monitoring of cell function and electrical activity of the first type of cells on the membrane. The device also has a multi-electrode array (MEA) layer with a second plurality of TEER measurement electrodes for enabling direct monitoring of cell function and electrical activity of a second type of cells on the MEA layer.

公开(公告)号：AU2016285700A2

公开(公告)日：2018-01-04

申请号：AU2016285700

申请日：2016-04-25

Applicant: HARVARD COLLEGE

Inventor： INGBER DONALD E ,  VAN DER MEER ANDRIES ,  HERLAND ANNA

IPC: C12M1/02 ,  C12M1/18

Abstract: Systems and methods for producing and using a body having a first structure defining a first chamber, a second structure defining a second chamber, a membrane located at an interface region between the first chamber and the second chamber to separate the first chamber from the second chamber. The first chamber comprises a first permeable matrix disposed therein and the first permeable matrix comprises at least one or a plurality of lumens each extending therethrough, which is optionally lined with at least one layer of cells. The second chamber can comprise cells cultured therein. The systems and methods described herein can be used for various applications, including, e.g., growth and/or differentiation of primary cells, and/or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents). The systems and methods can also permit co-cultures of two or more different cell types.

公开(公告)号：AU2016268134A1

公开(公告)日：2017-12-21

申请号：AU2016268134

申请日：2016-05-22

Applicant: HARVARD COLLEGE ,  CHILDRENS MEDICAL CT CORP

Inventor： INGBER DONALD E ,  JAIN ABHISHEK ,  VAN DER MEER ANDRIES D ,  MICHELSON ALAN DAVID ,  FRELINGER III ANDREW L ,  BARRILE RICCARDO

IPC: A61K35/19 ,  C12Q1/56 ,  G01N33/48 ,  G06F19/12

Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

公开(公告)号：AU2016249832A1

公开(公告)日：2017-12-07

申请号：AU2016249832

申请日：2016-04-08

Applicant: HARVARD COLLEGE

Inventor： DOMANSKY KAREL ,  HINOJOSA CHRISTOPHER DAVID ,  INGBER DONALD E ,  LEVNER DANIEL ,  THOMPSON II GUY

IPC: C12M1/00 ,  B82Y15/00 ,  C12M3/04

Abstract: A microfluidic device for determining a response of cells comprises a microchannel and a seeding channel. The microchannel is at least partially defined by a porous membrane having cells adhered thereto. The microchannel has a first cross-sectional area. The seeding channel delivers a working fluid to the cells within the microchannel. The seeding channel has a second cross-sectional area that is less than the first cross-sectional area such that a flow of the working fluid produces a substantially higher shear force within the seeding channel to inhibit the attachment of cells within the seeding channel. And when multiple seeding channels are used to deliver fluids to multiple microchannels that define an active cellular layer across the membrane, the seeding channels are spatially offset from each other such that fluid communication between the fluids occurs only at the active region via the membrane, not at the seeding channels.

公开(公告)号：HK1223560A1

公开(公告)日：2017-08-04

申请号：HK16111910

申请日：2016-10-14

Applicant: HARVARD COLLEGE

Inventor： SUPER MICHAEL ,  WATTERS ALEXANDER L ,  SNELL PHILIP T ,  INGBER DONALD E

IPC: C07K20060101 ,  A61K20060101