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    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    121.
    发明申请
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    光学系统和生物分析方法

    公开(公告)号：WO2013049709A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/058107

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence ,  CHENG, Francis

    IPC: G01N21/64

    CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

    Abstract: An instrument (1000) for processing and/or measuring a biological process contains an excitation source (110), a sample holder (204), an optical sensor (118), an excitation optical system (120), and an emission optical system (125). The sample holder (204) is configured to receive a plurality of biological samples. The optical sensor (118) is configured to receive an emission from the biological samples. The excitation optical system (120) is disposed along an excitation optical path (126) and is configured to direct the electromagnetic radiation from the excitation source (110) to the biological samples. The emission optical system (125) is disposed along an emission optical path (128) and is configured to direct electromagnetic emissions from the biological samples to the optical sensor (118). The instrument further contains a plurality of filter assemblies (130, 132) configured to be interchangeably located along at least one of the optical paths. The plurality of filter components (131) includes a first filter component (138) characterized by a first optical power and a first filter (140) having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly (142) is characterized by a second optical power and a second filter (145) having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength. The second optical power differs from the first optical power by an amount sufficient to at least partially compensate for an aberration introduced by the second filter (145) relative to the first filter (140).

    Abstract translation: 用于处理和/或测量生物过程的仪器（1000）包括激发源（110），样品保持器（204），光学传感器（118），激发光学系统（120）和发射光学系统 125）。 样品架（204）被配置为接收多个生物样品。 光学传感器（118）被配置为接收来自生物样品的发射。 激发光学系统（120）沿着激励光路（126）设置并且被配置为将来自激发源（110）的电磁辐射引导到生物样品。 发射光学系统（125）沿着发射光路（128）设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器（118）。 该仪器还包括多个过滤器组件（130,132），其被配置为沿着至少一个光路可互换地定位。 多个滤波器组件（131）包括具有第一光功率的第一滤波器组件（138）和具有第一滤波器功能的第一滤波器（140），第一滤波器功能的特征在于第一低通 波长或第一高通波长。 第二滤波器组件（142）的特征在于具有第二滤波器功能的第二光功率和第二滤波器（145），第二滤波器功能包括不同于第一低通滤波器的第二低通波长中的至少一个， 通过波长或与第一高通波长不同的第二高通波长。 第二光功率与第一光功率不同，足以至少部分地补偿由第二滤光器（145）相对于第一滤光器（140）引入的像差。

    AUTOMATED ON-INSTRUMENT pH ADJUSTMENT
    123.
    发明申请
    AUTOMATED ON-INSTRUMENT pH ADJUSTMENT 审中-公开
    自动化仪器pH调节

    公开(公告)号：WO2013012463A1

    公开(公告)日：2013-01-24

    申请号：PCT/US2012/032571

    申请日：2012-04-06

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  MARRAN, David ,  BEAUCHEMIN, Mark

    Inventor： MARRAN, David ,  BEAUCHEMIN, Mark

    IPC: G01N27/414

    CPC classification number: G01N33/84 ,  C12Q1/6874

    Abstract: A method of preparing a sequencing device includes determining a sensitivity of a pH of a solution to a first reagent, determining an amount of the first reagent to add to the solution to approach a target pH, adding the amount of the first reagent to the solution, and diluting a nucleotide solution with the solution.

    Abstract translation: 制备测序装置的方法包括确定溶液的pH对第一试剂的灵敏度，确定添加到溶液中的第一试剂的量以接近目标pH，将第一试剂的量加到溶液中 ，并用溶液稀释核苷酸溶液。

    NUCLEIC ACID COMPLEXITY REDUCTION
    124.
    发明申请
    NUCLEIC ACID COMPLEXITY REDUCTION 审中-公开
    核酸复杂性降低

    公开(公告)号：WO2013010062A2

    公开(公告)日：2013-01-17

    申请号：PCT/US2012/046624

    申请日：2012-07-13

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  GAVIN, Meredith ,  CLOUSER, Christopher ,  SOKOLSKY, Tanya ,  MATHER, Kimberly ,  MCKERNAN, Kevin ,  CALLAHAN, Marie ,  BEE, Gary

    Inventor： GAVIN, Meredith ,  CLOUSER, Christopher ,  SOKOLSKY, Tanya ,  MATHER, Kimberly ,  MCKERNAN, Kevin ,  CALLAHAN, Marie ,  BEE, Gary

    IPC: C12N15/10

    CPC classification number: C12Q1/6832 ,  C12Q2525/191 ,  C12Q2537/159 ,  C12Q2537/163

    Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for reducing the complexity of nucleotide sequences in a nucleic acid sample comprising the steps: hybridizing a plurality of polynucleotide constructs to at least one blocker oligonucleotide and to at least one capture oligonucleotide, wherein the plurality of polynucleotide constructs include a plurality of polynucleotides each joined to at least one nucleic acid adaptor, wherein the at least one nucleic acid adaptor can hybridize to the at least one blocker oligonucleotide, and wherein the at least one capture oligonucleotide can hybridize to at least a portion of target polynucleotides that are a sub-population of the plurality of polynucleotides, so as to produce a capture duplex.

    Abstract translation: 在一些实施方案中，本教导提供了用于降低核酸样品中核苷酸序列的复杂性的组合物，系统，方法和试剂盒，所述组合物，系统，方法和试剂盒包括以下步骤：将多个多核苷酸构建体与至少一种阻断剂 寡核苷酸和至少一种捕获寡核苷酸，其中所述多种多核苷酸构建体包括多种多核苷酸，每种多核苷酸与至少一种核酸衔接子连接，其中所述至少一种核酸衔接子可以与所述至少一种阻断剂寡核苷酸杂交，并且其中 所述至少一种捕获寡核苷酸可与作为所述多种多核苷酸的亚群的靶多核苷酸的至少一部分杂交，从而产生捕获双链体。

    POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME
    125.
    发明申请
    POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME 审中-公开
    聚合物颗粒，核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：WO2013006824A2

    公开(公告)日：2013-01-10

    申请号：PCT/US2012/045827

    申请日：2012-07-06

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIGHT, David ,  ROSENBLUM, Barnett

    Inventor： LIGHT, David ,  ROSENBLUM, Barnett

    IPC: C08F2/32 ,  C12Q1/68 ,  C08F220/56 ,  C08F6/00

    CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

    Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol% to about 20 vol%. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

    Abstract translation: 本公开涉及制备聚合物颗粒的方法，所述方法包括以下步骤：制备水性凝胶反应混合物; 在连续相中形成具有分散的凝胶反应混合物的水相胶束的乳液; 向所述连续相中加入包含至少一种聚合引发剂的引发剂油; 并在胶束中进行聚合反应。 此外，引发剂油相对于水性凝胶反应混合物的体积的体积％为约1体积％至约20体积％。 本公开还涉及制备具有相同方法步骤的核酸聚合物颗粒的方法，并且其中所述水性凝胶反应混合物包括核酸片段，例如引物。

    COMPOSITIONS AND METHODS FOR STABILIZING SUSCEPTIBLE COMPOUNDS
    130.
    发明申请
    COMPOSITIONS AND METHODS FOR STABILIZING SUSCEPTIBLE COMPOUNDS 审中-公开
    用于稳定可疑化合物的组合物和方法

    公开(公告)号：WO2012109279A2

    公开(公告)日：2012-08-16

    申请号：PCT/US2012/024194

    申请日：2012-02-07

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  FIKE, Richard ,  BRANCHAUD, Bruce ,  BARRETT, Shawn

    Inventor： FIKE, Richard ,  BRANCHAUD, Bruce ,  BARRETT, Shawn

    IPC: C12N5/00

    CPC classification number: C12N5/0018 ,  C12N2500/34 ,  C12N2500/46

    Abstract: Methods for increasing the stability of, or protecting, labile components such as ethanolamine, growth factors, vitamins, etc., in compositions such as a cell culture medium. Stability of the labile compound is increased either, by derivatization of the labile compound with chemicals, or by sequestering the labile compound. Sequestering can be done either by encapsulation within a microcapsule, or by the use of sequestering agents. Encapsulation includes the encapsulation of dendrimers complexes of susceptible compounds within the microcapsule, thereby providing the controlled release of the susceptible compound that was protected.These methods may improve and extend storage conditions of compositions comprising the labile compounds, improve shipping and handling of compositions comprising the labile compounds, such as dry media formulations, at room temperature rather than at lower temperatures thereby decreasing shipping costs. Stabilization of labile compounds in compositions such as dry format media can be viewed as a contribution to green technology.

    Abstract translation: 在诸如细胞培养基的组合物中增加不稳定组分如乙醇胺，生长因子，维生素等的稳定性或保护的方法。 不稳定化合物的稳定性也通过不稳定化合物与化学品的衍生化或通过螯合不稳定化合物而增加。 螯合可以通过在微胶囊内包封或通过使用螯合剂进行。 封装包括在微胶囊中包封易感化合物的树枝状大分子复合物，从而提供受保护的易感化合物的受控释放。这些方法可以改善和延长包含不稳定化合物的组合物的储存条件，改善包含 不稳定的化合物，例如干介质制剂，而不是在较低的温度下，从而降低运输成本。 不稳定化合物在组合物如干式培养基中的稳定可被视为对绿色技术的贡献。

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