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    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    1.
    发明申请
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    光学系统和生物分析方法

    公开(公告)号：WO2013049709A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/058107

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence ,  CHENG, Francis

    IPC: G01N21/64

    CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

    Abstract: An instrument (1000) for processing and/or measuring a biological process contains an excitation source (110), a sample holder (204), an optical sensor (118), an excitation optical system (120), and an emission optical system (125). The sample holder (204) is configured to receive a plurality of biological samples. The optical sensor (118) is configured to receive an emission from the biological samples. The excitation optical system (120) is disposed along an excitation optical path (126) and is configured to direct the electromagnetic radiation from the excitation source (110) to the biological samples. The emission optical system (125) is disposed along an emission optical path (128) and is configured to direct electromagnetic emissions from the biological samples to the optical sensor (118). The instrument further contains a plurality of filter assemblies (130, 132) configured to be interchangeably located along at least one of the optical paths. The plurality of filter components (131) includes a first filter component (138) characterized by a first optical power and a first filter (140) having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly (142) is characterized by a second optical power and a second filter (145) having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength. The second optical power differs from the first optical power by an amount sufficient to at least partially compensate for an aberration introduced by the second filter (145) relative to the first filter (140).

    Abstract translation: 用于处理和/或测量生物过程的仪器（1000）包括激发源（110），样品保持器（204），光学传感器（118），激发光学系统（120）和发射光学系统 125）。 样品架（204）被配置为接收多个生物样品。 光学传感器（118）被配置为接收来自生物样品的发射。 激发光学系统（120）沿着激励光路（126）设置并且被配置为将来自激发源（110）的电磁辐射引导到生物样品。 发射光学系统（125）沿着发射光路（128）设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器（118）。 该仪器还包括多个过滤器组件（130,132），其被配置为沿着至少一个光路可互换地定位。 多个滤波器组件（131）包括具有第一光功率的第一滤波器组件（138）和具有第一滤波器功能的第一滤波器（140），第一滤波器功能的特征在于第一低通 波长或第一高通波长。 第二滤波器组件（142）的特征在于具有第二滤波器功能的第二光功率和第二滤波器（145），第二滤波器功能包括不同于第一低通滤波器的第二低通波长中的至少一个， 通过波长或与第一高通波长不同的第二高通波长。 第二光功率与第一光功率不同，足以至少部分地补偿由第二滤光器（145）相对于第一滤光器（140）引入的像差。

    METHODS FOR THE ANALYSIS OF DISSOCIATION MELT CURVE DATA
    2.
    发明申请
    METHODS FOR THE ANALYSIS OF DISSOCIATION MELT CURVE DATA 审中-公开
    分析曲线曲线数据分析方法

    公开(公告)号：WO2009094658A1

    公开(公告)日：2009-07-30

    申请号：PCT/US2009/032049

    申请日：2009-01-26

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  CHENG, Francis ,  MC FARLAND, Casey

    Inventor： CHENG, Francis ,  MC FARLAND, Casey

    IPC: C12Q1/68

    CPC classification number: G06F17/5009 ,  C12Q1/6816 ,  G06F19/18 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

    Abstract translation: 提供了对原始解离数据和解离曲线进行操作以产生检测数据的校准并进一步改进数据分析的方法。 可以从多区域平台的每个支撑区域（例如，从多孔板的每个孔）获取数据。 在运行使用这种样品的实验之前，每个支撑区域可以装载相同样品的部分。 在一些实施方案中，提供了一种产生解析曲线的熔融过渡区域的方法，其显示样品的熔融特性。 在一些实施方案中，可以对解离曲线数据进行染料温度依赖性校正，以进一步改进分析。 在一些实施例中，可以从熔体数据导出特征向量，并且可以使用特征向量来进一步改进解离曲线的基因分型分析。

    METHODS FOR THE ANALYSIS OF DISSOCIATION MELT CURVE DATA
    4.
    发明公开
    METHODS FOR THE ANALYSIS OF DISSOCIATION MELT CURVE DATA 有权
    方法用于分析解离解链曲线数据

    公开(公告)号：EP2262912A1

    公开(公告)日：2010-12-22

    申请号：EP09704227.9

    申请日：2009-01-26

    Applicant: Life Technologies Corporation

    Inventor： CHENG, Francis ,  MC FARLAND, Casey

    IPC: C12Q1/68

    CPC classification number: G06F17/5009 ,  C12Q1/6816 ,  G06F19/18 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

    METHODS FOR THE ANALYSIS OF DISSOCIATION MELT CURVE DATA
    5.
    发明公开
    METHODS FOR THE ANALYSIS OF DISSOCIATION MELT CURVE DATA 审中-公开
    分离熔融曲线数据的方法

    公开(公告)号：EP3037551A1

    公开(公告)日：2016-06-29

    申请号：EP15181948.9

    申请日：2009-01-26

    Applicant: Life Technologies Corporation

    Inventor： CHENG, Francis ,  MC FARLAND, Casey

    IPC: C12Q1/68

    Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

    Abstract translation: 提供的方法根据原始解离数据和解离曲线进行操作，以生成检测数据的校准并进一步改进数据分析。 数据可以从多区域平台的每个支持区域取得，例如从多孔板的每个孔取得。 在使用这样的样品进行实验之前，每个支撑区域可以装载相同样品的部分。 在一些实施方案中，提供了用于产生解离曲线的解链过渡区的方法，其显示了样品的解链特征。 在一些实施例中，可以对解离曲线数据执行染料温度依赖性校正以进一步改进分析。 在一些实施例中，特征向量可以从熔化数据导出，并且特征向量可以用于进一步改善解离曲线的基因分型分析。

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