公开(公告)号：WO2015134925A1

公开(公告)日：2015-09-11

申请号：PCT/US2015/019280

申请日：2015-03-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAHER, Kevin

IPC: G01N27/447

CPC classification number: G01N27/44721 ,  G01N21/645 ,  G01N27/44791 ,  G01N2021/6417 ,  G01N2021/6484 ,  G01N2201/062

Abstract: A system for conducting a capillary electrophoresis assay includes a light source, an interface, and an illumination optical system. The light source is configured to provide a source beam of electromagnetic radiation. The interface is configured to receive a plurality of capillaries containing one or more target molecules or sequence of molecules. The illumination optical system is configured in use to produce a plurality of output beams from the source beam and to direct each of the output beams to corresponding capillary of the plurality of capillaries.

Abstract translation: 用于进行毛细管电泳测定的系统包括光源，界面和照明光学系统。 光源被配置为提供电磁辐射的源束。 界面被配置为接收包含一个或多个靶分子或分子序列的多个毛细管。 照明光学系统被配置为用于从源光束产生多个输出光束并且将每个输出光束引导到多个毛细管中的相应毛细管。

公开(公告)号：WO2013138767A1

公开(公告)日：2013-09-19

申请号：PCT/US2013/032420

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PALLAS, Michael, C. ,  NURSE, James ,  LIM, Gary ,  STRAUB, Theodore ,  GHENCIU, Eliodor ,  FOSTER, Evan ,  FONSECA, Jorge ,  MAHER, Kevin

IPC: B01L7/00 ,  C12Q1/68 ,  B01L3/00

CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L2200/141 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，该芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。

公开(公告)号：WO2013049709A1

公开(公告)日：2013-04-04

申请号：PCT/US2012/058107

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence ,  CHENG, Francis

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

Abstract: An instrument (1000) for processing and/or measuring a biological process contains an excitation source (110), a sample holder (204), an optical sensor (118), an excitation optical system (120), and an emission optical system (125). The sample holder (204) is configured to receive a plurality of biological samples. The optical sensor (118) is configured to receive an emission from the biological samples. The excitation optical system (120) is disposed along an excitation optical path (126) and is configured to direct the electromagnetic radiation from the excitation source (110) to the biological samples. The emission optical system (125) is disposed along an emission optical path (128) and is configured to direct electromagnetic emissions from the biological samples to the optical sensor (118). The instrument further contains a plurality of filter assemblies (130, 132) configured to be interchangeably located along at least one of the optical paths. The plurality of filter components (131) includes a first filter component (138) characterized by a first optical power and a first filter (140) having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly (142) is characterized by a second optical power and a second filter (145) having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength. The second optical power differs from the first optical power by an amount sufficient to at least partially compensate for an aberration introduced by the second filter (145) relative to the first filter (140).

Abstract translation: 用于处理和/或测量生物过程的仪器（1000）包括激发源（110），样品保持器（204），光学传感器（118），激发光学系统（120）和发射光学系统 125）。 样品架（204）被配置为接收多个生物样品。 光学传感器（118）被配置为接收来自生物样品的发射。 激发光学系统（120）沿着激励光路（126）设置并且被配置为将来自激发源（110）的电磁辐射引导到生物样品。 发射光学系统（125）沿着发射光路（128）设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器（118）。 该仪器还包括多个过滤器组件（130,132），其被配置为沿着至少一个光路可互换地定位。 多个滤波器组件（131）包括具有第一光功率的第一滤波器组件（138）和具有第一滤波器功能的第一滤波器（140），第一滤波器功能的特征在于第一低通 波长或第一高通波长。 第二滤波器组件（142）的特征在于具有第二滤波器功能的第二光功率和第二滤波器（145），第二滤波器功能包括不同于第一低通滤波器的第二低通波长中的至少一个， 通过波长或与第一高通波长不同的第二高通波长。 第二光功率与第一光功率不同，足以至少部分地补偿由第二滤光器（145）相对于第一滤光器（140）引入的像差。

公开(公告)号：WO2014026156A1

公开(公告)日：2014-02-13

申请号：PCT/US2013/054413

申请日：2013-08-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAHER, Kevin

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6452 ,  G01N2021/6463 ,  G01N2201/0446 ,  G01N2201/062 ,  G01N2201/0631

Abstract: A system (500) for detecting emissions from a sample containing nucleotide molecules contains a sample holder (501), an optical illumination system (612), and an optical sensor (620). The sample holder includes a plurality of spatially separated reaction sites configured to hold a sample containing nucleotide molecules. The optical illumination system comprises a radiant source (610) configured to simultaneously illuminate two or more of the reaction sites. The illumination system includes a homogenizer (112). An output from the homogenizer has less variation in power, energy, irradiance, or intensity than the variation in power, energy, irradiance, or intensity of the source.

Abstract translation: 用于检测含有核苷酸分子的样品的发射的系统（500）包含样品保持器（501），光学照明系统（612）和光学传感器（620）。 样品架包括多个空间分离的反应位点，其配置成容纳含有核苷酸分子的样品。 光学照明系统包括被配置为同时照亮两个或更多个反应位点的辐射源（610）。 照明系统包括均化器（112）。 来自均化器的输出与功率，能量，辐照度或源的强度的变化相比，功率，能量，辐照度或强度的变化较小。

公开(公告)号：WO2013138706A2

公开(公告)日：2013-09-19

申请号：PCT/US2013/032002

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PALLAS, Michael ,  NURSE, James C. ,  MAHER, Kevin ,  FONSECA, Jorge ,  GHENCIU, Eliodor ,  FOSTER, Evan

IPC: B01L3/00

CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L2200/141 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

Abstract: An article for holding a plurality of biological samples includes a substrate a substrate comprising a first surface and an opposing second surface and a plurality of reaction sites in the substrate. Each of the reaction sites extends from an opening in the first surface to an opening in the second surface. The reaction sites comprise a hexagonal shape and are configured to provide sufficient surface tension by capillary action to hold respective biological samples. The reaction sites have a density over at least a portion of the surfaces that is at least 170 holes per square millimeter. At least one of the surfaces may have a surface roughness characterized by an arithmetic average roughness (Ra) that is less than or equal to 5 nanometers.

Abstract translation: 用于保持多个生物样品的制品包括基底，该基底包括基底中的第一表面和相对的第二表面和多个反应位点。 每个反应位置从第一表面中的开口延伸到第二表面中的开口。 反应位点包括六边形形状，并且构造成通过毛细作用提供足够的表面张力以保持各自的生物样品。 反应位点的表面密度至少在每平方毫米170个孔以上。 至少一个表面可以具有由小于或等于5纳米的算术平均粗糙度（Ra）表征的表面粗糙度。

公开(公告)号：WO2013138685A1

公开(公告)日：2013-09-19

申请号：PCT/US2013/031890

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAHER, Kevin

IPC: B01L7/00 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/50857 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0893 ,  B01L2300/0896 ,  C12Q2563/159 ,  C12Q2565/619

Abstract: A system for determining the number of target nucleotide molecules in a sample includes a sample holder, an excitation optical system, an optical sensor, and an emission optical system. The sample holder is configured to receive an article comprising at least 20,000 separate reaction sites. The excitation optical system comprises a light source configured to simultaneously illuminate the at least 20,000 separate reaction sites. The optical sensor comprises a predetermined number of pixels, the predetermined number of pixels being at least 20 times the number of separate reaction sites. The emission optical system comprises a system working distance from the sample holder, wherein the working distance is less than or equal to 60 millimeters.

Abstract translation: 用于确定样品中目标核苷酸分子数目的系统包括样品架，激发光学系统，光学传感器和发射光学系统。 样品架被配置为接收包含至少20,000个单独的反应位点的制品。 激发光学系统包括被配置为同时照亮至少20,000个单独的反应位点的光源。 光学传感器包括预定数量的像素，预定数量的像素是分离反应位点数量的至少20倍。 发射光学系统包括与样品保持器的系统工作距离，其中工作距离小于或等于60毫米。

公开(公告)号：WO2013049702A9

公开(公告)日：2013-04-04

申请号：PCT/US2012/058096

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： FREUDENTHAL, Jacob K. ,  DAHLHOFF, Geoffrey ,  MAHER, Kevin ,  CHEN, Mingsong ,  LIM, Gary ,  DANEHY, Ronald ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang Terence ,  CHENG, Francis ,  WU, Ruoyun ,  LEE, Thiam Chye ,  CHAPPELL, Gary ,  LIM, Chee Kiong ,  DERE, Jeff ,  STRAUB, Ted ,  FONSECA, Jorge ,  BOO, Kuan Moon ,  LAU, Soo Yong

IPC: B01L7/00 ,  B01L3/00 ,  C12Q1/68

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lens can be a bi-convex lens.

公开(公告)号：EP3417941A1

公开(公告)日：2018-12-26

申请号：EP18172310.7

申请日：2013-03-15

Applicant: Life Technologies Corporation

Inventor： MAHER, Kevin

IPC: B01L7/00 ,  C12Q1/68 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  B01L3/50857 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0893 ,  B01L2300/0896 ,  C12Q2563/159 ,  C12Q2565/619

Abstract: A system for determining the number of target nucleotide molecules in a sample includes a sample holder, an excitation optical system, an optical sensor, and an emission optical system. The sample holder is configured to receive an article comprising at least 20,000 separate reaction sites. The excitation optical system comprises a light source configured to simultaneously illuminate the at least 20,000 separate reaction sites. The optical sensor comprises a predetermined number of pixels, the predetermined number of pixels being at least 20 times the number of separate reaction sites. The emission optical system comprises a system working distance from the sample holder, wherein the working distance is less than or equal to 60 millimeters.

公开(公告)号：EP2761277A1

公开(公告)日：2014-08-06

申请号：EP12783710.2

申请日：2012-09-28

Applicant: Life Technologies Corporation

Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

Abstract: An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. The optical sensor is configured to receive an emission from the biological samples. The excitation optical system is disposed along an excitation optical path and is configured to direct the electromagnetic radiation from the excitation source to the biological samples. The emission optical system is disposed along an emission optical path and is configured to direct electromagnetic emissions from the biological samples to the optical sensor. The instrument further contains a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.

Abstract translation: 用于处理和/或测量生物过程的仪器包含激发源，样品架，光学传感器，激发光学系统和发射光学系统。 样品架配置成接收多个生物样品。 光学传感器被配置为接收来自生物样品的发射。 激发光学系统沿着激发光学路径设置并且被配置为将来自激发源的电磁辐射引导到生物样品。 发射光学系统沿着发射光路设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器。 仪器还包括多个过滤器组件，其被配置为沿着至少一个光路可互换地定位。 多个滤波器部件包括第一滤波器组件，其特征在于第一光功率和具有第一滤波器功能的第一滤波器，第一滤波器功能的特征在于第一低通波长或第一高通波长中的至少一个。 第二滤波器组件的特征在于具有第二滤波器功能的第二光功率和第二滤波器，第二滤波器功能包括与第一低通波长不同的第二低通波长或第二高通波长中的至少一个 通过波长不同于第一高通波长。

公开(公告)号：EP4517318A2

公开(公告)日：2025-03-05

申请号：EP24213815.4

申请日：2015-03-06

Applicant: Life Technologies Corporation

Inventor： MAHER, Kevin

IPC: G01N27/447

Abstract: A system for conducting a capillary electrophoresis assay includes a light source, an interface, and an illumination optical system. The light source is configured to provide a source beam of electromagnetic radiation. The interface is configured to receive a plurality of capillaries containing one or more target molecules or sequence of molecules. The illumination optical system is configured in use to produce a plurality of output beams from the source beam and to direct each of the output beams to corresponding capillary of the plurality of capillaries.