中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

23 records (took 0.039 seconds)

    SYSTEM AND METHODS FOR CALIBRATING BINDING DYES
    2.
    发明申请
    SYSTEM AND METHODS FOR CALIBRATING BINDING DYES 审中-公开
    用于校准结合染色体的系统和方法

    公开(公告)号：WO2016127080A1

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016817

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHU, Yong ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

    IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68

    CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/127

    Abstract: A computer-implemented method (500) for calibrating a fluorescent dye is described. The method (500) comprises imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder comprises a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method further comprises identifying a peak channel for each dye on the sample holder (508), normalizing each channel to the peak channel for each dye (510), and producing a dye matrix (518) that comprises a set of dye reference values.

    Abstract translation: 描述了用于校准荧光染料的计算机实现的方法（500）。 方法（500）包括在多个通道上成像加载到仪器中的样品保持器。 样品架包括多个反应位点和多于一种染料类型，每种染料占据多于一个反应位点。 该方法还包括鉴定样品保持器（508）上的每种染料的峰值通道，将每个通道标准化为每个染料（510）的峰值通道，以及产生包含一组染料参考值的染料基质（518）。

    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    3.
    发明申请
    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    生物分析系统与方法

    公开(公告)号：WO2013049659A2

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/058042

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： DANEHY, Ronald ,  LIM, Gary ,  FREUDENTHAL, Jacob

    IPC: B01L3/00 ,  C12M1/00

    CPC classification number: C12Q1/6806 ,  B01L3/50851 ,  B01L3/50855 ,  B01L3/50857 ,  B01L2200/026 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0893 ,  B01L2300/161 ,  C12M23/12 ,  C12M23/38

    Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.

    Abstract translation: 用于容纳多个生物样品的壳体包含基底，基底和盖。 底物附着到基底上，并且包括被配置成接收一个或多个生物样品的多个反应区域。 盖子包括内表面，并且被构造成附接到基座，使得基座和盖子一起提供容纳基底的空腔。 内表面包括中心区域和围绕中心区域设置的凹陷区域。 中心区域构造成覆盖反应区域，而凹陷区域在垂直于内表面的方向上偏离中心区域。

    METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION
    4.
    发明申请
    METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION 审中-公开
    仪器验证的方法和系统

    公开(公告)号：WO2016127032A1

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016730

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： WESSEL, Thomas ,  CHU, Yong ,  FREUDENTHAL, Jacob ,  WOO, David

    IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/26

    CPC classification number: G01N21/6428 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G06F19/24

    Abstract: A method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves (102, 202). The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves (104, 204) and determining, for each fluorescence threshold of the set, a first set of cycle threshold (C t ) values of amplification curves generated from the samples of the first quantity and a second set of C t values of amplification curves generated from the samples of the second quantity (106, 206). The method includes calculating if the first and second quantities are sufficiently distinguishable based on C t values at each of the plurality of fluorescence thresholds (108, 208-218).

    Abstract translation: 提供了一种验证仪器的方法。 该方法包括从验证板接收放大数据以产生多个放大曲线（102,202）。 验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。 该方法还包括基于多个扩增曲线（104,204）的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定放大曲线的第一组周期阈值（Ct）值 从第一数量的样本和从第二数量（106,206）的样本生成的第二组扩增曲线的Ctval值产生。 该方法包括基于多个荧光阈值（108,208-218）中的每一个的Ct值来计算第一和第二量是否足够可区分。

    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    5.
    发明申请
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    光学系统和生物分析方法

    公开(公告)号：WO2014130970A2

    公开(公告)日：2014-08-28

    申请号：PCT/US2014/018110

    申请日：2014-02-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHEN, Mingsong ,  AGUANNO, Mauro ,  BOO, Kuan Moon ,  SOH, Woon Liang Terence ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

    IPC: G01N21/27

    CPC classification number: G01N21/6486 ,  G01N21/0332 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2201/061 ,  G01N2201/0624 ,  G01N2201/0627 ,  G01N2201/121

    Abstract: An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. The instrument also includes an excitation source temperature controller comprising a temperature sensor that is coupled to the excitation source. The temperature controller is configured to produce a first target temperature when the first sample holder is retained by the instrument and to produce a second target temperature when the second sample holder is retained by the instrument.

    Abstract translation: 用于处理和/或测量生物过程的仪器包括样品处理系统，激发源，激发光学系统，光学传感器和发射光学系统。 样品处理系统被配置为保持第一样品保持器和第二样品保持器，其中样品池的数量对于每个样品保持器是不同的，或者第一样品池的特征尺寸与第二样品保持器的特征尺寸不同。 仪器还包括激发源温度控制器，其包括耦合到激发源的温度传感器。 温度控制器被配置为当第一样品保持器被仪器保持时产生第一目标温度，并且当第二样品保持器被仪器保持时产生第二目标温度。

    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    6.
    发明申请
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    光学系统和生物分析方法

    公开(公告)号：WO2013049709A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/058107

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence ,  CHENG, Francis

    IPC: G01N21/64

    CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

    Abstract: An instrument (1000) for processing and/or measuring a biological process contains an excitation source (110), a sample holder (204), an optical sensor (118), an excitation optical system (120), and an emission optical system (125). The sample holder (204) is configured to receive a plurality of biological samples. The optical sensor (118) is configured to receive an emission from the biological samples. The excitation optical system (120) is disposed along an excitation optical path (126) and is configured to direct the electromagnetic radiation from the excitation source (110) to the biological samples. The emission optical system (125) is disposed along an emission optical path (128) and is configured to direct electromagnetic emissions from the biological samples to the optical sensor (118). The instrument further contains a plurality of filter assemblies (130, 132) configured to be interchangeably located along at least one of the optical paths. The plurality of filter components (131) includes a first filter component (138) characterized by a first optical power and a first filter (140) having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly (142) is characterized by a second optical power and a second filter (145) having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength. The second optical power differs from the first optical power by an amount sufficient to at least partially compensate for an aberration introduced by the second filter (145) relative to the first filter (140).

    Abstract translation: 用于处理和/或测量生物过程的仪器（1000）包括激发源（110），样品保持器（204），光学传感器（118），激发光学系统（120）和发射光学系统 125）。 样品架（204）被配置为接收多个生物样品。 光学传感器（118）被配置为接收来自生物样品的发射。 激发光学系统（120）沿着激励光路（126）设置并且被配置为将来自激发源（110）的电磁辐射引导到生物样品。 发射光学系统（125）沿着发射光路（128）设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器（118）。 该仪器还包括多个过滤器组件（130,132），其被配置为沿着至少一个光路可互换地定位。 多个滤波器组件（131）包括具有第一光功率的第一滤波器组件（138）和具有第一滤波器功能的第一滤波器（140），第一滤波器功能的特征在于第一低通 波长或第一高通波长。 第二滤波器组件（142）的特征在于具有第二滤波器功能的第二光功率和第二滤波器（145），第二滤波器功能包括不同于第一低通滤波器的第二低通波长中的至少一个， 通过波长或与第一高通波长不同的第二高通波长。 第二光功率与第一光功率不同，足以至少部分地补偿由第二滤光器（145）相对于第一滤光器（140）引入的像差。

    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    7.
    发明申请
    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开

    公开(公告)号：WO2022020729A2

    公开(公告)日：2022-01-27

    申请号：PCT/US2021/042997

    申请日：2021-07-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： FREUDENTHAL, Jacob ,  BENSON, Scott

    IPC: G01N21/64 ,  C12Q1/686 ,  C12Q1/6851 ,  G01J3/4406 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6456

    Abstract: 1. A system (1000), comprising: a radiant source (101) characterized by an average excitation wavelength; a sample (110) disposed to receive radiation from the radiant source, the sample comprising:a first dye;a second dye; and a detector (115) configured to measure emissions from the sample; a first emission spectral element (121a) characterized by a first average emission wavelength; a second emission spectral element (121b) characterized by a second average emission wavelength that is different than the first average emission wavelength; at least one processor (130) comprising at least one memory including instructions to: illuminate the sample with the radiant source and, in response, (1) measure emissions from the sample using the detector and the first emission spectral element and (2) measure emissions from the sample using the detector and the second emission spectral element. A method corresponding to the above system.

    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION
    8.
    发明申请
    METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开
    生物仪器校准的方法和系统

    公开(公告)号：WO2016127124A2

    公开(公告)日：2016-08-11

    申请号：PCT/US2016/016882

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHU, Yong ,  MARKS, Jeffrey ,  FREUDENTHAL, Jacob ,  WESSEL, Thomas ,  WOO, David

    IPC: G01N21/27 ,  G01N21/64

    CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

    Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

    Abstract translation: 在一个示例性实施例中，提供了一种用于校准仪器的方法。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。 该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准以确定滤波器归一化因子。 该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

Patent Agency Ranking