公开(公告)号：DE502004008546D1

公开(公告)日：2009-01-08

申请号：DE502004008546

申请日：2004-09-23

Applicant: LEICA MICROSYSTEMS

Inventor： ULRICH HEINRICH ,  KNEBEL WERNER ,  MOELLMANN KYRA

IPC: G02B21/00 ,  G02B21/02 ,  G02B21/06 ,  G02B21/08 ,  G02B21/32

Abstract: A scanning microscope includes a light source for evanescently illuminating a sample disposed on a slide. A point detector receives detection light emanating from a scanning point of the sample. A beam deflection device disposed in an optical path of the detection light can shift a position of the scanning point.

公开(公告)号：DE102006045607A1

公开(公告)日：2008-03-27

申请号：DE102006045607

申请日：2006-09-25

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER ,  SCHROEDER JAN

IPC: G02B21/00 ,  G01N21/64

Abstract: The research process involves using a target protein (5) in living cells as the structure which is marked with the fluorescent substance (3). A ligand complex (1) including the fluorescent substance is bonded to and enzyme (4) by an enzymatic reaction in the cell. The enzyme is expelled as a fusion protein (6) together with the target protein.

公开(公告)号：DE502004003515D1

公开(公告)日：2007-05-31

申请号：DE502004003515

申请日：2004-07-21

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  KNEBEL WERNER

IPC: G02B21/00 ,  G02B21/06

Abstract: The scanning microscope (1) has an incoupling apparatus (31) with which light (33) other than the light (17) proceeding from the sample (7) is coupled into the detection beam path and conveyed to the detector (21). A closure apparatus automatically closes off the incoupling apparatus from the outside, in largely light-tight fashion, when the light guide (37) guiding the other light to the incoupling apparatus is removed. An independent claim is also included for confocal scanning microscope.

公开(公告)号：DE10251345B4

公开(公告)日：2006-08-17

申请号：DE10251345

申请日：2002-11-05

Applicant: LEICA MICROSYSTEMS

Inventor： HOPPE MARTIN ,  KNEBEL WERNER ,  MOELLMANN KYRA ,  STORZ RAFAEL

IPC: A61B5/00 ,  A61B5/02 ,  G02B21/24

Abstract: The invention concerns a method and an apparatus for investigating layers ( 1 ) of tissues in living animals using a microscope ( 2 ). The microscope ( 2 ) is focused onto a layer ( 1 ), and images of the layer ( 1 ) are acquired or optical measurements are performed on it. Positional changes of the layer ( 1 ) are brought about by movements of the animal or of its organs. The positional changes are sensed, and corresponding signals are generated. The signals are stored, together with the corresponding images or measurement results, for later evaluation; or they are processed in such a way that the positional changes are compensated for in order to investigate the layer ( 1 ). As a result, the layer ( 1 ) can be qualitatively or quantitatively investigated microscopically, irrespective of the movement of the animal or its organs.

公开(公告)号：DE60205064T2

公开(公告)日：2006-06-01

申请号：DE60205064

申请日：2002-05-16

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER ,  WAWROWSKY KOLJA

IPC: G02B21/00 ,  G02B21/06

Abstract: The present invention concerns a method and an apparatus for for ROI-scan with high temporal resolution of a specimen (11).At least one light source (1, 2) generats an illumination light beam (4) to be scanned by a scanning device (9) across the specimen (11). The scan pattern (23) and at least one region of interest defines a first plurality of first scan lines (21) and a second plurality of second scan lines (22).Means for adjusting illumination conditions of the at least one light source are positioned in the illumination beam path prior to the scan device (9).Control means connected to the scan device (9) and the means for adjusting the illumination conditions are responsive to the position of the light beam on the specimen.

公开(公告)号：DE102004031048A1

公开(公告)日：2006-01-12

申请号：DE102004031048

申请日：2004-06-25

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER ,  SEYFRIED VOLKER ,  SCHROEDER JAN

IPC: G02B21/00 ,  G02B21/06 ,  G02B21/16

Abstract: A microscope includes at least one illuminating light source and an illumination beam path for guiding the illuminating light to a specimen. A detection beam path guides detected light from the specimen to a detector. An activatable element is positioned in the detection beam path for regulating and/or limiting the light power level in the detection beam path.

公开(公告)号：DE102004016253A1

公开(公告)日：2005-11-10

申请号：DE102004016253

申请日：2004-04-02

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER

IPC: G02B21/00

Abstract: A scanning microscope including a light source for generating an illumination light beam, a beam deflection apparatus for guiding solely the illumination light beam via an illumination light path over and/or through a sample, at least one objective for focusing the illumination light beam onto and/or into the sample, components for generating a manipulation illumination pattern, components for imaging the manipulation illumination pattern onto and/or into the sample via a manipulation light path, and a detection device that receives detected light emanating from the sample. The manipulation light path omits the beam deflection apparatus and is separate from the illumination light path.

公开(公告)号：DE10213187A1

公开(公告)日：2003-10-09

申请号：DE10213187

申请日：2002-03-23

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER

IPC: G01J3/32 ,  G01J3/36 ,  G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/36

Abstract: A method for spectral analysis of the light proceeding from a specimen using a multi-band detector comprises the steps of defining an overall spectral region; from the overall spectral region, defining a first spectral subregion and defining at least a second spectral subregion; simultaneously detecting the light proceeding from the specimen in the first and the second spectral subregion, and generating detection values; displacing the first spectral subregion and displacing the second spectral subregion within the overall spectral region; and repeating steps c) and d) until the light has been detected over the entire overall spectral region.

公开(公告)号：GB2369739B

公开(公告)日：2002-11-20

申请号：GB0123392

申请日：2001-09-28

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  KNEBEL WERNER

IPC: G02B26/10 ,  G02B21/00 ,  G02B21/06 ,  G02B21/24 ,  G02B21/36 ,  G06T1/00

Abstract: A method and an arrangement for beam control in a scanning microscope are disclosed. The scanning microscope comprises means for acquiring and displaying (3) a preview image (7) and a microscope optical system (51). Means for marking (5) at least one region of interest (27, 29) in the preview image (7) are provided. A first beam deflection device (43, 67, 68) displaces the scan field (31, 33) onto the region of interest (27, 29); and a second beam deflection device (49, 72, 94) serves for meander-shaped scanning within the scan field (31, 33).

公开(公告)号：DE10058100A1

公开(公告)日：2002-06-06

申请号：DE10058100

申请日：2000-11-23

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  KNEBEL WERNER

IPC: G02B21/00

Abstract: A portion of a specimen region to be examined with scan field, is scanned. The specimen stage in the X-Y plane is moved to scan the remaining portions of the specimen region, such that the entire specimen region is located within the scan fields. The specimen data obtained from all the scan fields are combined. An Independent claim is included for microscopic specimen scanning arrangement.