公开(公告)号：US5938617A

公开(公告)日：1999-08-17

申请号：US760165

申请日：1996-12-03

Applicant: Tuan Vo-Dinh

Inventor： Tuan Vo-Dinh

IPC: A61B5/00 ,  G01J3/12 ,  G01N21/64 ,  A61B6/00

CPC classification number: G01N21/6402 ,  A61B5/0059 ,  G01J3/1256 ,  G01N21/6456 ,  G01N21/6486

Abstract: A method and apparatus for detecting and identifying biological pathogens in a sample includes exposing the sample to an excitation radiation and thereby generating an emission radiation, synchronously scanning the wavelength of the excitation radiation and the wavelength of the emission radiation to produce a spectrum, and correlating the spectrum to a biological pathogen in the sample. In another aspect, a method and apparatus for imaging chemical and biological agents in a sample includes exposing the sample to an excitation radiation and thereby generating an emission radiation and synchronously imaging the wavelength of the excitation radiation and the wavelength of the emission radiation to produce an imaged spectrum.

Abstract translation: 用于检测和识别样品中生物病原体的方法和装置包括将样品暴露于激发辐射，从而产生发射辐射，同步扫描激发辐射的波长和发射辐射的波长以产生光谱，并且将相关 样品中生物病原体的光谱。 在另一方面，用于对样品中的化学和生物试剂进行成像的方法和装置包括将样品暴露于激发辐射，从而产生发射辐射并同步成像激发辐射的波长和发射辐射的波长，以产生 成像光谱。

公开(公告)号：US5912165A

公开(公告)日：1999-06-15

申请号：US942549

申请日：1997-10-01

Applicant: Dario Cabib ,  Robert A. Buckwald ,  Nissim Ben-Yosef

Inventor： Dario Cabib ,  Robert A. Buckwald ,  Nissim Ben-Yosef

IPC: C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G06K9/00 ,  G06K9/76 ,  C12M1/34 ,  F21V9/08 ,  G02B21/16

CPC classification number: G01N21/6458 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G06K9/00127 ,  G06K9/76 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417

Abstract: A method and hardware for chromosome classification by decorrelation statistical analysis to provide color (spectral) karyotypes and to detect chromosomal aberrations. The method and hardware employ a set of N decorrelation matched filters for chromosome classification. The N decorrelation matched filters are dedicated for extracting decorrelated spectral data from chromosome samples painted according to a specific experimental protocol. The N decorrelation matched filters being described by: ##EQU1## where V.sub.ik min equals minimum V.sub.ik over all i, and V.sub.ik max equals maximum V.sub.ik over all i, and wherein N is an integer greater than two.

Abstract translation: 通过去相关统计分析进行染色体分类的方法和硬件，以提供颜色（光谱）核型和检测染色体畸变。 该方法和硬件采用一组N个去相关匹配滤波器进行染色体分类。 N去相关匹配滤波器专用于从根据特定实验方案绘制的染色体样品中提取去相关光谱数据。 N个去相关匹配滤波器由以下描述：其中Vik min等于所有i上的最小Vik，Vik max等于所有i上的最大Vik，并且其中N是大于2的整数。

公开(公告)号：US5784162A

公开(公告)日：1998-07-21

申请号：US571047

申请日：1995-12-12

Applicant: Dario Cabib ,  Robert A. Buckwald ,  Zvi Malik ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen

Inventor： Dario Cabib ,  Robert A. Buckwald ,  Zvi Malik ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen

IPC: G01B11/00 ,  A61B3/00 ,  A61B3/12 ,  A61B5/00 ,  A61B10/00 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/443 ,  G01J3/45 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/36 ,  G06K9/00 ,  G06K9/76 ,  G01B9/02

CPC classification number: G01N21/6458 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6423

Abstract: According to the present invention there are provided spectral imaging methods for biological research, medical diagnostics and therapy comprising the steps of (a) preparing a sample to be spectrally imaged; (b) viewing the sample through an optical device, the optical device being optically connected to an imaging spectrometer, the optical device and the imaging spectrometer obtaining a spectrum of each pixel of the sample by: (i) collecting incident light simultaneously from all pixels of the sample using collimating optics; (ii) passing the incident collimated light through an interferometer system having a number of elements, to form an exiting light beam; (iii) passing the exiting light beam through a focusing optical system which focuses the exiting light beam on a detector having a two-dimensional array of detector elements, so that at each instant each of the detector elements is the image of one pixel of the sample, so that the real image of the sample is stationary on the plane of the detector array, and so that each of the detector elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths, wherein the linear combination is a function of the instantaneous optical path difference; (iv) rotating one or more of the elements of the interferometer system, so that the optical path difference between the two coherent beams generated by the interferometer system is scanned simultaneously for all the pixels of the sample; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.

Abstract translation: 根据本发明，提供了用于生物学研究，医学诊断和治疗的光谱成像方法，其包括以下步骤：（a）制备待成像的样品; （b）通过光学装置观察样品，光学装置光学连接到成像光谱仪，光学装置和成像光谱仪通过以下步骤获得样品的每个像素的光谱：（i）从所有像素同时收集入射光 的样品使用准直光学; （ii）使入射的准直光通过具有多个元件的干涉仪系统，以形成出射的光束; （iii）使出射的光束通过聚焦光学系统，该聚焦光学系统将出射的光束聚焦在具有检测器元件的二维阵列的检测器上，使得在每个瞬间，每个检测器元件是 样本，使得样本的真实图像在检测器阵列的平面上是静止的，并且使得每个检测器元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合，其中 线性组合是瞬时光程差的函数; （iv）旋转干涉仪系统的一个或多个元件，使得由干涉仪系统产生的两个相干光束之间的光程差同时扫描样品的所有像素; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

公开(公告)号：US5606413A

公开(公告)日：1997-02-25

申请号：US374055

申请日：1995-01-19

Applicant: Peter A. Bellus ,  Terry L. McKinney

Inventor： Peter A. Bellus ,  Terry L. McKinney

IPC: G01J3/12 ,  G01J3/28 ,  G01N21/31 ,  G01N21/35

CPC classification number: G01J3/1256 ,  G01J3/2823

Abstract: To dramatically reduce image data processing requirements in spectroscopic imaging systems, an optical filter is alternatingly tuned to a pair of selected passband wavelengths related to an absorption wavelength of a sample under test, such that only light of the two selected wavelengths received from the test sample are recorded as alternating image frames by a CCD optical detector. Successive pairs of consecutive image frames are computer processed, on a corresponding pixel-by-pixel basis, to generate a series of composite image frames that may be displayed in enhanced contrast to permit real time analysis of a sample characteristic of interest.

Abstract translation: 为了显着降低分光成像系统中的图像数据处理要求，将滤光器交替地调谐到与待测试样品的吸收波长相关的一对选定的通带波长，使得仅从测试样品接收到的两个选定波长的光 通过CCD光学检测器记录为交替图像帧。 连续成对的连续图像帧在相应逐个像素的基础上被计算机处理，以产生可以以增强的对比度显示的一系列合成图像帧，以允许感兴趣的样本特征的实时分析。

公开(公告)号：US5599717A

公开(公告)日：1997-02-04

申请号：US300202

申请日：1994-09-02

Applicant: Tuan Vo-Dinh

Inventor： Tuan Vo-Dinh

IPC: A61B5/00 ,  G01J3/12 ,  G01N21/64

CPC classification number: G01N21/6402 ,  A61B5/0059 ,  G01J3/1256 ,  G01N21/6456 ,  G01N21/6486

Abstract: A method and apparatus for determining the condition of tissue or otherwise making chemical identifications includes exposing the sample to a light source, and using a synchronous luminescence system to produce a spectrum that can be analyzed for tissue condition.

Abstract translation: 用于确定组织状况或以其他方式进行化学鉴定的方法和装置包括将样品暴露于光源，并使用同步发光系统产生可分析组织状况的光谱。

公开(公告)号：US5541413A

公开(公告)日：1996-07-30

申请号：US307734

申请日：1994-09-26

Applicant: Lee H. Pearson ,  Kendall B. Johnson ,  Timothy E. Doyle

Inventor： Lee H. Pearson ,  Kendall B. Johnson ,  Timothy E. Doyle

IPC: G01B11/30 ,  F02K9/96 ,  G01B11/06 ,  G01J3/12 ,  G01N21/17 ,  G01N21/21 ,  G01N21/31 ,  G01N21/59 ,  G01N21/64 ,  G01N21/84 ,  G01N21/88 ,  G01N21/93 ,  G01N21/94 ,  G01N21/35

CPC classification number: G01B11/0625 ,  G01N21/17 ,  G01N21/314 ,  G01N21/6456 ,  G01N21/8806 ,  G01N21/94 ,  G01J3/1256 ,  G01N2021/3133 ,  G01N2021/4709 ,  G01N2021/8427 ,  G01N2021/945 ,  G01N21/21 ,  G01N21/4738 ,  G01N21/474 ,  G01N21/5911 ,  G01N21/8422 ,  G01N21/93

Abstract: A scanning system (10) for inspecting a surface (16) including a light source (30) which generates a beam of light (32) that is reflected, scattered or causes fluorescence at the surface to be inspected. An optical interface (14) receives the beam of light and directs it along a predetermined path extending to and from the surface. An acousto-optic tunable filter (34) tuned to pass light having a wavelength corresponding to a known optical property of a predetermined material is positioned within the path of light. A detector (42) is positioned to receive light emanating from the surface and is configured to monitor the intensity of light at each predetermined wavelength being monitored and generate a corrsponding signal. The system is preferably attached to a scan board (90) thereby enabling the system to be used in scanning a surface. The system also includes a signal processor (22) which processes the signal generated by the detector. The resulting data is displayed by an output device (26).

Abstract translation: PCT No.PCT / US93 / 03831 Sec。 371日期：1994年9月26日 102（e）1994年9月26日PCT 1993年4月23日PCT公布。 公开号WO93 / 22655 日期：1993年11月11日一种用于检查包括产生在被检测表面上被反射，散射或引起荧光的光束（32）的光源（30）的表面（16）的扫描系统（10）。 光学接口（14）接收光束并沿着从表面延伸的预定路径引导光束。 被调谐以通过具有对应于预定材料的已知光学特性的波长的光的声光可调谐滤光器（34）位于光路中。 定位检测器（42）以接收从表面发出的光，并且被配置为监测在被监视的每个预定波长处的光的强度并产生相应的信号。 该系统优选地附接到扫描板（90），从而使得系统能够用于扫描表面。 该系统还包括处理由检测器产生的信号的信号处理器（22）。 所得到的数据由输出设备（26）显示。

公开(公告)号：US5317379A

公开(公告)日：1994-05-31

申请号：US833530

申请日：1992-02-11

Applicant: Frederick M. Ryan ,  James W. Thomson

Inventor： Frederick M. Ryan ,  James W. Thomson

IPC: G01J3/12 ,  G01N21/25 ,  G01J3/06 ,  G01N21/31 ,  G01N21/35

CPC classification number: G01N21/253 ,  G01J3/1256 ,  G01N2201/0826

Abstract: An analyzer measures properties of multiple chemical samples, and includes an optical filter element having a long axis and positioned at a location where simultaneous multiple light beams, corresponding to the chemical samples to be measured, form a diffuse light spot elongated along an axis which is substantially aligned with the filter element long axis. The analyzer also includes a light source, filter means incorporating the filter element for transmitting spectrally selected portions of the light beams, sample cell means for exposing each sample to its associated light beam, and detector means for detecting the light beams after modification by the samples and after transmission by the filter. In a preferred embodiment, optical fibers carry the light beams to and from the chemical samples.

Abstract translation: 一种分析仪测量多个化学样品的性质，并且包括具有长轴并且位于与要测量的化学样品相对应的同时多个光束形成沿轴线延伸的漫射光点的位置处的光学滤光元件， 基本上与过滤元件长轴对齐。 分析仪还包括一个光源，滤光片装置结合有滤光元件，用于透射光谱选择的光束部分，用于将每个样品暴露于其相关联的光束的采样单元装置，以及用于检测由样本修改之后的光束的检测器装置 并通过过滤器传输。 在优选实施例中，光纤将光束传送到化学样品和从化学样品携带光束。

公开(公告)号：US5144374A

公开(公告)日：1992-09-01

申请号：US684041

申请日：1991-04-11

Applicant: Giorgio Grego

Inventor： Giorgio Grego

IPC: G01J3/12 ,  G01J3/00 ,  G01J3/02 ,  G01J3/28 ,  G01J3/44 ,  G01J11/00 ,  G01N21/27 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01J3/02 ,  G01J3/0218 ,  G01M11/338 ,  G01J11/00 ,  G01J2003/1265 ,  G01J3/1256 ,  G01J3/2803 ,  G01J3/2889

Abstract: An optical spectroscopy system, wherein a pulse of a radiation to be analyzed is sent to a diffractive element presenting high temporal chromatic dispersion, and information relevant to the radiation spectrum is obtained from pulse broadening in the time domain.

公开(公告)号：US5131742A

公开(公告)日：1992-07-21

申请号：US597457

申请日：1990-10-12

Applicant: Fred L. Schaff

Inventor： Fred L. Schaff

IPC: G01J3/12 ,  G01J3/28 ,  G01J3/447

CPC classification number: G01J3/447 ,  G01J3/2823 ,  G01J2003/1247 ,  G01J3/0256 ,  G01J3/1256

Abstract: An acousto-optic spectrometer/polarimeter for analyzing an incident broad-band beam including an acousto-optic tunable filter (AOTF) for separating the incident broad-band beam into a diffracted extraordinary narrow-band beam, a diffracted ordinary narrow-band beam, and at least one undiffracted broad-band beam; first, second and third detectors respectively positioned to respectfully receive at least a portion of the diffracted extraordinary narrow-band beam, the diffracted ordinary narrow broad-band beam and the at least one undiffracted broad-band beam, and to respectively provide first, second and third signals based thereon; a subtracter for providing a polarimeter output by taking the difference between the first and the second signals; and an adder for providing a spectrometer output by adding the first and the second signals. The acousto-optic spectrometer/polarimeter according to the present invention allows broad-band imaging, spectroscopy and polarimetry to be performed simultaneously and through the same optical aperture.

Abstract translation: 一种用于分析包括声光可调滤波器（AOTF）的入射宽带束的声光谱仪/偏振计，用于将入射的宽带束分离成衍射的非常窄带束，衍射的普通窄带束， 和至少一个未衍射的宽带束; 第一，第二和第三检测器被分别定位成尊重地接收衍射的非常窄带束，衍射普通窄带宽波束和至少一个未衍射宽带波束的至少一部分，并且分别提供第一，第二，第二 和基于此的第三信号; 减法器，用于通过取第一和第二信号之间的差来提供偏振计输出; 以及用于通过添加第一和第二信号来提供光谱仪输出的加法器。 根据本发明的声光谱仪/偏光计允许同时进行宽带成像，光谱学和偏振测定，并且通过相同的光学孔径。

公开(公告)号：US5120961A

公开(公告)日：1992-06-09

申请号：US494342

申请日：1990-03-16

Applicant: Kenneth H. Levin ,  Frank Y. Li

Inventor： Kenneth H. Levin ,  Frank Y. Li

IPC: G01J1/44 ,  G01J3/02 ,  G01J3/12 ,  G01J3/42

CPC classification number: G01J3/02 ,  G01J3/0218 ,  G01J3/1256 ,  G01J2001/4242 ,  G01J3/42

Abstract: An acousto-optical filter (AOTF) is used in a spectrometer for analysis of samples. The spectrometer provides continuous wave RF excitation through the crystal, and the spectrometer provides control and modulation for said RF source. The signal to noise ratio is improved by use of a lock-in amplifier which demodulates the modulation frequency. Fiber optics are used to connect the crystal to the source, and the source to the detection system. A digital lock-in amplifier is designed which increases the efficiency, accuracy, sensitivity and decreases the cost of conventional analog lock-in amplifier.

Abstract translation: 在光谱仪中使用声光滤波器（AOTF）来分析样品。 光谱仪通过晶体提供连续波RF激发，光谱仪为所述RF源提供控制和调制。 通过使用解调调制频率的锁相放大器来提高信噪比。 光纤用于将晶体连接到源，将源连接到检测系统。 设计了一种数字锁相放大器，可提高传统模拟锁定放大器的效率，精度，灵敏度和降低成本。