公开(公告)号：WO0206407A3

公开(公告)日：2003-02-13

申请号：PCT/US0122455

申请日：2001-07-17

Applicant: HARVARD COLLEGE

Inventor： CHAPMAN ROBERT G ,  OSTUNI EMANUELE ,  LIANG MICHAEL N ,  YAN LIN ,  WHITESIDES GEORGE M

IPC: A61F2/28 ,  A61L15/42 ,  A61L27/34 ,  A61L27/50 ,  A61L31/10 ,  B32B27/06 ,  C08J7/12 ,  B05D1/18

CPC classification number: B82Y30/00 ,  A61L15/42 ,  A61L27/34 ,  A61L27/50 ,  A61L31/10 ,  B32B27/06 ,  B82Y5/00 ,  Y10T428/26 ,  Y10T428/265 ,  Y10T428/268 ,  Y10T428/269 ,  Y10T428/31504 ,  Y10T428/31678 ,  Y10T428/31681 ,  Y10T428/31725 ,  Y10T428/31855

Abstract: The present invention provides articles resistant to the adsorption of proteins, cells and bacteria. The articles can either have a chemical chain bonded thereon where the chemical chain can comprise a terminal group free of a hydrogen bond donor or where a hydrogen bond donor is sufficiently buried such that an exposed surface of the article including the chemical chain is free of a hydrogen bond donor. The chemical chain, or plurality of chemical chains, can comprise a monolayer such as a self-assembled monolayer (SAM) which can be homogeneous (one type of SAM) or mixed, i.e. or more different types of SAMs. Other more specific examples of chemical chains are provided. The plurality of chemical chains can comprise a polymer such as a polyamine. In many aspects, the plurality of chemical chains is sufficiently free of cross-linking or branching. The present invention also provides an article capable of specific binding of a desired biomolecule while preventing non-specific binding of biomolecules.

Abstract translation: 本发明提供对蛋白质，细胞和细菌的吸附有抗性的制品。 制品可以具有键合在其上的化学链，其中化学链可以包含不含氢键供体的末端基团或者氢键供体被充分掩埋，使得包含化学链的制品的暴露表面不含 氢键供体。 化学链或多个化学链可以包括单层，例如可以是均相（一种类型的SAM）或混合的，即或更多不同类型的SAM的自组装单层（SAM）。 提供了化学链的其他更具体的实例。 多个化学链可以包括诸如多胺的聚合物。 在许多方面，多个化学链足够地没有交联或分支。 本发明还提供能够特异性结合所需生物分子同时防止生物分子的非特异性结合的制品。

公开(公告)号：WO0170389A3

公开(公告)日：2002-03-07

申请号：PCT/US0108206

申请日：2001-03-15

Applicant: HARVARD COLLEGE

Inventor： OSTUNI EMANUELE ,  KANE RAVI ,  DUFFY DAVID C ,  JACKMAN REBECCA J ,  WHITESIDES GEORGE M

IPC: G01N33/48 ,  B01J19/00 ,  C12N1/00 ,  C12N11/06 ,  C40B60/14 ,  G01N33/543

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00427 ,  B01J2219/0043 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00743 ,  B82Y5/00 ,  C12M21/08 ,  C12M25/04 ,  C12M25/08 ,  C12M33/00 ,  C12N11/06 ,  C40B60/14

Abstract: The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.

Abstract translation: 本发明提供了一种用于选择性地将电池施加到表面的预定区域的掩模系统。 掩模邻近表面定位以覆盖表面的一些部分，同时允许表面的其它部分保持未被覆盖。 然后将细胞施加到表面的未覆盖部分并除去掩模。 或者，将细胞粘附促进剂施加到表面的未覆盖部分，然后在从表面除去掩模之前或之后将细胞施加到表面。 掩蔽系统可以至少在与细胞接触的那些表面上预先涂覆有细胞粘附抑制剂以抵抗细胞的吸收，从而在去除掩模时避免细胞损伤（如果细胞在 去除面膜）。 可以使用与要图案化的表面粘合保形接触的聚合物弹性体掩模。

公开(公告)号：WO0203142A2

公开(公告)日：2002-01-10

申请号：PCT/US0121151

申请日：2001-07-02

Applicant: HARVARD COLLEGE ,  JACOBS HEIKO O ,  WHITESIDES GEORGE M

Inventor： JACOBS HEIKO O ,  WHITESIDES GEORGE M

IPC: G03F7/00 ,  G03G5/02

CPC classification number: G03G5/02 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  G03F7/0002

Abstract: A method and apparatus for providing electric microcontact printing is provided. A stamp is brought into contact with the surface of a substrate to provide high resolution features. Aspects of the invention may be used for data storage, microcontact printing, and for other applications requiring high resolution pattern transfer.

Abstract translation: 提供了一种用于提供电微接触印刷的方法和装置。 使印模与基板的表面接触以提供高分辨率特征。 本发明的各方面可用于数据存储，微接触印刷以及需要高分辨率图案转印的其它应用。

公开(公告)号：WO2017014828A9

公开(公告)日：2017-02-16

申请号：PCT/US2016032706

申请日：2016-05-16

Applicant: HARVARD COLLEGE ,  UNIV SOUTHAMPTON

Inventor： TSALOGLOU MARIA-NEFELI ,  NEMIROSKI ALEX ,  CHRISTODULEAS DIONYSIOS ,  CAMCI-UNAL GULDEN ,  CONNELLY JOHN T ,  FERNANDEZ-ABEDUL MARIA TERESA ,  WHITESIDES GEORGE M

IPC: C12Q1/68 ,  G01N27/26

CPC classification number: C12Q1/68 ,  B01L3/5023 ,  B01L3/5027 ,  B01L7/00 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2300/1827 ,  G01N33/543

Abstract: A microfluidic, electrochemical device for detecting a genetic material is described, including: one or more cellulosic layers comprising at least one of a hydrophilic test zone and a hydrophilic sample deposition zone in fluid communication with each other; one or more amplification agents selected for amplifying a genetic material and embedded in the hydrophilic test zone or the sample deposition zone; one or more binding agents embedded in the test zone or the sample deposition zone and selected for binding the amplified genetic material to result in a change of the concentration of a signaling chemical, wherein the signaling chemical is either embedded in the test zone or the sample deposition zone prior to the binding or is a newly generated product of the binding; and an electrode assembly comprising one or more electrodes in fluidic contact with the test zone and configured to interact with the signaling chemical to result in a current change readable by an electrochemical reader. Methods of using the device and kit containing the device are also described.

Abstract translation: 描述了用于检测遗传物质的微流体电化学装置，包括：一个或多个纤维素层，其包括彼此流体连通的亲水性测试区和亲水性样品沉积区中的至少一个; 选择一种或多种扩增剂，用于扩增遗传物质并嵌入亲水测试区或样品沉积区中; 一种或多种嵌入测试区或样品沉积区中的结合剂，并选择用于结合扩增的遗传物质以导致信号化学物质浓度的变化，其中信号化学物质嵌入测试区或样品中 沉积区，或者是新产生的结合产物; 以及包括一个或多个电极的电极组件，所述一个或多个电极与所述测试区流体接触并且被配置为与所述信号化学物质相互作用以导致电化学读取器可读的电流变化。 还描述了使用装置和包含该装置的套件的方法。

公开(公告)号：WO2016167851A3

公开(公告)日：2016-12-22

申请号：PCT/US2016013013

申请日：2016-01-12

Applicant: HARVARD COLLEGE

Inventor： LESSING JOSHUA AARON ,  WHITESIDES GEORGE M ,  SHEVCHENKO YANINA ,  MOSADEGH BOBAK ,  GALLOWAY KEVIN C ,  TAYI ALOK SURYAVAMSEE

IPC: B25J19/02

CPC classification number: G01T7/00 ,  A61F2002/7615

Abstract: A soft robotic device with a variety of sensors and/or imaging areas is described. The sensor and/or imaging area may be embedded in the soft body or the strain limiting layer of the soft robotic device, attached to the soft body or the strain limiting layer of the soft robotic device, or otherwise linked to the soft body or the strain limiting layer of the soft robotic device.

Abstract translation: 描述了具有各种传感器和/或成像区域的软的机器人装置。 传感器和/或成像区域可以嵌入软的机器人装置的软体或应变限制层，附着到软的机器人装置的软体或应变限制层，或以其他方式连接到软体或 软机器人装置的应变限制层。

公开(公告)号：WO2013148340A3

公开(公告)日：2013-12-05

申请号：PCT/US2013032297

申请日：2013-03-15

Applicant: HARVARD COLLEGE

Inventor： MORIN STEPHEN A ,  SHEPHERD ROBERT F ,  STOKES ADAM ,  ILIEVSKI FILIP ,  MARTINEZ RAMSES V ,  BRANCH JAMIE L ,  FISH CARINA R ,  JIN LIHUA ,  NUNES RUI M D ,  SUO ZHIGANG ,  WHITESIDES GEORGE M

IPC: B25J9/10 ,  B25J9/14

CPC classification number: B25J9/142 ,  A47L9/2836 ,  B25J9/1075 ,  B25J9/1697 ,  Y10S901/22 ,  Y10T74/20305

Abstract: Systems and methods for providing flexible robotic actuators are disclosed. Thanks to the concentrical positioning of a plurality of inflatable channels, the claimed soft robot is capable of providing a radial deflection motion. A method for operating the disclosed robotic systems is also disclosed.

Abstract translation: 公开了用于提供灵活的机器人致动器的系统和方法。 由于多个可充气通道的同心定位，所要求保护的软式机器人能够提供径向偏转运动。 还公开了用于操作所公开的机器人系统的方法。

公开(公告)号：WO2013130760A3

公开(公告)日：2013-11-14

申请号：PCT/US2013028250

申请日：2013-02-28

Applicant: HARVARD COLLEGE

Inventor： MARTINEZ RAMSES V ,  WHITESIDES GEORGE M

IPC: F15B15/10

CPC classification number: F15B15/103 ,  B25J9/142 ,  F15B2215/305 ,  F21V1/12 ,  F21V17/02 ,  Y10T29/4998

Abstract: Soft pneumatic actuators based on composites consisting of elastomers (130) with embedded sheet or fiber structures, e.g., paper or fabric, (149) that are flexible but not extensible are described. On pneumatic inflation, these actuators move anisotropically, based on the motions accessible by their composite structures. They are inexpensive, simple to fabricate, light in weight, and easy to actuate. This class of structure is versatile: the same principles of design lead to actuators that respond to pressurization with a wide range of motions (bending, extension, contraction, twisting, and others). Paper, when used to introduce anisotropy into elastomers, can be readily folded into three-dimensional structures following the principles of origami; these folded structures increase the stiffness and anisotropy of the elastomeric actuators, while keeping them light in weight.

Abstract translation: 描述了基于由具有柔性但不可伸长的嵌入片或纤维结构的弹性体（130），例如纸或织物（149）组成的软气动执行器。 在气动膨胀时，这些致动器基于其复合结构可触及的运动而各向异性地移动。 它们便宜，制造简单，重量轻，易于起动。 这种结构类型是通用的：相同的设计原理导致执行器响应于各种运动（弯曲，延伸，收缩，扭转等）的加压。 当用于将各向异性引入弹性体时，纸可以容易地折叠成按折纸原理的三维结构; 这些折叠的结构增加了弹性致动器的刚性和各向异性，同时保持其重量轻。

公开(公告)号：WO2009121041A3

公开(公告)日：2009-12-17

申请号：PCT/US2009038699

申请日：2009-03-27

Applicant: HARVARD COLLEGE ,  SIEGEL ADAM C ,  PHILLIPS SCOTT T ,  DICKEY MICHAEL D ,  ROZKIEWICZ DOROTA ,  WILEY BENJAMIN ,  WHITESIDES GEORGE M ,  MARTINEZ ANDRES W

Inventor： SIEGEL ADAM C ,  PHILLIPS SCOTT T ,  DICKEY MICHAEL D ,  ROZKIEWICZ DOROTA ,  WILEY BENJAMIN ,  WHITESIDES GEORGE M ,  MARTINEZ ANDRES W

IPC: G01N35/00 ,  G01N27/26 ,  G01N33/48

CPC classification number: B01L3/502707 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/025 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2300/0819 ,  B01L2300/087 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2300/161 ,  B01L2300/1827 ,  B01L2400/0406 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention features an assay device, a microfluidic device, and a method of detecting the presence of high electrolyte concentration in a fluid sample. The assay device comprises a porous, hydrophilic substrate; a fluid-impermeable barrier defining a boundary of an assay region and a boundary of a main channel region, the main channel region fluidically connected to the assay region; and a strip of conductive material disposed on the porous, hydrophilic substrate. The microfluidic device comprises a porous, hydrophilic substrate; a fluid-impermeable barrier, the barrier permeating the thickness of the porous, hydrophilic substrate and defining within the porous, hydrophilic substrate a boundary of an open-ended channel having first and second lateral walls; and an electrically conductive pathway disposed on the porous, hydrophilic substrate, the electrically conductive pathway comprising (i) a strip of conductive material forming an open circuit in the absence of an electrically conductive material bridging the first and second lateral walls; and (ii) a battery, an electrically-responsive indicator, and a resistor electrically connected to the strip of conductive material.

Abstract translation: 本发明的特征在于测定装置，微流体装置和检测流体样品中高电解质浓度的存在的方法。 测定装置包括多孔亲水底物; 限定测定区域和主通道区域边界的流体不渗透屏障，主通道区域与流体连接到测定区域的主通道区域; 以及设置在多孔亲水基底上的导电材料条。 微流体装置包括多孔的亲水基底; 流体不可渗透的屏障，所述屏障渗透所述多孔亲水基底的厚度，并且在所述多孔亲水基底内限定具有第一和第二侧壁的开放式通道的边界; 以及设置在所述多孔亲水基底上的导电通路，所述导电路径包括（i）在桥接所述第一和第二侧壁的导电材料不存在的情况下形成开路的导电材料条; 以及（ii）电池，电响应指示器和电连接到导电材料条的电阻器。

公开(公告)号：WO2007143121A3

公开(公告)日：2008-01-24

申请号：PCT/US2007012989

申请日：2007-06-01

Applicant: HARVARD COLLEGE ,  KRISHNAMURTHY VIJAY M ,  ESTROFF LARA A ,  SEMETEY VINCENT ,  THOMAS III SAMUEL WILLIAM ,  KAUFMAN GEORGE KARL ,  BILGICER ZIHNI BASAR ,  WHITESIDES GEORGE M

Inventor： KRISHNAMURTHY VIJAY M ,  ESTROFF LARA A ,  SEMETEY VINCENT ,  THOMAS III SAMUEL WILLIAM ,  KAUFMAN GEORGE KARL ,  BILGICER ZIHNI BASAR ,  WHITESIDES GEORGE M

IPC: C07K16/06

CPC classification number: C07K16/065 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/54 ,  Y10S436/822

Abstract: The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment.

Abstract translation: 本发明公开了一种纯化二价抗体或抗体片段的方法，所述二价抗体或抗体片段在使用非色谱法的抗体或抗体片段来源的两个Fab位点都是有活性的，其包括通过将多价半抗原加入到 可溶性抗体或抗体片段的盐溶液，其中多价半抗原具有两个半抗原之间的接头，其有效地防止配体的半抗原与相同的抗体或抗体片段的结合。

公开(公告)号：WO2006083833A3

公开(公告)日：2007-02-22

申请号：PCT/US2006003354

申请日：2006-01-31

Applicant: HARVARD COLLEGE ,  WEIBEL DOUGLAS B ,  LEE ANDREW ,  POTENTA SCOTT ,  SIEGEL ADAM ,  KRUITHOF MAARTEN ,  WHITESIDES GEORGE M

Inventor： WEIBEL DOUGLAS B ,  LEE ANDREW ,  POTENTA SCOTT ,  SIEGEL ADAM ,  KRUITHOF MAARTEN ,  WHITESIDES GEORGE M

IPC: F16K3/24

CPC classification number: F15B1/04 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0636 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/082 ,  F15B2201/21 ,  F15B2201/305 ,  F15B2201/4053 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0034 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/0396 ,  Y10T137/2213 ,  Y10T137/4824 ,  Y10T137/6161

Abstract: A microfluidic valve assembly includes a structure defining a microfluidic fluid path and an actuator that can be moved between different positions controlling flow through the channel. In one embodiment, the actuator can be threaded into at least a portion of the structure, and can be moved rotationally between a first position, causing relatively greater constriction of a microfluidic fluid path, and a second position causing relatively lesser constriction of the fluid path. Actuating the actuator, e.g., by rotation, can deform material between the valve and the fluid path, thereby constricting at least a portion of the underlying fluid path and regulating the flow of a fluid in the fluid path. In another aspect, the invention provides a reservoir into which fluid can be placed and from which fluid can be introduced into a microfluidic system. In one embodiment, the reservoir is expandable and thereby able to store fluid under pressure for delivery to a microfluidic system.

Abstract translation: 微流体阀组件包括限定微流体流体路径的结构和可以在控制流过通道的不同位置之间移动的致动器。 在一个实施例中，致动器可以被螺纹连接到结构的至少一部分中，并且可以在第一位置和第二位置之间旋转地移动，第一位置引起微流体流体路径的相对较大的收缩，并且引起相对较小的流体路径收缩的第二位置 。 例如通过旋转来致动致动器可以在阀和流体路径之间变形材料，从而收缩下面的流体路径的至少一部分并且调节流体路径中的流体的流动。 在另一方面，本发明提供了一种储存器，流体可以放置在该储存器中，并且可以从该储存器将流体引入到微流体系统中。 在一个实施例中，储存器是可膨胀的并且因此能够在压力下存储流体以递送到微流体系统。