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公开(公告)号：KR1020120106911A
公开(公告)日：2012-09-27
申请号：KR1020110023509
申请日：2011-03-16
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for establishing a cell surface expression system using an autotransporter is provided to enable secretion of target proteins. CONSTITUTION: A surface expression vector contains a signal sequence, a passenger domain, a linker site, and a cloning site in which the passenger domain site is deleted from autotransporter proteins of bacteria. A vector contains TEV protease cleavage site inserted to the cloning site. The autotransporter protein has an amino acid sequence of sequence number 1. The passenger domain site has a sequence of 29th to 785th residue in an amino acid of sequence number 1. [Reference numerals] (AA) Cleavable leader sequence; (BB) Hypothetic linker site; (CC) Passenger domain with adhering function; (DD) Pertacin-like domain; (EE) Autotransporter series; (FF) N-terminal passenger domain; (GG) C-terminal passenger domain; (HH) Removal site
Abstract translation: 目的：提供使用自身转运蛋白建立细胞表面表达系统的方法，以使靶蛋白分泌。 构成：表面表达载体含有信号序列，乘客结构域，接头位点和克隆位点，其中乘客域位点从细菌的自身转运蛋白中缺失。 载体含有插入克隆位点的TEV蛋白酶切割位点。 自身转运蛋白具有序列号1的氨基酸序列。载体结构域位点在序列号1的氨基酸中具有29至785位残基的序列。[参考数字]（AA）可剪切前导序列; （BB）虚拟连接位点; （CC）具有粘性功能的客运区; （DD）Pertacin样结构域; （EE）自动输送机系列; （FF）N端乘客域; （GG）C终端乘客区域; （HH）搬迁站点
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公开(公告)号：KR1020100130674A
公开(公告)日：2010-12-14
申请号：KR1020090049309
申请日：2009-06-04
Applicant: 고려대학교 산학협력단
CPC classification number: A61K36/53 , A23V2200/328 , A61K2236/331 , Y10S514/866
Abstract: PURPOSE: A composition containing lemon balm essential oil for reducing blood glucose is provided to improve GCK(hepatic gludokinase) activity and to suppress G6Pase(glucose-6-phosphatase) and PEPCK(phosphoenolpyruvate carboxykinase) activity. CONSTITUTION: A composition for reducing blood glucose contains lemon balm essential oil as an active ingredient. The lemon balm essential oil is isolated by steam distillation. A pharmaceutical composition and health food for preventing and treating diabetes contain the composition for removing blood glucose as an active ingredient.
Abstract translation: 目的：提供含有降低血糖的柠檬香精油的组合物，以改善GCK（肝纤维蛋白酶）活性，抑制G6Pase（葡萄糖-6-磷酸酶）和PEPCK（磷酸烯醇丙酮酸羧激酶）活性。 构成：用于降低血糖的组合物含有柠檬香精油作为活性成分。 柠檬香精油通过蒸汽蒸馏分离。 用于预防和治疗糖尿病的药物组合物和保健食品含有除去作为有效成分的血糖的组合物。
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公开(公告)号：KR100894272B1
公开(公告)日：2009-04-21
申请号：KR1020070082816
申请日：2007-08-17
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 본 발명은 초임계 이산화탄소를 이용한 식육의 연도증진방법에 관한 것으로, 더욱 구체적으로 식육의 연도를 증진하는 방법에 있어서, 초임계 이산화탄소를 식육에 처리하는 단계를 포함하는 것을 특징으로 하는 식육의 연도증진방법에 관한 것이다.
본 발명에 따르면, 종래 고압의 기체를 이용하는 물리적 방법에 의한 연도증진 방법에 비하여, 본 발명에서 새롭게 선정한 초임계 이산화탄소의 연도증진방법은 식육에 이산화탄소의 초임계점인 73 atm이상으로 31.1℃에서 10분 동안 초임계 이산화탄소를 처리함으로써, 처리하지 않은 식육에 비해 뚜렷한 연육작용 효과를 얻을 수 있음을 확인하였다. 따라서 이러한 효과는 고품질의 가공 육류 제조에 사용될 수 있을 것으로 기대된다.
초임계 이산화탄소, 초임계점, 식육, 양념돈육, 연도-
公开(公告)号：KR100843743B1
公开(公告)日：2008-07-04
申请号：KR1020070032108
申请日：2007-03-31
Applicant: 고려대학교 산학협력단
Abstract: A method of extracting soybean oil using supercritical fluid is provided to maximally extract active material such as lecithin, isoflavone and the like and not to leave an organic solvent. Ground soybeans are put into a reactor of 35 to 80deg.C, carbon dioxide gas is introduced into the reactor through a conduit connected to the reactor after closing the reactor and the reactor is then pressurized to 80 to 300bar to produce supercritical fluid from the carbon dioxide. The reactor is maintained at the above temperature and pressure for 6 to 9hr to extract fat in a first step. After removing the supercritical fluid, 5 to 15 mole fraction of ethanol, based on the supercritical fluid and carbon dioxide are put into the reactor and the reactor is then pressurized to 80 to 300bar at 35 to 80deg.C to extract phospholipid using supercritical fluid in a second step.
Abstract translation: 提供使用超临界流体提取大豆油的方法以最大限度地提取活性物质如卵磷脂，异黄酮等并且不留下有机溶剂。 将研磨的大豆放入35至80℃的反应器中，在关闭反应器之后，通过连接到反应器的导管将二氧化碳气体引入反应器中，然后将反应器加压至80至300巴，以从碳中产生超临界流体 二氧化碳。 在第一步骤中将反应器保持在上述温度和压力下6至9小时以提取脂肪。 除去超临界流体后，将基于超临界流体和二氧化碳的5至15摩尔份量的乙醇放入反应器中，然后将反应器在35至80℃下加压至80至300巴，以使用超临界流体萃取磷脂 第二步。
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