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    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME
    11.
    发明申请
    DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 审中-公开
    DNA序列方法和检测器及其执行系统

    公开(公告)号：WO2011082419A3

    公开(公告)日：2012-02-09

    申请号：PCT/US2011020117

    申请日：2011-01-04

    Applicant: LIFE TECHNOLOGIES CORP ,  SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    Inventor： SUN HONGYE ,  NORDMAN ERIC S ,  OLDHAM MARK F ,  O'NEILL JOHN R ,  CONNELL CHARLES ,  ULMANELLA UMBERTO ,  LAU ALDRICH N K ,  KOTSEROGLOU THEOFILOS ,  LIVAK KENNETH J

    IPC: C12Q1/68 ,  G01N27/414 ,  G01N33/50

    CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    Abstract translation: 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极装置和设置在纳米通道中的多个纳米器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔也是制备它们的方法。 在一些实施方案中，提供用于减缓单链DNA（ssDNA）穿过纳米孔的易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

    METHODS OF ANALYZING BINDING INTERACTIONS
    12.
    发明申请
    METHODS OF ANALYZING BINDING INTERACTIONS 审中-公开
    分析结合相互作用的方法

    公开(公告)号：WO2008005674A3

    公开(公告)日：2008-12-24

    申请号：PCT/US2007071270

    申请日：2007-06-14

    Applicant: APPLERA CORP ,  SUN HONGYE ,  LIVAK KENNETH J

    Inventor： SUN HONGYE ,  LIVAK KENNETH J

    IPC: G01N33/53

    CPC classification number: G01N33/566 ,  G01N33/48721

    Abstract: The present disclosure relates to methods of analyzing binding interactions between a binding component and a receptor component by translocating unbound and any bound components through a pore and detecting the unbound and bound components.

    Abstract translation: 本公开内容涉及通过将未结合的组分和任何结合的组分通过孔移位并检测未结合和结合的组分来分析结合组分和受体组分之间的结合相互作用的方法。

    ENCODING AND DECODING REACTIONS FOR DETERMINING TARGET MOLECULES
    19.
    发明申请
    ENCODING AND DECODING REACTIONS FOR DETERMINING TARGET MOLECULES 审中-公开
    编码和解码反应用于确定目标分子

    公开(公告)号：WO2005098040A2

    公开(公告)日：2005-10-20

    申请号：PCT/US2005/010184

    申请日：2005-03-24

    Applicant: APPLERA CORPORATION ,  ANDERSEN, Mark R. ,  LIVAK, Kenneth J. ,  BROOMER, Adam ,  CHEN, Caifu

    Inventor： ANDERSEN, Mark R. ,  LIVAK, Kenneth J. ,  BROOMER, Adam ,  CHEN, Caifu

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2525/191 ,  C12Q2525/185 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2521/313 ,  C12Q2537/143 ,  C12Q2525/161

    Abstract: The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.

    Abstract translation: 本发明涉及用于使用编码和解码反应检测至少一个样品中靶多核苷酸序列和蛋白质的存在或不存在（或定量）的方法，试剂和试剂盒。 当特定靶多核苷酸例如存在于样品中时，在包含可寻址引物部分的编码反应中形成反应产物。 可以在解码扩增反应中使用至少一个标记探针和至少一个地址引物，从而根据序列是否存在提供可检测的信号值。 在一些实施方案中，编码包含与接头探针的连接反应，并分析单核苷酸多态性（SNP）。

    COMBINATORIAL NUCLEOBASE OLIGOMERS COMPRISING UNIVERSAL BASE ANALOGUES AND METHODS FOR MAKING AND USING SAME
    20.
    发明申请
    COMBINATORIAL NUCLEOBASE OLIGOMERS COMPRISING UNIVERSAL BASE ANALOGUES AND METHODS FOR MAKING AND USING SAME 审中-公开
    包含通用基础模拟物的组合核酸寡聚体及其制备和使用方法

    公开(公告)号：WO2004111072A3

    公开(公告)日：2005-06-30

    申请号：PCT/US2004018800

    申请日：2004-06-10

    Applicant: APPLERA CORP ,  LIVAK KENNETH J ,  MULLAH K BASHAR

    Inventor： LIVAK KENNETH J ,  MULLAH K BASHAR

    IPC: B01J19/00 ,  C07H21/00 ,  C07H21/04 ,  C07K1/04 ,  C07K14/00 ,  C12N15/11 ,  C12P19/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B50/00

    CPC classification number: C12Q1/6811 ,  C07H21/00 ,  C07H21/04 ,  C12Q2525/197 ,  C12Q2525/117

    Abstract: The invention relates to insulating combinatorial nucleobase oligomers that comprise universal base analogs, where the oligomers are formed by the ligation of two or more oligomer "blocks" via a covalent linkage. Universal bases may serve to insulate specifically binding nucleobases from the effects of the covalent linker region joining two oligomer blocks together, so that the universal bases at least partially negate the Tm penalty caused by the covalent linkage, effective to reduce the required minimal length of the oligomer blocks and the combinatorial oligomer. The resulting insulating nucleobase combinatorial oligomers find use in any hybridization-based application, including use as probes and primers. The combinatorial oligomers of the present invention provide advantages over existing combinatorial oligomer systems currently known in the art.

    Abstract translation: 本发明涉及包含通用碱基类似物的绝缘组合核碱基寡聚体，其中通过共价键连接两个或多个低聚物“嵌段”形成低聚物。 通用碱基可以用于将特异性结合的核碱基与连接两个低聚物嵌段的共价连接体区域的作用绝缘，使得通用碱基至少部分地消除由共价键引起的Tm损失，有效地减少所需的最小长度 低聚物嵌段和组合低聚物。 所得到的绝缘核碱基组合寡聚体可用于任何基于杂交的应用，包括用作探针和引物。 本发明的组合寡聚体提供优于现有技术中已知的组合式低聚物体系的优点。

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