Abstract:
A COXIII gene derived from Sancheong berkshire and a primer specific to the Sancheong berkshire designed from the COXIII gene are provided to identify the Sancheong berkshire accurately and conveniently within a short time and be used to develop a novel Korean min-pig based on the Sancheong berkshire. A COXIII gene derived from Sancheong berkshire consists of SEQ ID: NO. 1. A primer for identifying the Sancheong berkshire consists of SEQ ID : NO. 3 or 4. A method for identifying the Sancheong berkshire comprises the steps of: (a) obtaining cDNA or gDNA from a pig; (b) subjecting the obtained cDNA or gDNA as a template to PCR using a primer pair consisting of SEQ ID : NOs. 3 and 4; and (c) electrophoresing a reaction product obtained from the step(b) to identify a band specific to the Sancheong berkshire.
Abstract translation:提供了一种来自Sancheong berkshire的COXIII基因,以及由COXIII基因设计的针对Sancheong berkshire的引物,可在短时间内准确,方便地鉴定Sancheong berkshire,并用于开发基于Sancheong berkshire的小型韩国小猪 。 源自Sancheong berkshire的COXIII基因由SEQ ID NO: 用于鉴定Sancheong berkshire的引物由SEQ ID NO: 3.一种鉴定三清伯克郡的方法,包括以下步骤:(a)从猪获得cDNA或gDNA; (b)使用由SEQ ID NO:构成的引物对,使得到的cDNA或gDNA作为模板进行PCR。 3和4; 和(c)对从步骤(b)获得的反应产物进行电泳以鉴定针对Sancheong Berkshire的特异性条带。
Abstract:
PURPOSE: A novel SNP marker for early selecting pigs with preferable parts of high content and a method using the same are provided to early select breeding pigs. CONSTITUTION: A method for evaluating economic characters of pigs comprises a step of selecting a pig that SNP locus of serial number 3 is CT, SNP locus of serial number 13 is CT, SNP locus of serial number 15 is AA or GG, SNP locus of serial number 16 is GG, or SNP locus of serial number 17 is GG; and a step of determining the pig which has high quality as one which corresponds to many loci.
Abstract:
A cDNA chip which early diagnoses a gene related to the growth, meat quality and breeding character of pig is provided to analyze the massive gene source of pig at once. A method for producing a cDNA chip which diagnoses a gene related to the growth, meat quality and breeding character of pig comprises: a step of preparing tissue sample from a tissue and blood of pig; a step of isolating RNA from the tissue sample; a step of producing cDNA library with 4,172 ESTs cloned from RNA; a step of producing a 4,200 cDNA chips containing 28 gene of beta-actin(Acc. No. DQ845171) and 18S(Acc. No. AF102857); and a step of mixing 254 gene having the significance in growth, meat quality and breeding character and 10 positive control genes to produce 264 cDNA chips.
berkshire ) 유래의 신규한 COXⅢ 유전자, 이로부터 디자인된 산청흑돼지 감별용 프라이머 및 이 프라이머를 이용한 산청흑돼지 감별 방법에 관한 것이다. 본 발명의 산청흑돼지 유래의 신규한 COXⅢ 유전자 및 이로부터 디자인된 산청흑돼지 특이적 프라이머를 이용한 산청흑돼지 감별 방법은 정확하고 간편할 뿐만 아니라, 단시간 내에 감별할 수 있는 효과가 있으며, 나아가, 산청흑돼지를 토대로 한국형 미니돼지 신품종 창출에 따른 계통화와 그 혈통의 보존에 사용될 수 있을 뿐만 아니라, 본 발명의 방법을 응용하면 돼지뿐만 아니라, 우리나라에서 사육되는 여러 종류의 가축들에 대한 순수혈통 고정 및 계통화를 통해 생산된 특산물을 확인할 때도 사용되어 질 수 있어 농축산업의 분야에 있어서 매우 유용할 것이다. 산청흑돼지, COXⅢ, PCR