돼지의 조기선발을 위한 신규한 마커와 이를 이용한 평가방법
    1.
    发明公开
    돼지의 조기선발을 위한 신규한 마커와 이를 이용한 평가방법 有权
    SNP标记和评估猪肉的方法

    公开(公告)号:KR1020120127178A

    公开(公告)日:2012-11-21

    申请号:KR1020110141381

    申请日:2011-12-23

    CPC classification number: C12Q1/6827 C12Q1/6888 C12Q2600/124 C12Q2600/156

    Abstract: PURPOSE: A novel SNP marker for early selecting pigs with preferable parts of high content and a method using the same are provided to early select breeding pigs. CONSTITUTION: A method for evaluating economic characters of pigs comprises a step of selecting a pig that SNP locus of serial number 3 is CT, SNP locus of serial number 13 is CT, SNP locus of serial number 15 is AA or GG, SNP locus of serial number 16 is GG, or SNP locus of serial number 17 is GG; and a step of determining the pig which has high quality as one which corresponds to many loci.

    Abstract translation: 目的:提供早期选择高含量部分猪的新型SNP标记物及其使用方法,以提早选择育种猪。 构成:评价猪经济特性的方法包括选择序列号3为SNP SNP为CT的猪,序列号13为SNP的SNP为CT,序列号15的SNP位点为AA或GG,SNP位点为 序列号16是GG,序列号17的SNP位点是GG; 并且将具有高质量的猪确定为对应于许多基因座的步骤。

    돼지의 성장, 육질 및 번식 형질과 관련된 유전자를 조기에진단할 수 있는 cDNA chip 과 그 개발방법 및상기 칩을 이용한 성장, 육질 및 번식 형질이 우수한 종돈선별방법

    公开(公告)号:KR1020090065412A

    公开(公告)日:2009-06-22

    申请号:KR1020080046982

    申请日:2008-05-21

    CPC classification number: C12Q1/6888 C12Q2600/124

    Abstract: A cDNA chip which early diagnoses a gene related to the growth, meat quality and breeding character of pig is provided to analyze the massive gene source of pig at once. A method for producing a cDNA chip which diagnoses a gene related to the growth, meat quality and breeding character of pig comprises: a step of preparing tissue sample from a tissue and blood of pig; a step of isolating RNA from the tissue sample; a step of producing cDNA library with 4,172 ESTs cloned from RNA; a step of producing a 4,200 cDNA chips containing 28 gene of beta-actin(Acc. No. DQ845171) and 18S(Acc. No. AF102857); and a step of mixing 254 gene having the significance in growth, meat quality and breeding character and 10 positive control genes to produce 264 cDNA chips.

    Abstract translation: 提供早期诊断与猪的生长,肉质和繁殖特性相关的基因的cDNA芯片,用于一次性分析猪的大量基因来源。 一种用于诊断与猪的生长,肉质和育种特性相关的基因的cDNA芯片的方法,包括:从猪的组织和血液制备组织样品的步骤; 从组织样品中分离RNA的步骤; 从RNA中克隆出4,172个EST的cDNA文库生成步骤; 生成含有β-肌动蛋白28基因(Acc。No.DD845171)和18S(Acc.No.AF102857)的4,200个cDNA芯片的步骤; 以及混合具有生长,肉质和育种特征的10种阳性对照基因的254基因的混合物的步骤,生成264个cDNA片段。

    유전자의 발현프로필을 이용한 돼지의 산자수 예측방법
    8.
    发明公开
    유전자의 발현프로필을 이용한 돼지의 산자수 예측방법 有权
    使用基因表达谱的SWINE FECUNDITY的预测方法

    公开(公告)号:KR1020160049177A

    公开(公告)日:2016-05-09

    申请号:KR1020140145448

    申请日:2014-10-24

    CPC classification number: C12Q1/6813 C12Q2600/124

    Abstract: 본발명은 DEG를활용한돼지의산자수예측방법에관한것이다. 본발명에서는산자수가우수한흑돼지품종을유전자원으로개량하기위해산자수가우수한모돈과산자수가열등한모돈의태반으로부터 RNA을분석하여 DEG(Differentially Expressed Gene)를얻고, 이들유전자와산자수와의연계성을정립하여산자수가우수한흑돼지계통조성을위한진단기술을제공한다.

    Abstract translation: 本发明涉及通过应用差异表达基因(DEG)来估计猪繁殖力的方法。 为了改善遗传资源,显示出优异繁殖力的黑猪品种,对来自较少繁殖母猪和高度繁殖母猪的胎盘的RNA进行分析,以获得DEG,并且 确定了基因和繁殖力,使得可以提供一种诊断技术,用于建立显示出优异繁殖力的黑猪谱系。

    조직별, 품종별, 성장단계별 유전자 발현양상 분석을 통하여 확보한 유전자를 이용하여 성장 또는 육질이 우수한 종돈을 조기에 선발하는 방법

    公开(公告)号:KR1020090065831A

    公开(公告)日:2009-06-23

    申请号:KR1020070133339

    申请日:2007-12-18

    CPC classification number: C12Q1/6827 C12Q1/6888 C12Q2600/124

    Abstract: A specific gene for diagnosing pig growth and the diagnosis of meat quality is provided to early select excellent pig species by obtaining the specific gene related to growth and meat quality. A method for obtaining a specific gene for diagnosing growth and meat quality of pig comprises: a step of building a cDNA library using muscle and fat tissue of Sus scrofa, designing and analyzing cDNA microarray; a step of collecting muscle and fat from Sus scrofa and Yorkshire and making tissue sample; a step of cloning a gene from the tissue sample; a step of analyzing full-sequence; a step of isolating total RNA; a step of synthesizing first strand cDNA using superscript II reverse transcriptase; and a step of performing statistical analysis.

    Abstract translation: 通过获得与生长和肉质相关的具体基因,提供了诊断猪生长和肉质诊断的具体基因,以早期选择优良的猪种。 用于获得用于诊断猪的生长和肉质的特定基因的方法包括:使用Sus scrofa的肌肉和脂肪组织构建cDNA文库的步骤,设计和分析cDNA微阵列; 从Sus scrofa和Yorkshire收集肌肉和脂肪并制作组织样本的步骤; 从组织样品中克隆基因的步骤; 分析全序列的步骤; 分离总RNA的步骤; 使用上标II逆转录酶合成第一链cDNA的步骤; 以及执行统计分析的步骤。

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