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公开(公告)号：KR1020130001479A
公开(公告)日：2013-01-04
申请号：KR1020110062259
申请日：2011-06-27
Applicant: 대한민국(관리부서:국립수산과학원) , 목포대학교산학협력단
Abstract: PURPOSE: A undaria peterseniana mass production method by inducing the regeneration and aging of undaria peterseniana free-living gametophytes is provided by securing the optimal condition for the regeneration and aging of the Undaria peterseniana free-living gametophytes. CONSTITUTION: Zoospores are released from an apothecium site of Undaria peterseniana. The released zoospores are cultured, and form gametophytes. Separate the gametophytes formed into female and male gametophytes. Culture the separated female and male gametophytes until the gametophytes reach the respective diameter of 3-10mm. Cut the cultured female and male gametophytes in the respective size of 50-400 micrometers. Culture the excised female and male gametophytes under the cell count of 3-25 cell/ind., the temperature condition of 5-20deg. C , the illumination of 5-40micromolm-2s-1, and the photoperiod of 10:14(Light:Dark)-14:10(L:D). Cross-fertilize the cultivated female and male gametophytes. Culture the gametophytes in a liquid culture medium of PESI(Provasoli`s enriched seawater media). Culture the female and male gametophytes until the gametophytes reach the diameter size of 5-6mm. Excise the female gametophytes in the size of 50-100 micrometers. Excise the male gametophytes in the size of 60-150 micrometers. Culture the female gametophytes under the cell count of 3-8 cell/ind., the temperature condition of 10-20 deg. C, the illumination of 15-30 micromolm-2s-1, and the photoperiod of 10:14(Light:Dark). Culture the male gametophytes under the cell count of 8-12 cell/ind., the temperature condition of 5-20 deg. C, the illumination of 15-25 micromolm-2s-1, and the photoperiod of 14:10(Light:Dark). [Reference numerals] (AA) Matured thallus of Undaria peterseniana; (BB) Rinsing the matured thallus, cut after washing, with sterilized seawater and ABM solution; (CC) Releasing zoospores at 10°C, 20umolm^-2s^-1, and 10:14h(L:D); (DD) 0.1ml zoospore solution; (EE) Separating the zoospores by dilution; (FF) Female gametophyte; (GG) Male gametophyte; (HH) Mass-producing clone gametophyte; (I1,I2) Cutting and regenerating every 20 days at 15°C, 20umolm^-2s^-1, and 10:14h(L:D); (JJ) Mixing the cut female and male gametophyte at the ratio of 1:1; (KK) Forming gamete for at least 15 days at 15°C, 20umolm^-2s^-1, and 14:10h(L:D); (LL) Forming young sporophyte for 40 days at 15°C, 60umolm^-2s^-1, and 14:10h(L:D)
Abstract translation: 目的：通过诱导无花果自由生存配子体的再生和衰老，通过确保无花果种子自由生存配子体的再生和衰老的最佳条件，提供了一种不育种的大量生产方法。 构成：动物孢子从黑腹果蝇的药膳部位释放出来。 释放的游动孢子被培养，形成配子体。 将形成的配子体分成雌雄配子体。 培养分离的雌雄配子，直到配子体达到3-10mm的相应直径。 切割培养的雌雄配子体大小为50-400微米。 培养切除的雌雄配子体细胞计数为3-25细胞/ ind，温度条件为5-20度。 C，5-40微摩尔-2s -1的照射，光照时间为10:14（Light：Dark）-14：10（L：D）。 对栽培的雌性和雄性配子进行交叉施肥。 在PESI（Provasoli富集海水培养基）的液体培养基中培养配子体。 培养雌雄配子，直到配子体直径达到5-6mm。 消除50-100微米尺寸的雌配子体。 消除大小为60-150微米的雄性配子体。 在3-8细胞/ ind的细胞计数下培养雌配子体，温度条件为10-20度。 C，照度为15-30微摩尔-2s -1，光周期为10:14（Light：Dark）。 培养雄性配子体细胞计数为8-12个细胞/ ind，温度条件为5-20度。 C，照射15-25微摩尔-2s -1，光周期为14:10（Light：Dark）。 （AA）成熟Un of属（Undaria peterseniana）; （BB）冲洗成熟的沙门氏菌，洗涤后切除，用灭菌海水和ABM溶液洗涤; （CC）在10℃，20umol ^ -2s ^ -1和10：14h（L：D）释放游动孢子; （DD）0.1ml游动液溶液; （EE）通过稀释分离游动孢子; （FF）雌配子体; （GG）雄配子体; （HH）大量生产克隆配子体; （I1，I2）在15℃，20umol ^ -2s ^ -1和10：14h（L：D）每20天切割和再生; （JJ）以1：1的比例混合切割的雌性和雄性配子体; （KK）在15℃，20umol ^ -2s ^ -1和14：10h（L：D）下成型配子至少15天; （LL）在15℃，60umol ^ -2s ^ -1和14：10h（L：D）形成年轻孢子体40天，
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公开(公告)号：KR1020130001398A
公开(公告)日：2013-01-04
申请号：KR1020110062110
申请日：2011-06-27
Applicant: 대한민국(관리부서:국립수산과학원) , 주식회사녹색식물연구소
CPC classification number: C12Q1/6827 , C12Q1/683 , C12Q2600/156
Abstract: PURPOSE: A method for determining Porphyra species using a CAPS(cleaved amplified polymorphic sequence) marker is provided to quickly and accurately distinguish 4 species including P. tenera, P. yezoensis, P. dentate, and P. seriata. CONSTITUTION: A primer set for distinguishing Porphyra species contains: one or more oligonucleotides selected from the group consisting of oligonucleotides containing 15 or more continuous nucleotides in sequence number 1; and one or more oligonucleotides selected from the group consisting of oligonucleotides containing 15 or more continuous nucleotides in sequence number 2. The primer set contains the ologinucleotides of sequence numbers 1 and 2. [Reference numerals] (AA) Cleaving restriction enzyme(HindIII); (BB) Analyzing diversity by electrophoresis-separation
Abstract translation: 目的：提供一种使用CAPS（切割扩增多态性序列）标记物确定紫菜属物种的方法，以快速，准确地区分四种，包括P. tenera，P. yezoensis，P. dentate和P. seriata。 构成：用于区分紫菜属物种的引物组包含：选自序列号1中含有15个或更多个连续核苷酸的寡核苷酸的一种或多种寡核苷酸; 和选自由序列号2中含有15个或更多个连续核苷酸的寡核苷酸组成的组中的一种或多种寡核苷酸。引物组含有序列号1和2的寡核苷酸。（符号）（AA）切割限制酶（HindIII）; （BB）通过电泳分离分析多样性
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公开(公告)号：KR101155994B1
公开(公告)日：2012-06-27
申请号：KR1020100070993
申请日：2010-07-22
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C07K14/405 , C12N15/11 , C12N15/63 , C12R1/89
Abstract: 본 발명은 김으로부터 유래된 것으로서, 세균류, 해조류 및 식물체에 고온내성을 부여하는 서열번호1의 단백질 및 이를 암호화하는 유전자 상기 유전자(서열번호2)를 포함하는 재조합벡터 및 상기 재조합벡터에 의한 형질전환체에 관한 것이다.
본 발명에 의하면, 온도가 올라가면 생육이 정지되어 생산량이 급감하는 경향이 있는 김에서 본 발명에 의한 유전자의 발현을 증대시킴으로써 고온내성을 부여하여 김의 생산성을 증대시킬 수 있다.
또한 바이오에너지 생산을 위한 다양한 해조류에 본 발명에 의한 유전자를 적용함으로써 해조류의 바이오메스의 생산을 증대시킬 수 있게 된다.-
公开(公告)号：KR1020120009130A
公开(公告)日：2012-02-01
申请号：KR1020100070993
申请日：2010-07-22
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C07K14/405 , C12N15/11 , C12N15/63 , C12R1/89
Abstract: PURPOSE: A laver-derived high temperature resistant protein in bacteria, algae, and plants and a gene encoding the protein are provided to enhance gene expression and laver production. CONSTITUTION: A protein which is isolated from laver and has high temperature resistance contains an amino acid sequence of sequence number 1. The protein is a 15kDa protein with 144 amino acids. A gene encoding the protein is a cDNA having a sequence of sequence number 2. A recombinant vector contains the gene, HTR2. A transformant is prepared by introducing the recombinant vector to the host such as bacteria, sea algae, or plants.
Abstract translation: 目的：提供细菌，藻类和植物中的紫菜衍生的高温抗性蛋白质和编码该蛋白质的基因，以增强基因表达和紫菜生产。 构成：从紫菜中分离并具有耐高温性的蛋白质含有序列号1的氨基酸序列。蛋白质是具有144个氨基酸的15kDa蛋白质。 编码蛋白质的基因是具有序列号2的序列的cDNA。重组载体含有基因HTR2。 通过将重组载体导入宿主例如细菌，海藻或植物来制备转化体。
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公开(公告)号：KR1020080064391A
公开(公告)日：2008-07-09
申请号：KR1020070001239
申请日：2007-01-05
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12N5/00
Abstract: A method for mass-producing gametophytes of Kjellmaniella crassifolia is provided to be usefully used for ecological restoring of the endangered Kjellmaniella crassifolia through mass-proliferation of the gametophytes and contribute to income increase of a fisherman by diversifying species of culture fishes. A method for mass-producing gametophytes of Kjellmaniella crassifolia comprises the steps of: (a) after cutting apothecia of Kjellmaniella crassifolia, removing impurities therefrom with sterile seawater; (b) inducing the apothecia to release zoospores; (c) germinating the released zoospores in a liquid culture medium or a solid culture medium to form gametophytes; (d) after aging the formed gametophytes in a solid medium, isolating them into female and male gametophytes; (e) homogenizing the isolated female and male gametophytes using a homogenizer and then cutting them to have a size of 10-50 micrometers; and (f) culturing the cut female gametophytes in a liquid medium at a temperature of 10-24 deg.C with the intensity of radiation of 15-70 muE/m^2xs^-1 and culturing the cut male gametophytes in a liquid medium at a temperature of 1-10 deg.C with the intensity of radiation of 40-80 muE/m^2xs^-1. A method for preserving the zoospores of the Kjellmaniella crassifolia comprises the steps of: (a) after cutting the apothecia of the Kjellmaniella crassifolia, removing impurities therefrom with sterile seawater; (b) inducing the apothecia to release the zoospores; and (c) preserving the released zoospores in a solid medium. Further, the liquid culture medium is Provasoli's enriched seawater media.
Abstract translation: 提供了一种大量生产拟南芥配子体的方法，有效地用于通过配子体的大规模扩散对濒危的ell for藜进行生态恢复，并通过文化鱼品种的多样化促进渔民的收入增长。 Kjellmaniella crassifolia的大规模生产配子体的方法包括以下步骤：（a）切割Kjellmaniella crassifolia的酱油后，用无菌海水除去杂质; （b）诱导酵母释放游动孢子; （c）在液体培养基或固体培养基中发芽释放的游动孢子以形成配子体; （d）在形成的配子体在固体培养基中老化后，将其分离成雌性和雄性配子体; （e）使用均化器均化分离的雌性和雄性配子体，然后将其切割成具有10-50微米的尺寸; 和（f）在10-24℃的温度下在液体培养基中培养切割的雌配子体，辐射强度为15-70μE/ m 2 2xs -1，并在液体培养基中培养切割的雄配子体 在1-10℃的温度下，辐射强度为40-80μE/ m ^ 2xs ^ -1。 一种保存Kjellmaniella crassifolia的游动孢子的方法，包括以下步骤：（a）在切割Kjellmaniella crassifolia的apothecia后，用无菌海水除去杂质; （b）诱导酵母释放动物园; 和（c）在固体培养基中保存释放的游动孢子。 此外，液体培养基是Provasoli富集的海水介质。
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Patent Agency Ranking
公开(公告)号：KR100764483B1
公开(公告)日：2007-10-09
申请号：KR1020060133355
申请日：2006-12-26
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A01K61/00
Abstract: A system for hatching fish eggs with a filtering type sterilizing device is provided to improve the hatching ratio, to solve the problems of the use of chemicals and to be used for indoor high-density production of fresh water fish. A system for hatching fish eggs with a filtering type sterilizing device includes a hatching tank, a fry tank, a water-collecting heating tank, a sterilizing/purifying unit and a moving unit. The fish eggs are hatched in the hatching tank. The hatched fries gather in the fry tank. The water discharged from the fry tank are collected and heated in the water-collecting heating tank. The sterilizing/purifying unit sterilizes and purifies the water that passed through the water-collecting heating tank. The moving unit is installed on a lower end of the system for hatching fish eggs. The hatching tank, the fry tank, and the water-collecting heating tank are made of transparent materials.
Abstract translation: 提供了一种用过滤式杀菌装置孵化鱼卵的系统，以提高孵化比例，解决化学品使用问题，并用于室内高密度生产淡水鱼。 用过滤式杀菌装置孵化鱼卵的系统包括：孵化罐，油箱，集水加热槽，消毒净化单元和移动单元。 鱼卵在孵化池中孵出。 孵出的薯条聚集在油炸罐中。 从油炸罐排出的水在集水加热槽中收集和加热。 消毒/净化单元对通过集水加热箱的水进行灭菌和净化。 移动单元安装在系统的下端，用于孵化鱼卵。 孵化罐，油炸罐和集水加热槽由透明材料制成。
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17.发明授权
公开(公告)号：KR101277246B1
公开(公告)日：2013-06-26
申请号：KR1020110120894
申请日：2011-11-18
Applicant: 대한민국(관리부서:국립수산과학원) , 목포대학교산학협력단
CPC classification number: Y02A40/88
Abstract: 본 발명은 넓미역(
Undariopsis
peterseniana ) 및 미역(
Undaria
pinnatifida )의 유리배우체를 이용한 이종교배 양식방법에 관한 것으로, 구체적으로 넓미역의 유리 배우체와 미역의 유리 배우체간의 이종교배를 위한 최적 조건을 확인하고 상기 조건을 이용하여 재배된 넓미역 및 미역 잡종이 넓미역 및 미역 순종과 비교하여 양식기간이 유의적으로 늘어나고, 생물량이 증가하며, 성장률이 빨라지므로, 전복산업을 포함한 다양한 바다양식을 위한 대형 갈조류 자원으로 유용하게 이용될 수 있다.-
公开(公告)号：KR1020130055241A
公开(公告)日：2013-05-28
申请号：KR1020110120894
申请日：2011-11-18
Applicant: 대한민국(관리부서:국립수산과학원) , 목포대학교산학협력단
Abstract: PURPOSE: A growing method of undariopsis peterseniana and a gametophyte is provided. CONSTITUTION: The sporophyte of undariopsis peterseniana and undaria pinnatifida releases zoospores. The released zoospores are grown to form a gametophyte. The gametophyte of undariopsis peterseniana and undaria pinnatifida is separated into female and male. Each of female and male gametophyte is grown until the diameter reaches 1-5mm. The grown gametophyte is homogenized and cut into 100-300μm. The cut female and male gametophyte are cultivated at a temperature of 5-20°C, a illumination of 5-40 M, and a photeperiod of 10:14(Light(L):Dark(D)) - 14:10(L:D). The grown undaria pinnatifida is cultivated with undariopsis petereniana. Each of male and female is grown until the diameter reaches 2-3mm.
Abstract translation: 目的：提供一种不断增长的黄芪和配子体的方法。 构成：未知的peterseniana和undaria pinnatifida的孢子体释放出游动孢子。 释放的游动孢子生长形成配子体。 未成熟的peterseniana和undaria pinnatifida的配子体分为女性和男性。 每个雌配子体和雄配子体生长直到直径达到1-5mm。 将成熟的配子体均质化并切成100-300μm。 切割的雌性和雄性配子体培养温度为5-20℃，照度为5-40M，光子浓度为10:14（Light（L）：Dark（D））-14:10（L ：D）。 种植的无性系具有不定形的petereniana。 每个雄性和雌性生长直到直径达到2-3mm。
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公开(公告)号：KR3003497970000S
公开(公告)日：2004-04-21
申请号：KR3020030016766
申请日：2003-06-16
Applicant: 부민종합건설 주식회사 , 대한민국(관리부서:국립수산과학원)
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公开(公告)号：KR200325792Y1
公开(公告)日：2003-09-06
申请号：KR2020030018892
申请日：2003-06-16
Applicant: 부민종합건설 주식회사 , 대한민국(관리부서:국립수산과학원)
IPC: A01K61/00
Abstract: 본 고안은 해중림을 조성하기 위한 인공어초에 관한 것으로, 상기 인공어초에 해조류의 서식면적을 넓히고 태양광선을 보다 잘 받을 수 있도록 일면을 경사지게 하고, 그 경사면 위에 다수의 돌출부를 형성하여 해조류의 포자가 쉽게 착생토록 하며, 또한 V형오목부를 형성하여 해조류의 가느다란 인공종묘사가 안전하게 착상시킴과 동시에 V형오목부의 개구부에 의해 적당한 해수의 유동을 제공하여 해조류의 생장에 유리한 환경을 제공하고, 측면과 배면에 직사각형의 오목부를 형성하여 패류의 은신처를 제공하며, 해중림(海中林)을 조성하여 수질정화 및 먹이사슬을 정상화시키고, 어·패류의 산란장 및 성육장 조성에 의한 해양생태계를 복원하여 황폐화된 연안어장을 회복하는데 그 효과가 있다.
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