公开(公告)号：KR100884086B1

公开(公告)日：2009-02-18

申请号：KR1020070040731

申请日：2007-04-26

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  임숙경 ,  박신영 ,  김재명 ,  김종만 ,  임춘태 ,  정석찬 ,  강승원 ,  삐아오찌앙슈 ,  쥬유린

IPC: A23K1/14 ,  A23K1/00

Abstract: 본 발명은 본제 1kg내에 황련추출물 500g(50 중량%); 연교추출물 100g(10 중량%); dextrose 300g(30 중량%); 감초추출물 100g(10 중량%를 포함하여 구성된 것을 특징으로 하는 황련 및 연교추출물을 이용한 사료첨가제 및 그 추출방법을 제공한다.
상기와 같은 본 발명은 연교 및 황련으로부터 얻어지는 추출물을 일정량을 사료에 첨가하여 가축들에게 급여하므로써, 가축의 일당증체량 및 사료효율 개선를 통한 성장촉진과 면역세포(림파구)의 증식을 통하여 면역증강 및 이물질이 가지는 병원균에 대한 항균특성을 이용하여 질병예방효과가 발현되도록 연교 및 황련을 첨가한 사료첨가제를 제공하므로 그에 따라 항생제가 들어있는 사료첨가제의 대용으로 활용되는 장점을 가지고 있다.
황련추출물, 연교추출물, 사료첨가제, 추출물

公开(公告)号：KR1020080114231A

公开(公告)日：2008-12-31

申请号：KR1020070063592

申请日：2007-06-27

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  김재명 ,  이희수 ,  장금찬 ,  박신영 ,  조윤상 ,  이명헌 ,  윤소미 ,  박중원 ,  지순옥 ,  정석찬

IPC: A61K36/758 ,  A61P37/02

CPC classification number: A61K36/758 ,  A23V2200/324 ,  A61K9/0019 ,  A61K9/14 ,  A61K2236/331 ,  A61K2236/39 ,  A61K2236/51 ,  Y10S514/885

Abstract: A method for manufacturing an immune enhancing composition using Zanthoxylli Fructus crust Fizeau polysaccharide extracts as an active ingredient is provided to have a superior immunostimulating activity, to enhance an immunogenicity of animal and to be used as an immunoadjuvant for animal, a vaccine throne and an adjuvant treating agent. A method for manufacturing an immune enhancing composition using Zanthoxylli Fructus crust Fizeau polysaccharide extracts as an active ingredient comprises steps of: obtaining crude extracts by heating Zanthoxylli Fructus crust; concentrating the extracts; and obtaining the polysaccharide fraction by filtering the condensed products.

Abstract translation: 本发明提供使用花椒壳多糖提取物作为活性成分的免疫增强组合物的制造方法，具有优异的免疫刺激活性，增强动物的免疫原性，并用作动物免疫佐剂，疫苗王位和佐剂 处理剂。 使用花椒壳多糖提取物作为活性成分制造免疫增强组合物的方法包括以下步骤：通过加热花椒油酯来获得粗提物; 浓缩提取物; 并通过过滤冷凝产物获得多糖级分。

公开(公告)号：KR1020090021610A

公开(公告)日：2009-03-04

申请号：KR1020070086250

申请日：2007-08-27

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  김재명 ,  이희수 ,  장금찬 ,  박신영 ,  조윤상 ,  이명헌 ,  윤소미 ,  박중원 ,  지순옥 ,  정석찬

IPC: A61K36/20 ,  A61P37/04

Abstract: Provided is an immunity improver using acer tegmentosum maximowocz extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunity improver using acer tegmentosum maximowocz extract comprises acer tegmentosum maximowocz extract as an active ingredient. A method for preparing the acer tegmentosum maximowocz extract includes a step of extracting the extract from the dried acer tegmentosum maximowocz at a temperature of 90~100°C by hot distilled-water.

Abstract translation: 本发明提供了一种免疫改良剂，其使用宏碁提取物，其改善对病原体的非特异性防御​​的功能，并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。 使用槭树提取物的抗微生物改良剂作为活性成分。 本发明提供了一种用于制备尖角果胶提取物的方法，其特征在于，在90〜100℃的温度下，通过热蒸馏水从干燥的青蒿中提取提取物。

公开(公告)号：KR1020090013599A

公开(公告)日：2009-02-05

申请号：KR1020070077854

申请日：2007-08-02

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  김재명 ,  이희수 ,  장금찬 ,  박신영 ,  조윤상 ,  이명헌 ,  윤소미 ,  박중원 ,  지순옥 ,  정석찬

IPC: A61K39/02 ,  A61P37/02 ,  A61K39/085

CPC classification number: A61K39/05 ,  C12R1/47 ,  Y10S435/887 ,  Y10S514/885

Abstract: An immunostimulant containing the lystic extract of Streptomyces albus is provided to reinforce the functions of various immune cells having the major role in the disease defense, thereby increasing the specific and nonspecific resistance to the disease infection of animals. The immunostimulant contains the lystic extract of Streptomyces albus which is prepared by cultivating Streptomyces albus in yeast malt extract agar broth at 26 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Streptomyces albus suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.

Abstract translation: 提供含有链霉菌（Streptomyces albus）的提取物的免疫刺激剂，以增强在疾病防御中具有主要作用的各种免疫细胞的功能，从而增加对动物疾病感染的特异性和非特异性抗性。 免疫刺激剂含有通过在26度的酵母麦芽提取物琼脂培养基中培养链霉菌（Streptomyces albus）而制备的链霉菌链霉菌的提取物。 培养72小时，将培养液以3000Xg离心30分钟并收集，用pH7.2的磷酸盐缓冲盐水（PBS）冲洗收集的沉淀物，并将漂洗的沉淀悬浮于PBS中，加入5％含有50mM Tris -HCl，0.15M NaCl和4％Triton x-114作为增溶剂加入到Streptomyces albus悬浮液中，在室温下搅拌混合物2-3天以溶解链霉菌，并将溶液的pH调节至6.5-7.2 ，并干燥恒温室中的溶液。

公开(公告)号：KR1020080093488A

公开(公告)日：2008-10-22

申请号：KR1020070037227

申请日：2007-04-17

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  이희수 ,  김재명 ,  임숙경 ,  박신영 ,  장금찬 ,  임춘태 ,  정석찬

IPC: A61K36/233 ,  A61P37/00

Abstract: An extract of Bupleuri Radix is provided to increase various activities of main immunocytes of an animal such as granulocyte and macrophage and induce strong defence capability on attach inoculation of pathogenic bacteria. A composition comprising the same extract is provided to be used as an animal medicine such as an animal immuno-enhancing agent and a vaccine adjuvant and a feed additive for treating and preventing animal diseases efficiently. A method for purifying an extract of Bupleuri Radix comprises the steps of: (a) heat-treating the Bupleuri Radix with hot water at a temperature of 80-120 deg.C for 2-3 hours to obtain a crude extract; (b) concentrating the crude extract; and (c) filtering the concentrate to obtain a polysaccharide fraction by filtering paper and microorganism filtering. An extract of Bupleuri Radix purified by the method is a Bupleuri Radix polysaccharide, of which sugar is a glycoprotein including a protein. A composition for increasing immunity comprises the extract of Bupleuri Radix as an active ingredient and a pharmaceutically acceptable carrier or an excipient. An animal immunity enhancing agent, a vaccine adjuvant, or a feed additive for an animal comprises the composition for increasing immunity as an active ingredient.

Abstract translation: 提供柴胡提取物，以增加动物如粒细胞和巨噬细胞的主要免疫细胞的各种活性，并在附着接种病原菌时诱导强大的防御能力。 提供包含相同提取物的组合物用作动物药物，例如动物免疫增强剂和疫苗佐剂和用于有效治疗和预防动物疾病的饲料添加剂。 纯化柴胡提取物的方法包括以下步骤：（a）在80-120℃的温度下用热水热处理柴胡，2-3小时，得到粗提物; （b）浓缩粗提物; 和（c）过滤浓缩物以通过滤纸和微生物过滤获得多糖级分。 通过该方法纯化的柴胡提取物是一种柴胡甘草多糖，其中糖是包含蛋白质的糖蛋白。 用于增加免疫力的组合物包含作为活性成分的柴胡提取物和药学上可接受的载体或赋形剂。 动物免疫增强剂，疫苗佐剂或用于动物的饲料添加剂包括用于增加免疫力作为活性成分的组合物。

公开(公告)号：KR1020080084384A

公开(公告)日：2008-09-19

申请号：KR1020070026062

申请日：2007-03-16

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  김종만 ,  임숙경 ,  김재명 ,  박신영 ,  임춘태 ,  장금찬 ,  정석찬

IPC: A61K36/238 ,  A61P37/02

Abstract: An extract of Saposhnikovia root is provided to improve immune response by increasing activities of neutrophil, macrophage and lymphocyte, production of cytokines and non-specific protection against pathogenic bacteria infection in animals, so that it is useful as immunomodulator and immunoadjuvant. An extract of Saposhnikovia root for improving immune response in animals is prepared by heating Saposhnikovia root in water, and administered into an animal, especially domestic animals including cow, pig and chicken in the amount of 10-20 mg/kg per day through oral administration, intravenous injection, intramuscular injection or subcutaneous injection.

Abstract translation: 提供Saposhnikovia根的提取物，通过增加嗜中性粒细胞，巨噬细胞和淋巴细胞的活性，产生细胞因子和非特异性保护免受动物中的致病细菌感染来改善免疫应答，从而可用作免疫调节剂和免疫佐剂。 通过在水中加热山茱萸碱来制备Saposhnikovia根的提取物，并通过口服给药至每天10-20mg / kg的动物，特别是包括牛，猪和鸡的家畜，其量为10-20mg / kg ，静脉注射，肌内注射或皮下注射。