개 파보바이러스 유전자형 감별진단용 PNA 마이크로어레이 칩 및 이를 이용한 감별진단법
    11.
    发明公开
    개 파보바이러스 유전자형 감별진단용 PNA 마이크로어레이 칩 및 이를 이용한 감별진단법 有权
    用于对犬类病毒基因组进行差异诊断的PNA微阵列和使用该方法进行诊断的方法

    公开(公告)号:KR1020110072039A

    公开(公告)日:2011-06-29

    申请号:KR1020090128806

    申请日:2009-12-22

    Abstract: PURPOSE: A PNA microarray chip for detecting canine parvovirus genotype is provided to enhance high sensitivity and to distinguish genotype. CONSTITUTION: A PNA microarray chip for determining and diagnosing canine parvovirus contains a peptide nucleic acid(PNA) of sequence numbers 1-6. A method for determining canine parvovirus genotype comprises: a step of isolating DNA from a sample; a step of amplifying a target DNA by PCR; a step of hybridizing the target DNA with PNA probe of sequence numbers 1-6; a step of scanning the resultant; and a step of determining genotype by reading scanning result.

    Abstract translation: 目的:提供用于检测犬细小病毒基因型的PNA微阵列芯片,以提高高灵敏度和区分基因型。 构成:用于确定和诊断犬细小病毒的PNA微阵列芯片含有序列号1-6的肽核酸(PNA)。 一种用于确定犬细小病毒基因型的方法包括:从样品中分离DNA的步骤; 通过PCR扩增靶DNA的步骤; 将靶DNA与序列号1-6的PNA探针杂交的步骤; 扫描结果的步骤; 以及通过读取扫描结果来确定基因型的步骤。

    소 타일레리아병에 대한 면역원성이 증진된 융합단백질, 및 그의 제조방법 및 용도
    12.
    发明公开
    소 타일레리아병에 대한 면역원성이 증진된 융합단백질, 및 그의 제조방법 및 용도 有权
    具有增强免疫原性的熔化蛋白,其制备方法及其用途的融合蛋白

    公开(公告)号:KR1020030053857A

    公开(公告)日:2003-07-02

    申请号:KR1020010083880

    申请日:2001-12-24

    Abstract: PURPOSE: A fusion protein having enhanced immunogenicity to bovine theileriosis, a preparation process thereof, and the use thereof are provided, thereby preventing the infection of bovine theileriosis. CONSTITUTION: A fusion protein having enhanced immunogenicity to bovine theileriosis is prepared by fusion of heat shock protein(HSP) with the amino-terminal of the surface antigen p33 of Theileria sergenti, wherein Theileria sergenti is Korean isolated Theileria sergenti, Sungwhan stock; the surface antigen p33 has the nucleotide sequence of SEQ ID NO: 1; and the heat shock protein(HSP) has the nucleotide sequence of SEQ ID NO: 2. A process for preparing the fusion protein having enhanced immunogenicity to bovine theileriosis comprises the steps of: constructing a recombinant vector containing the gene encoding the fusion protein of heat shock protein(HSP) and the surface antigen p33 of Theileria sergenti; preparing a transformed E. coli with the recombinant vector; and culturing the transformed E. coli.

    Abstract translation: 目的:提供具有增强的牛鳞状细胞病毒免疫原性的融合蛋白及其制备方法及其用途,从而防止牛皮肤病的感染。 构成:通过将热休克蛋白(HSP)与Theileria sergenti的表面抗原p33的氨基末端融合制备具有增强的牛鳞状细胞病毒免疫原性的融合蛋白,其中Theileria sergenti是韩国分离的Theileria sergenti,Sungwhan stock; 表面抗原p33具有SEQ ID NO:1的核苷酸序列; 热休克蛋白(HSP)具有SEQ ID NO:2的核苷酸序列。一种制备具有增强的牛鳞状细胞病免疫原性的融合蛋白的方法,包括以下步骤:构建含有编码热能融合蛋白的基因的重组载体 休克蛋白(HSP)和沙门氏菌的表面抗原p33; 用重组载体制备转化的大肠杆菌; 并培养转化的大肠杆菌。

    호기성 그람음성 식중독균 감별진단용 PNA 마이크로어레이 및 이를 이용하여 상기 균을 감별진단하는 방법
    15.
    发明公开
    호기성 그람음성 식중독균 감별진단용 PNA 마이크로어레이 및 이를 이용하여 상기 균을 감별진단하는 방법 有权
    用于微生物食物中毒性厌食菌的差异诊断的PNA微阵列及其使用差异诊断的方法

    公开(公告)号:KR1020120077360A

    公开(公告)日:2012-07-10

    申请号:KR1020100139293

    申请日:2010-12-30

    CPC classification number: C12Q1/689 C12Q1/6837 C12Q2525/107 C12R1/01

    Abstract: PURPOSE: A PNA microarray for identifying aerobic gram-negative food poisoning bacteria is provided to accurately and quickly identify and diagnose the bacteria from other bacteria. CONSTITUTION: A PNA microarray for identifying aerobic gram-negative bacteria contains a probe of sequence numbers 1-4 and a primer of sequence numbers 5-10. The aerobic gram-negative bacteria are identified using the PNA microarray. The bacteria include Salmonella spp., Vibrio parahaemolyticus, Escherichia coli O157:H7, or Yersinia enterocolitica.

    Abstract translation: 目的:提供用于识别有氧革兰氏阴性食物中毒细菌的PNA微阵列,以准确,快速地鉴定和诊断其他细菌的细菌。 构成:用于鉴定需氧革兰氏阴性细菌的PNA微阵列含有序列号1-4的探针和序列号5-10的引物。 使用PNA微阵列鉴定需氧革兰氏阴性细菌。 细菌包括沙门氏菌,副溶血弧菌,大肠杆菌O157:H7或小肠结肠炎耶尔森氏菌。

    호기성 그람양성 식중독균 감별진단용 PNA 마이크로어레이 및 이를 이용하여 상기 균을 감별진단하는 방법
    20.
    发明公开
    호기성 그람양성 식중독균 감별진단용 PNA 마이크로어레이 및 이를 이용하여 상기 균을 감별진단하는 방법 有权
    PNA微量元素用于差异性食物阳性食物毒素细菌的差异诊断及其使用差异诊断的方法

    公开(公告)号:KR1020120077359A

    公开(公告)日:2012-07-10

    申请号:KR1020100139292

    申请日:2010-12-30

    CPC classification number: C12Q1/689 C12Q1/6837 C12Q2525/107 C12R1/445

    Abstract: PURPOSE: A PNA microarray for identifying and diagnosing aerobic gram-positive food poisoning bacteria is provided to accurately and quickly diagnose Staphylococcus aureus and Listeria monocytogenes from other bacteria. CONSTITUTION: A PNA microarray for identifying and diagnosing aerobic gram-positive food poisoning bacteria contains a PNA(peptide nucleic acid) probe of sequence numbers 1 and 2 and a primer of sequence numbers 3-6. The aerobic gram-positive food poisoning bacteria include Staphylococcus aureus or Listeria monocytogenes.

    Abstract translation: 目的:提供用于识别和诊断好氧革兰氏阳性食物中毒细菌的PNA微阵列,以准确和快速地诊断来自其他细菌的金黄色葡萄球菌和单核细胞增多性李斯特菌。 构成:用于鉴定和诊断需氧革兰氏阳性食物中毒细菌的PNA微阵列含有序列号1和2的PNA(肽核酸)探针和序列号3-6的引物。 需氧革兰氏阳性食物中毒细菌包括金黄色葡萄球菌或单核细胞增多性李斯特氏菌。

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