Abstract:
PURPOSE: A PNA microarray chip for detecting canine parvovirus genotype is provided to enhance high sensitivity and to distinguish genotype. CONSTITUTION: A PNA microarray chip for determining and diagnosing canine parvovirus contains a peptide nucleic acid(PNA) of sequence numbers 1-6. A method for determining canine parvovirus genotype comprises: a step of isolating DNA from a sample; a step of amplifying a target DNA by PCR; a step of hybridizing the target DNA with PNA probe of sequence numbers 1-6; a step of scanning the resultant; and a step of determining genotype by reading scanning result.
Abstract:
PURPOSE: A fusion protein having enhanced immunogenicity to bovine theileriosis, a preparation process thereof, and the use thereof are provided, thereby preventing the infection of bovine theileriosis. CONSTITUTION: A fusion protein having enhanced immunogenicity to bovine theileriosis is prepared by fusion of heat shock protein(HSP) with the amino-terminal of the surface antigen p33 of Theileria sergenti, wherein Theileria sergenti is Korean isolated Theileria sergenti, Sungwhan stock; the surface antigen p33 has the nucleotide sequence of SEQ ID NO: 1; and the heat shock protein(HSP) has the nucleotide sequence of SEQ ID NO: 2. A process for preparing the fusion protein having enhanced immunogenicity to bovine theileriosis comprises the steps of: constructing a recombinant vector containing the gene encoding the fusion protein of heat shock protein(HSP) and the surface antigen p33 of Theileria sergenti; preparing a transformed E. coli with the recombinant vector; and culturing the transformed E. coli.
Abstract translation:目的:提供具有增强的牛鳞状细胞病毒免疫原性的融合蛋白及其制备方法及其用途,从而防止牛皮肤病的感染。 构成:通过将热休克蛋白(HSP)与Theileria sergenti的表面抗原p33的氨基末端融合制备具有增强的牛鳞状细胞病毒免疫原性的融合蛋白,其中Theileria sergenti是韩国分离的Theileria sergenti,Sungwhan stock; 表面抗原p33具有SEQ ID NO:1的核苷酸序列; 热休克蛋白(HSP)具有SEQ ID NO:2的核苷酸序列。一种制备具有增强的牛鳞状细胞病免疫原性的融合蛋白的方法,包括以下步骤:构建含有编码热能融合蛋白的基因的重组载体 休克蛋白(HSP)和沙门氏菌的表面抗原p33; 用重组载体制备转化的大肠杆菌; 并培养转化的大肠杆菌。
Abstract:
The present invention relates to a method for early diagnosis of necrotic enteritis by simply analyzing a level of toxin (Net B) generated from blood of chicken having necrotic enteritis by using Net B specific monoclonal antibodies and polyclonal antibodies and through ELISA.
Abstract:
본 발명은 호기성 그람양성 식중독균 감별진단용 PNA 마이크로어레이 및 이를 이용하여 상기 균을 감별진단하는 방법에 관한 것으로, 보다 상세하게는 서열번호 1, 2의 PNA(Peptide nucleic acid) 프로브 및 서열번호 3∼6의 프라이머를 포함함으로써 호기성 그람양성 식중독균인 황색포도상구균( Staphylococcus aureus ) 및 리스테리아 모노사이토제네스( Listeria monocytogenes )를 다른 세균들로부터 정확하고 신속하게 감별진단할 수 있는 PNA 마이크로어레이 및 이를 이용하여 상기 균을 감별진단하는 방법에 관한 것이다.
Abstract:
PURPOSE: A PNA microarray for identifying aerobic gram-negative food poisoning bacteria is provided to accurately and quickly identify and diagnose the bacteria from other bacteria. CONSTITUTION: A PNA microarray for identifying aerobic gram-negative bacteria contains a probe of sequence numbers 1-4 and a primer of sequence numbers 5-10. The aerobic gram-negative bacteria are identified using the PNA microarray. The bacteria include Salmonella spp., Vibrio parahaemolyticus, Escherichia coli O157:H7, or Yersinia enterocolitica.
Abstract:
The present invention relates to an application device installed in a portable terminal such as a smartphone to be independently driven and, more specifically, to a smartphone-based field epidemiological information analysis application device which can immediately provide epidemiological information required in the field, such as disease incidence calculation and sample size calculation.
Abstract:
본 발명은 닭의 괴사성 장염(necrotic enteritis)을 예방할 수 있는 백신 조성물에 관한 것으로, 보다 상세하게는 재조합 클로스트리디알 항원 단백질 Net-B와 PFO를 아쥬반트와 혼합하여 제조한 닭의 괴사성 장염 예방 백신 조성물에 관한 것이며, 본 발명의 백신은 닭의 괴사성 장염을 예방하는데 탁월한 효능이 있어 닭의 괴사성 장염의 발병을 감소시키며, 이를 통해 닭의 건강 증진뿐만 아니라 농가의 경제적 손실도 줄일 수 있다.
Abstract:
PURPOSE: A PNA microarray for identifying and diagnosing aerobic gram-positive food poisoning bacteria is provided to accurately and quickly diagnose Staphylococcus aureus and Listeria monocytogenes from other bacteria. CONSTITUTION: A PNA microarray for identifying and diagnosing aerobic gram-positive food poisoning bacteria contains a PNA(peptide nucleic acid) probe of sequence numbers 1 and 2 and a primer of sequence numbers 3-6. The aerobic gram-positive food poisoning bacteria include Staphylococcus aureus or Listeria monocytogenes.