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公开(公告)号：KR1020100087892A
公开(公告)日：2010-08-06
申请号：KR1020090006941
申请日：2009-01-29
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12N15/11 , C12Q1/6844 , C12Q2531/113
Abstract: PURPOSE: A method for detecting DNA polymorphism by real time PCR using SSR primer pair is provided to effectively detect DNA amplification polymorphism and to use in determining the species of Vigna angularis (Willd.) Ohwi and Ohashi. CONSTITUTION: A method for detecting DNA polymorphism comprises: a step of isolating genome DNA from Vigna angularis (Willd.) Ohwi and Ohashi; a step of performing real time PCR using SSR primer pair; and a step of separating PCR product. An SSR primer pair is a primer pair of sequence number 1 and 2, sequence numbers 3 and 4, or sequence numbers 5 and 6. The primer pair is derived from Vigna angularis (Willd.) Ohwi and Ohashi.
Abstract translation: 目的：提供使用SSR引物对通过实时PCR检测DNA多态性的方法，以有效检测DNA扩增多态性，并用于确定Vigna angularis（Willd。）Ohwi和Ohashi的种类。 构成：用于检测DNA多态性的方法包括：从Vigna angularis（Willd。）Ohwi和Ohashi分离基因组DNA的步骤; 使用SSR引物对进行实时PCR的步骤; 以及分离PCR产物的步骤。 SSR引物对是序列号1和序列号2，序列号3和4或序列号5和6的引物对。引物对来自Vigna angularis（Willd。）Ohwi和Ohashi。
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Patent Agency Ranking
公开(公告)号：KR1020100087891A
公开(公告)日：2010-08-06
申请号：KR1020090006938
申请日：2009-01-29
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12Q1/6844 , C12Q2600/156
Abstract: PURPOSE: An SSR primer isolated from Arachis hypogaea L. is provided to effectively detect the DNA polymorphism of peanuts and to be used for a DNA profile of Arachis hypogaea L. CONSTITUTION: An SSR primer pair isolated from Arachis hypogaea L. is a primer pair of sequence numbers 9 and 10, sequence numbers 11 and 12, sequence numbers 13 and 14, sequence numbers 15 and 16, sequence number 17 and 18, sequence numbers 19 and 20, sequence numbers 21 and 22, or sequence numbers 23 and 24. A method for detecting the DNA polymorphism of Arachis hypogaea L. comprises: a step of isolating a genome DNA from Arachis hypogaea L.; a step of performing PCR using an SSR primer pair; and a step of separating a PCR product.
Abstract translation: 目的：提供从花生花生中分离的SSR引物，有效检测花生的DNA多态性，并用于花生链球菌的DNA谱。构成：从花生链球菌分离的SSR引物对是引物对 的序列号9和10，序列号11和12，序列号13和14，序列号15和16，序列号17和18，序列号19和20，序列号21和22或序列号23和24。 用于检测花生梭菌DNA多态性的方法包括：分离花生梭菌L的基因组DNA的步骤。 使用SSR引物对进行PCR的步骤; 以及分离PCR产物的步骤。
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公开(公告)号：KR101166698B1
公开(公告)日：2012-07-27
申请号：KR1020080137194
申请日：2008-12-30
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 알파-글루코시데이즈 억제 활성을 갖는 팥 근연종 추출물에 관한 것으로서, 더욱 상세하게는 팥 근연종(
Vigna sp.) 추출물이 알파-글루코시데이즈의 강한 억제 효과를 나타냄으로 혈당 강하, 비만 억제 효과가 있고, 항산화 효과를 동시에 가지고 있어 당뇨병, 당뇨병성 질환 또는 비만 예방 및 치료 조성물로 의약품 및 건강기능성 식품 소재에 관한 것이다.
알파-글루코시데이즈, 팥 근연종(Vigna sp.) 추출물, 당뇨병, 당뇨병성 질환, 비만-
公开(公告)号：KR101161221B1
公开(公告)日：2012-07-20
申请号：KR1020090006938
申请日：2009-01-29
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 땅콩에서 분리한 SSR 프라이머 및 그의 용도에 관한 것으로, 더 상세하게는 서열번호 1 내지 서열번호 24로 이루어진 군에서 선택되는 2개의 염기서열을 갖는 SSR 프라이머쌍 및 이를 이용하여 땅콩의 DNA 다형성 검출 방법 및 땅콩 품종 동정방법에 관한 것이다.
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公开(公告)号：KR1020110009402A
公开(公告)日：2011-01-28
申请号：KR1020090066787
申请日：2009-07-22
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: An isoflavone content increasing method inside a bean seed is provided to use a lighting process to beans during the vegetative period of the beans. CONSTITUTION: An isoflavone content increasing method inside a bean seed includes a step of light-processing beans during the vegetative period of the beans. The intensity of the lighting process is 20~50lux. The lighting process is performed for 10~30days, 1~4hours after the sunrise and 1~3hours after the sunset. The beans are glycine max.
Abstract translation: 目的：在豆类种植期间提供异黄酮含量增加方法，以在豆类营养期内对豆类进行照明处理。 构成：豆类种子中异黄酮含量的增加方法包括在豆类营养期间轻度加工豆类的步骤。 照明过程的强度为20〜50lux。 照明过程进行10〜30天，日出后1〜4小时，日落后1〜3小时。 豆类最多为甘氨酸。
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公开(公告)号：KR1020100079527A
公开(公告)日：2010-07-08
申请号：KR1020080138049
申请日：2008-12-31
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6888 , C12Q2600/156
Abstract: PURPOSE: An SSR primer isolated from Vigna angularis (Willd.) Ohwi and Ohashi and a method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi using the same are provided to effectively detect DNA polymorphism and to determine the product species of Vigna angularis (Willd.) Ohwi and Ohashi. CONSTITUTION: An SSR(simple sequence repeat) primer pair comprises two base sequences selected from sequence numbers 1-12. The SSR primer pair is derived from Vigna angularis (Willd.) Ohwi and Ohashi. A method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi comprises: a step of extracting genome DNA from Vigna angularis (Willd.) Ohwi and Ohashi; a step of performing PCR using SSR primer; and a step of performing electrophoresis.
Abstract translation: 目的：从Vigna angularis（Willd。）Ohwi和Ohashi分离的SSR引物和用于检测Vigna angularis（Willd。）Ohwi和Ohashi的DNA多态性的方法，用于有效检测DNA多态性并确定产物种类 Vigna angularis（Willd。）Ohwi和Ohashi。 构成：SSR（简单序列重复）引物对包含从序列号1-12中选择的两个碱基序列。 SSR引物对衍生自Vigna angularis（Willd。）Ohwi和Ohashi。 用于检测Vigna angularis（Willd。）Ohwi和Ohashi的DNA多态性的方法包括：从Vigna angularis（Willd。）Ohwi和Ohashi提取基因组DNA的步骤; 使用SSR引物进行PCR的步骤; 和进行电泳的步骤。
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