Abstract:
PURPOSE: A method for enlarging the size of lily bulblet with low cost is provided, which has no effect on the growth of the plant and causes no environmental PROBLEM. CONSTITUTION: The method for enlarging the size of lily bulblet is characterized by the following steps of: i) growing ramentum and stem in medium; ii) cutting basal plate and multiplying the basal plate through subculture; iii) moving segments of multiplied basal plate to a bioreactor; and iv) cultivating the segments in liquid medium of the bioreactor. Wherein the liquid medium is MS (Murashige Skoog) medium containing 1g/L of activated carbon to absorb secretions of the plant and sucrose and is replaced every eight weeks.
Abstract:
PURPOSE: A method for inducing and proliferating somatic embryogenic callus is provided to develop a novel product of Rosa hybrida L. having effective characteristics. CONSTITUTION: A method for inducing somatic embryogenic callus from Rosa hybrida L. root comprises: a step of culturing Rosa hybrida L. in MS solid medium(pH 5.8), proliferating, and rooting; a step of culturing the fragment of the redifferentiated root in SH medium containing 5-8 mg/L of 2,4-dichlorophenoxyacetic acid for 2-8 weeks to induce callus; and a step of culturing the callus in SH medium to develop embryo for second callus induction.
Abstract translation:目的:提供诱导和增殖体细胞胚发生愈伤组织的方法,以开发具有有效特征的罗莎杂种L.的新产品。 构成:从罗莎杂交种根诱导体细胞胚发生愈伤组织的方法包括:在MS固体培养基(pH 5.8)中培养罗莎杂交L.增殖和生根的步骤; 在含有5-8mg / L 2,4-二氯苯氧基乙酸的SH培养基中培养再分化根的片段2-8周以诱导愈伤组织的步骤; 以及在SH培养基中培养愈伤组织以发展第二愈伤组织诱导的胚胎的步骤。