Abstract:
PURPOSE: A method for preparing extract derived from young barley leaf is provided to isolate novel ingredients and to be used as a functional material supply source. CONSTITUTION: A young barley leaf extract with an antioxidative activity is prepared using an organic solvent. The young barley leaf is collected in a length of 10-20 cm and is freeze-dried. The organic solvent is methanol or ethanol. The ethanol is 80-90 weight% ethanol solution. A composition for skin whitening or anti-aging contains the extract as an active ingredient. A health food for skin whitening or anti-aging contains the extract as an active ingredient. A solvent fraction of the extract contains one or more compounds selected from compound of chemical formulas 1-4.
Abstract:
PURPOSE: A primer set for determining white rice and colored rice is provided to determine homo/hetero type of characteristics and to enhance breeding efficiency. CONSTITUTION: A primer set for determining white rice and colored rice containing proanthocyanidin-based pigment comprises: an oligonucleotide primer set of sequence numbers 1 and 2; an oligonucleotide primer set of sequence numbers 3 and 4; an oligonucleotide primer set of sequence numbers 5 and 6; an oligonucleotide primer set of sequence numbers 7 and 4; and an oligonucleotide primer set of sequence numbers 8 and 6. A kit for determining the seed coat color of white rice and colored rice contains the primer set and reagent.
Abstract:
PURPOSE: A composition containing a DNA marker for selecting Oryza sativa with green rice leaf hopper-resistant gene Grh1 is provided to efficiently distinguish green rice leaf hopper resistant and sensitive individual. CONSTITUTION: A composition for selecting Oryza sativa species with green rice leaf hopper-resistance contains a primer for detecting the presence of an oligonucleotide or a fragment thereof. The primer is one or more primer sets selected from sequence numbers 1-14. The primer set is one or more primer sets selected from sequence numbers 3-14. A method for selecting the Oryza sativa species comprises: a step of isolating genome DNA from Oryza sativa samples; a step of amplifying a target sequence using the genome DNA as a template and the primer set; and a step of detecting the amplifies product. The sample is derived from roots, leaves, stems, flowers, or buds of Oryza sativa. A kit for selecting Oryza sativa species with green rice leaf hopper-resistance contains the primer set selected from sequence numbers 1-14, restriction enzyme, and reagent.