Abstract:
본 발명은 내부에 전극이 마련되어 있으며, 이 전극의 표면에는 PCR 프라이머 세트 중 제1 프라이머가 고정되는 증폭반응 챔버를 포함하며, 이 증폭반응 챔버 내에는 PCR 프라이머 세트 중 나노입자가 표지된 제2 프라이머가 첨가되는 마이크로 PCR 장치를 제공한다.
Abstract:
PURPOSE: A PCR primer set for detection of hepatitis B and a method for detecting hepatitis B using the same primer set are provided, thereby reducing the detection time to 30 minutes and improving the detection reproductivity by optimizing the PCR condition and using specific PCR primer set. CONSTITUTION: The PCR primer set for detection of hepatitis B is selected from the group consisting of: (a) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2; (b) a set of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:3; (c) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:5; (d) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:6; (e) a set of primers having the nucleotide sequences of SEQ ID NO:4 and SEQ ID NO:7; (f) a set of primers having the nucleotide sequences of SEQ ID NO:7 and SEQ ID NO:8; (g) a set of primers having the nucleotide sequences of SEQ ID NO:9 and SEQ ID NO:10; (h) a set of primers having the nucleotide sequences of SEQ ID NO:11 and SEQ ID NO:12; (i) a set of primers having the nucleotide sequences of SEQ ID NO:13 and SEQ ID NO:14; (j) a set of primers having the nucleotide sequences of SEQ ID NO:15 and SEQ ID NO:16; (k) a set of primers having the nucleotide sequences of SEQ ID NO:17 and SEQ ID NO:18; (l) a set of primers having the nucleotide sequences of SEQ ID NO:19 and SEQ ID NO:20; (m) a set of primers having the nucleotide sequences of SEQ ID NO:21 and SEQ ID NO:22; (n) a set of primers having the nucleotide sequences of SEQ ID NO:23 and SEQ ID NO:24; (o) a set of primers having the nucleotide sequences of SEQ ID NO:25 and SEQ ID NO:26; (p) a set of primers having the nucleotide sequences of SEQ ID NO:27 and SEQ ID NO:28; (q) a set of primers having the nucleotide sequences of SEQ ID NO:29 and SEQ ID NO:30; (r) a set of primers having the nucleotide sequences of SEQ ID NO:31 and SEQ ID NO:32; (s) a set of primers having the nucleotide sequences of SEQ ID NO:33 and SEQ ID NO:34; (t) a set of primers having the nucleotide sequences of SEQ ID NO:35 and SEQ ID NO:36; (u) a set of primers having the nucleotide sequences of SEQ ID NO:37 and SEQ ID NO:38; and (a) a set of primers having the nucleotide sequences of SEQ ID NO:39 and SEQ ID NO:40.
Abstract translation:目的:提供用于检测乙型肝炎的PCR引物组和使用相同引物组检测乙型肝炎的方法,从而将检测时间缩短到30分钟,并通过优化PCR条件并使用特异性PCR引物组来提高检测繁殖力 。 构成:用于检测乙型肝炎的PCR引物组选自:(a)一组具有SEQ ID NO:1和SEQ ID NO:2的核苷酸序列的引物; (b)具有SEQ ID NO:1和SEQ ID NO:3的核苷酸序列的一组引物; (c)具有SEQ ID NO:4和SEQ ID NO:5的核苷酸序列的一组引物; (d)具有SEQ ID NO:4和SEQ ID NO:6的核苷酸序列的一组引物; (e)具有SEQ ID NO:4和SEQ ID NO:7的核苷酸序列的一组引物; (f)具有SEQ ID NO:7和SEQ ID NO:8的核苷酸序列的一组引物; (g)具有SEQ ID NO:9和SEQ ID NO:10的核苷酸序列的一组引物; (h)具有SEQ ID NO:11和SEQ ID NO:12的核苷酸序列的一组引物; (i)具有SEQ ID NO:13和SEQ ID NO:14的核苷酸序列的一组引物; (j)具有SEQ ID NO:15和SEQ ID NO:16的核苷酸序列的一组引物; (k)具有SEQ ID NO:17和SEQ ID NO:18的核苷酸序列的一组引物; (1)具有SEQ ID NO:19和SEQ ID NO:20的核苷酸序列的一组引物; (m)具有SEQ ID NO:21和SEQ ID NO:22的核苷酸序列的一组引物; (n)具有SEQ ID NO:23和SEQ ID NO:24的核苷酸序列的一组引物; (o)具有SEQ ID NO:25和SEQ ID NO:26的核苷酸序列的一组引物; (p)一组具有SEQ ID NO:27和SEQ ID NO:28的核苷酸序列的引物; (q)具有SEQ ID NO:29和SEQ ID NO:30的核苷酸序列的一组引物; (r)具有SEQ ID NO:31和SEQ ID NO:32的核苷酸序列的一组引物; 一组具有SEQ ID NO:33和SEQ ID NO:34的核苷酸序列的引物; (t)具有SEQ ID NO:35和SEQ ID NO:36的核苷酸序列的一组引物; (u)具有SEQ ID NO:37和SEQ ID NO:38的核苷酸序列的一组引物; 和(a)具有SEQ ID NO:39和SEQ ID NO:40的核苷酸序列的一组引物。
Abstract:
The present invention relates to an organic/inorganic complex membrane wherein an organic macromolecular matrix having finger-like spiracles is dispersed with hydrophilic inorganic particles. The concentration of the inorganic particles inside the membrane is higher at a surface where the density of the membrane is higher than another surface where the density of the membrane is lower.
Abstract:
Provided are a separation membrane comprising a polymer including a structural unit represented by chemical formula 1; and a water treatment device using the separation membrane.
Abstract:
PURPOSE: A separation layer, a manufacturing method thereof, and a water treatment device using the same are provided to have an excellent durability with a high strength property, to minimize polarization of an inside concentration, to have an excellent salt removal rate, and to have a simple and economical manufacturing process. CONSTITUTION: A separation film comprises a supporting layer including a polymer with the structure unit represented by chemical formula 1, and a polymer matrix layer. In chemical formula 1, R1-R6 is independently hydrogen, or a substituted or non-substituted C1-C30 alkyl group, a substituted or non-substituted C3-C30 cycloalkyl group, a substituted or non-substituted C2-C30 heterocycloalkyl, a substituted or non-substituted C6-C30 aryl group, a substituted or non-substituted C2-C30 heteroaryl group, a substituted or non-substituted C7-C30 alkylaryl group, a substituted or non-substituted C7-C30 arylalkyl group, or -COR7. In chemical formula 1, R7 is a substituted or non-substituted C1-C30 alkyl group, a substituted or non-substituted C3-C30 cycloalkyl group, a substituted or non-substituted C2-C30 heterocycloalkyl, a substituted or non-substituted C6-C30 aryl group, a substituted or non-substituted C2-C30 heteroaryl group, a substituted or non-substituted C7-C30 alkylaryl group, or a substituted or non-substituted C7-C30 arylalkyl group. However, at least one among R1-R3 and at least one among R4-R6 are identical to or different from -COR7.
Abstract:
A method for indentifying a biomolecule is provided to indentify each DNA from one sample although various kinds of DNAs are PCR-amplified at the same time by indentifying A and B which are PCR products by utilizing transition temperature property. A biomolecule detecting apparatus having a field effect transistor(FET) is used in order to indentify a biomolecule. A method for indentifying a biomolecule comprises steps of: (a) heating a sample including the biomolecule into a biomolecule detecting apparatus in which the sample is injected; (b) measuring current flowing to a channel formed between a source region and a drain region of the field effect transistor; (c) calculating transition temperature which is temperature when current change amount is maximum from current data measured in the step (b); and (d) indentifying the biomolecule using the transition temperature.